Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes.

abstract：:RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular u...

journal_title：Nucleic acids research

authors： Hermann T,Schmid B,Heumann H,Göringer HU

更新日期：1997-06-15 00:00:00

abstract：:To organize data resulting from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database (http://urgi.versailles.inra.fr/OryzaTagLine/). OTL structure facilitates forward genetic search for sp...

journal_title：Nucleic acids research

authors： Larmande P,Gay C,Lorieux M,Périn C,Bouniol M,Droc G,Sallaud C,Perez P,Barnola I,Biderre-Petit C,Martin J,Morel JB,Johnson AA,Bourgis F,Ghesquière A,Ruiz M,Courtois B,Guiderdoni E

更新日期：2008-01-01 00:00:00

abstract：:Thymine DNA glycosylase (TDG) is a DNA repair enzyme that excises a variety of mismatched or damaged nucleotides (nts), e.g. dU, dT, 5fC and 5caC. TDG is shown to play essential roles in maintaining genome integrity and correctly programming epigenetic modifications through DNA demethylation. After locating the lesion...

journal_title：Nucleic acids research

authors： Da LT,Yu J

更新日期：2018-06-20 00:00:00

abstract：:The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA ...

journal_title：Nucleic acids research

authors： Rafalski A,Kohli J,Agris P,Söll D

更新日期：1979-06-25 00:00:00

abstract：:The primary function of microRNAs (miRNAs) is to maintain cell homeostasis. In cancerous tissues miRNAs' expression undergo drastic alterations. In this study, we use miRNA expression profiles from The Cancer Genome Atlas of 24 cancer types and 3 healthy tissues, collected from >8500 samples. We seek to classify the c...

journal_title：Nucleic acids research

authors： Rasnic R,Linial N,Linial M

更新日期：2017-05-19 00:00:00

abstract：:Real-time PCR is being used increasingly as the method of choice for mRNA quantification, allowing rapid analysis of gene expression from low quantities of starting template. Despite a wide range of approaches, the same principles underlie all data analysis, with standard approaches broadly classified as either absolu...

journal_title：Nucleic acids research

authors： Peirson SN,Butler JN,Foster RG

更新日期：2003-07-15 00:00:00

abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...

journal_title：Nucleic acids research

authors： Wang G,Xu X,Pace B,Dean DA,Glazer PM,Chan P,Goodman SR,Shokolenko I

更新日期：1999-07-01 00:00:00

abstract：:Escherichia coli DNA polymerase III holoenzyme is composed of 10 different subunits linked by noncovalent interactions. The polymerase activity resides in the alpha-subunit. The epsilon-subunit, which contains the proofreading exonuclease site within its N-terminal 185 residues, binds to alpha via a segment of 57 addi...

journal_title：Nucleic acids research

authors： Ozawa K,Jergic S,Park AY,Dixon NE,Otting G

更新日期：2008-09-01 00:00:00

abstract：:The availability of the complete sequence of the Saccharomyces cerevisiae genome has allowed a comprehensive analysis of the genes encoding cytoplasmic ribosomal proteins in this organism. On the basis of this complete inventory a new nomenclature for the yeast ribosomal proteins is presented. ...

journal_title：Nucleic acids research

authors： Mager WH,Planta RJ,Ballesta JG,Lee JC,Mizuta K,Suzuki K,Warner JR,Woolford J

更新日期：1997-12-15 00:00:00

abstract：:Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That ...

journal_title：Nucleic acids research

authors： Kim H,Kang N,Chon KW,Kim S,Lee N,Koo J,Kim MS

更新日期：2015-11-16 00:00:00

abstract：:The apolipoprotein AI (apoAI) promoter/enhancer contains multiple cis -acting elements on which a variety of hepatocyte-enriched and ubiquitous transcription factors function synergistically to regulate liver-specific transcription. Adenovirus E1A proteins repress tissue-specific gene expression and disrupt the differ...

journal_title：Nucleic acids research

authors： Kilbourne EJ,Evans MJ,Karathanasis SK

更新日期：1998-04-01 00:00:00

abstract：:The wobble uridine of certain bacterial and mitochondrial tRNAs is modified, at position 5, through an unknown reaction pathway that utilizes the evolutionarily conserved MnmE and GidA proteins. The resulting modification (a methyluridine derivative) plays a critical role in decoding NNG/A codons and reading frame mai...

journal_title：Nucleic acids research

authors： Moukadiri I,Prado S,Piera J,Velázquez-Campoy A,Björk GR,Armengod ME

更新日期：2009-11-01 00:00:00

abstract：:We identify a new subgroup of Type I Restriction-Modification enzymes that modify cytosine in one DNA strand and adenine in the opposite strand for host protection. Recognition specificity has been determined for ten systems using SMRT sequencing and each recognizes a novel DNA sequence motif. Previously characterized...

journal_title：Nucleic acids research

authors： Morgan RD,Luyten YA,Johnson SA,Clough EM,Clark TA,Roberts RJ

更新日期：2016-11-02 00:00:00

abstract：:The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...

journal_title：Nucleic acids research

authors： Jordan BR,Latil-Damotte M,Jourdan R

更新日期：1980-08-25 00:00:00

abstract：:Invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. However, recent data suggest a relax control of replication timing. To evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regula...

journal_title：Nucleic acids research

authors： Bénard M,Maric C,Pierron G

更新日期：2007-01-01 00:00:00

abstract：:The Xist lncRNA requires Repeat A, a conserved RNA element located in its 5' end, to induce gene silencing during X-chromosome inactivation. Intriguingly, Repeat A is also required for production of Xist. While silencing by Repeat A requires the protein SPEN, how Repeat A promotes Xist production remains unclear. We r...

journal_title：Nucleic acids research

authors： Trotman JB,Lee DM,Cherney RE,Kim SO,Inoue K,Schertzer MD,Bischoff SR,Cowley DO,Calabrese JM

更新日期：2020-10-09 00:00:00

abstract：:MolProbity is a general-purpose web server offering quality validation for 3D structures of proteins, nucleic acids and complexes. It provides detailed all-atom contact analysis of any steric problems within the molecules as well as updated dihedral-angle diagnostics, and it can calculate and display the H-bond and va...

journal_title：Nucleic acids research

authors： Davis IW,Leaver-Fay A,Chen VB,Block JN,Kapral GJ,Wang X,Murray LW,Arendall WB 3rd,Snoeyink J,Richardson JS,Richardson DC

更新日期：2007-07-01 00:00:00

abstract：:The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue- specific genes. In this study the regulation of Hex promoter activity was investigated. Using co- transfection experiments, we demonstrated that the transcriptiona...

journal_title：Nucleic acids research

authors： Puppin C,D'Elia AV,Pellizzari L,Russo D,Arturi F,Presta I,Filetti S,Bogue CW,Denson LA,Damante G

更新日期：2003-04-01 00:00:00

abstract：:2'-O-(2-methoxyethyl) (2'-MOE) RNA possesses favorable pharmocokinetic properties that make it a promising option for the design of oligonucleotide drugs. Telomerase is a ribonucleoprotein that is up-regulated in many types of cancer, but its potential as a target for chemotherapy awaits the development of potent and ...

journal_title：Nucleic acids research

authors： Elayadi AN,Demieville A,Wancewicz EV,Monia BP,Corey DR

更新日期：2001-04-15 00:00:00

abstract：:Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant ba...

journal_title：Nucleic acids research

authors： Li N,Chen Y,Guo Q,Zhang Y,Yuan Y,Ma C,Deng H,Lei J,Gao N

更新日期：2013-08-01 00:00:00

abstract：:We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...

journal_title：Nucleic acids research

authors： Potrykus K,Murphy H,Chen X,Epstein JA,Cashel M

更新日期：2010-03-01 00:00:00

abstract：:In the last decade polycistronic vectors have become essential tools for both basic science and gene therapy applications. In order to co-express heterologous polypeptides, different systems have been developed from Internal Ribosome Entry Site (IRES) based vectors to the use of the 2A peptide. Unfortunately, these me...

journal_title：Nucleic acids research

authors： Fallot S,Ben Naya R,Hieblot C,Mondon P,Lacazette E,Bouayadi K,Kharrat A,Touriol C,Prats H

更新日期：2009-11-01 00:00:00

abstract：:Both Streptomyces lividans and Streptomyces avermitilis encode similar systems of post-replicative DNA modification which act site-specifically on closely opposed guanines on either strand. The modifications can be detected since they react in vitro with an oxidative derivative of Tris, resulting in strand cleavage. P...

journal_title：Nucleic acids research

authors： Boybek A,Ray TD,Evans MC,Dyson PJ

更新日期：1998-07-15 00:00:00

abstract：:Recent research hints at an underappreciated complexity in pre-miRNA processing and regulation. Global profiling of pre-miRNA and its potential to increase understanding of the pre-miRNA landscape is impeded by overlap with highly expressed classes of other non coding (nc) RNA. Here, we present a data set excluding th...

journal_title：Nucleic acids research

authors： Burroughs AM,Kawano M,Ando Y,Daub CO,Hayashizaki Y

更新日期：2012-02-01 00:00:00

abstract：:Rotaviruses are a major cause of acute, often fatal, gastroenteritis in infants and young children world-wide. Virions contain an 11 segment double-stranded RNA genome. Little is known about the cis-acting sequences and structural elements of the viral RNAs. Using a database of 1621 full-length sequences of mammalian ...

journal_title：Nucleic acids research

authors： Li W,Manktelow E,von Kirchbach JC,Gog JR,Desselberger U,Lever AM

更新日期：2010-11-01 00:00:00

abstract：:We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association w...

journal_title：Nucleic acids research

authors： Belikov SV,Belgovsky AI,Preobrazhenskaya OV,Karpov VL,Mirzabekov AD

更新日期：1993-02-25 00:00:00

abstract：:The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to syn...

journal_title：Nucleic acids research

authors： Buvoli M,Cobianchi F,Biamonti G,Riva S

更新日期：1990-11-25 00:00:00

abstract：:The third intron from Physarum polycephalum (Pp LSU 3) is one of the closest known relatives to the well-studied Tetrahymena group I intron. Both introns are located at the same position in the 26S rRNA gene, and with the exception of an open reading frame in Pp LSU 3, are highly homologous. While Pp LSU 3 has been sh...

journal_title：Nucleic acids research

authors： Rocheleau GA,Woodson SA

更新日期：1994-10-11 00:00:00

abstract：:Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with ...

journal_title：Nucleic acids research

authors： Gubbins EJ,Maurer RA,Hartley JL,Donelson JE

更新日期：1979-03-01 00:00:00

abstract：:The Metabolic Pathway Collection from EMP is an extraction of data from the larger Enzymes and Metabolic Pathways database (EMP). This extraction has been made publicly available in the hope that others will find it useful for a variety of purposes. The original release in October 1995 contained 1814 distinct pathways...

journal_title：Nucleic acids research

authors： Selkov E,Galimova M,Goryanin I,Gretchkin Y,Ivanova N,Komarov Y,Maltsev N,Mikhailova N,Nenashev V,Overbeek R,Panyushkina E,Pronevitch L,Selkov E Jr

更新日期：1997-01-01 00:00:00