Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.

abstract：:Two new crescent-shaped unsymmetrical cyanine dyes have been synthesised and their interactions with DNA have been investigated by different spectroscopic methods. These dyes are analogues to a minor groove binding unsymmetrical cyanine dye, BEBO, recently reported by us. In this dye, the structure of the known interc...

journal_title：Nucleic acids research

authors： Karlsson HJ,Eriksson M,Perzon E,Akerman B,Lincoln P,Westman G

更新日期：2003-11-01 00:00:00

abstract：:Negative superhelical strain induces the poly(dG)-poly(dC) sequence to adopt two totally different types of triple-helices, either a dG.dG.dC triplex in the presence of Mg(+)+ at both neutral and acidic pHs or a protonated dC+.dG.dC triplex in the absence of Mg(+)+ ions at acidic pH (1). To examine whether there are s...

journal_title：Nucleic acids research

authors： Kohwi-Shigematsu T,Kohwi Y

更新日期：1991-08-11 00:00:00

abstract：:The alpha/beta-hydrolase fold is characterized by a beta-sheet core of five to eight strands connected by alpha-helices to form a alpha/beta/alpha sandwich. In most of the family members the beta-strands are parallels, but some show an inversion in the order of the first strands, resulting in antiparallel orientation....

journal_title：Nucleic acids research

authors： Hotelier T,Renault L,Cousin X,Negre V,Marchot P,Chatonnet A

更新日期：2004-01-01 00:00:00

abstract：:The potency of the immune response has still to be harnessed effectively to combat human cancers. However, the discovery of T-cell targets in melanomas and other tumors has raised the possibility that cancer vaccines can be used to induce a therapeutically effective immune response against cancer. The targets, cancer-...

journal_title：Nucleic acids research

authors： Almeida LG,Sakabe NJ,deOliveira AR,Silva MC,Mundstein AS,Cohen T,Chen YT,Chua R,Gurung S,Gnjatic S,Jungbluth AA,Caballero OL,Bairoch A,Kiesler E,White SL,Simpson AJ,Old LJ,Camargo AA,Vasconcelos AT

更新日期：2009-01-01 00:00:00

abstract：:A novel expression system was established that allows expression and propagation of the human hepatitis B virus (HBV) genome in cultured animal cells. An overlength HBV genome encoding the viral pregenomic RNA was put under transcriptional control of the human metallothionein IIA promoter thereby replacing the endogen...

journal_title：Nucleic acids research

authors： Junker M,Galle P,Schaller H

更新日期：1987-12-23 00:00:00

abstract：:The enterovirus 71 (EV71) 3Dpol is an RNA-dependent RNA polymerase (RdRP) that plays the central role in the viral genome replication, and is an important target in antiviral studies. Here, we report a crystal structure of EV71 3Dpol elongation complex (EC) at 1.8 Å resolution. The structure reveals that the 5'-end gu...

journal_title：Nucleic acids research

authors： Shi W,Ye HQ,Deng CL,Li R,Zhang B,Gong P

更新日期：2020-02-20 00:00:00

abstract：:VIDA is a new virus database that organizes open reading frames (ORFs) from partial and complete genomic sequences from animal viruses. Currently VIDA includes all sequences from GenBank for Herpesviridae, Coronaviridae and Arteriviridae. The ORFs are organized into homologous protein families, which are identified on...

journal_title：Nucleic acids research

authors： Albà MM,Lee D,Pearl FM,Shepherd AJ,Martin N,Orengo CA,Kellam P

更新日期：2001-01-01 00:00:00

abstract：:The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should ...

journal_title：Nucleic acids research

authors： Leneuve P,Colnot S,Hamard G,Francis F,Niwa-Kawakita M,Giovannini M,Holzenberger M

更新日期：2003-03-01 00:00:00

abstract：:Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of ...

journal_title：Nucleic acids research

authors： Dufour D,de la Peña M,Gago S,Flores R,Gallego J

更新日期：2009-02-01 00:00:00

abstract：:The continuing threat of infectious disease and future pandemics, coupled to the continuous increase of drug-resistant pathogens, makes the discovery of new and better vaccines imperative. For effective vaccine development, antigen discovery and validation is a prerequisite. The compilation of information concerning p...

journal_title：Nucleic acids research

authors： Ansari HR,Flower DR,Raghava GP

更新日期：2010-01-01 00:00:00

abstract：:The Structural Classification of Proteins (SCOP) database is a comprehensive ordering of all proteins of known structure, according to their evolutionary and structural relationships. Protein domains in SCOP are hierarchically classified into families, superfamilies, folds and classes. The continual accumulation of se...

journal_title：Nucleic acids research

authors： Andreeva A,Howorth D,Brenner SE,Hubbard TJ,Chothia C,Murzin AG

更新日期：2004-01-01 00:00:00

abstract：:The vertebrate genome is a mosaic of regions differing dramatically in their G + C content. Those regions with a high G + C content contain the expected number of CpG dinucleotides and we propose that following methylation these have been protected from deamination by the increased stability of the surrounding DNA dup...

journal_title：Nucleic acids research

authors： Adams RL,Eason R

更新日期：1984-07-25 00:00:00

abstract：:The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which...

journal_title：Nucleic acids research

authors： Fleck O,Michael H,Heim L

更新日期：1992-05-11 00:00:00

abstract：:Protein-based methods of siRNA delivery are capable of uniquely specific targeting, but are limited by technical challenges such as low potency or poor biophysical properties. Here, we engineered a series of ultra-high affinity siRNA binders based on the viral protein p19 and developed them into siRNA carriers targete...

journal_title：Nucleic acids research

authors： Yang NJ,Kauke MJ,Sun F,Yang LF,Maass KF,Traxlmayr MW,Yu Y,Xu Y,Langer RS,Anderson DG,Wittrup KD

更新日期：2017-07-27 00:00:00

abstract：:Polyketide synthases (PKSs) catalyze biosynthesis of a diverse family of pharmaceutically important secondary metabolites. Bioinformatics analysis of sequence and structural features of PKS proteins plays a crucial role in discovery of new natural products by genome mining, as well as in design of novel secondary meta...

journal_title：Nucleic acids research

authors： Anand S,Prasad MV,Yadav G,Kumar N,Shehara J,Ansari MZ,Mohanty D

更新日期：2010-07-01 00:00:00

abstract：:The sequence of a DNA fragment about 1 Kbp long located at the 3' boundary of the chicken alpha globin gene domain, including the 3'-side matrix attachment point and the site of transcription termination, was determined. It contains a repetitive DNA element and the AT-rich (easily denaturable) DNA segment conserved at...

journal_title：Nucleic acids research

authors： Farache G,Razin SV,Targa FR,Scherrer K

更新日期：1990-02-11 00:00:00

abstract：:DNA restriction fragments located 5' to the human c-myc gene display anomalous electrophoretic mobility on polyacrylamide gels. Computer modeling of the c-myc flanking DNA suggests that the slow-moving DNA fragments spanning nucleotides -1690 to -1054 (relative to c-myc promoter P1) and -718 to -452 form large left ha...

journal_title：Nucleic acids research

authors： Kumar S,Leffak M

更新日期：1989-04-11 00:00:00

abstract：:Transcriptional regulation of the delta-aminolevulinic acid synthetase gene of Rhizobium meliloti was investigated under conditions of normal vegetative growth and during symbiosis with the legume host alfalfa. S1 nuclease mapping and DNA sequence analysis indicated that transcription originates from two sites separat...

journal_title：Nucleic acids research

authors： Leong SA,Williams PH,Ditta GS

更新日期：1985-08-26 00:00:00

abstract：:GenBank(®) (http://www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for over 300 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing proj...

journal_title：Nucleic acids research

authors： Benson DA,Clark K,Karsch-Mizrachi I,Lipman DJ,Ostell J,Sayers EW

更新日期：2015-01-01 00:00:00

abstract：:Full-length cDNA of the 6.3 kb turnip yellow mosaic virus (TYMV) genome was placed between a T7 promoter and a unique Hind III site. In vitro transcription of Hind III-linearized DNA of clone pTYMC yielded full-length RNA transcripts. In inoculations of Chinese cabbage protoplasts and plants, capped transcripts and vi...

journal_title：Nucleic acids research

authors： Weiland JJ,Dreher TW

更新日期：1989-06-26 00:00:00

abstract：:The plant gene enod40 is highly conserved among legumes and also present in various non-legume species. It is presumed to play a central regulatory role in the Rhizobium-legume interaction, being expressed well before the initiation of cortical cell divisions resulting in nodule formation. Two small peptides encoded b...

journal_title：Nucleic acids research

authors： Girard G,Roussis A,Gultyaev AP,Pleij CW,Spaink HP

更新日期：2003-09-01 00:00:00

abstract：:The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD act...

journal_title：Nucleic acids research

authors： Smith CA,Shingler V,Thomas CM

更新日期：1984-04-25 00:00:00

abstract：:U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript. The U6 gene of the fission yeast Schizosaccharomyces pombe is unusual in that it is interrupted by an intron whose structure is similar to ...

journal_title：Nucleic acids research

authors： Potashkin J,Frendewey D

更新日期：1989-10-11 00:00:00

abstract：:To gain insight into the mechanistic link between translation termination and nonsense-mediated mRNA decay (NMD), we depleted the ribosome recycling factor ABCE1 in human cells, resulting in an upregulation of NMD-sensitive mRNAs. Suppression of NMD on these mRNAs occurs prior to their SMG6-mediated endonucleolytic cl...

journal_title：Nucleic acids research

authors： Annibaldis G,Domanski M,Dreos R,Contu L,Carl S,Kläy N,Mühlemann O

更新日期：2020-10-09 00:00:00

abstract：:In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase ...

journal_title：Nucleic acids research

authors： Fodor I,Matvienko NI,Zlotnikov KM,Tanyashin VI,Solonin AS,Bayev AA

更新日期：1975-05-01 00:00:00

abstract：:Expansions of (GAA)n repeats within the first intron of the frataxin gene reduce its expression, resulting in a hereditary neurodegenerative disorder, Friedreich's ataxia. While it is generally believed that expanded (GAA)n repeats block transcription elongation, fine mechanisms responsible for gene repression are not...

journal_title：Nucleic acids research

authors： Krasilnikova MM,Kireeva ML,Petrovic V,Knijnikova N,Kashlev M,Mirkin SM

更新日期：2007-01-01 00:00:00

abstract：:Mitochondrial proteins remain the subject of intense research interest due to their implication in an increasing number of different conditions including mitochondrial and metabolic disease, cancer, and neuromuscular degenerative and age-related disorders. However, the mitochondrial proteome has yet to be accurately a...

journal_title：Nucleic acids research

authors： Smith AC,Robinson AJ

更新日期：2016-01-04 00:00:00

abstract：:The Saccharomyces cerevisiae viruses are non-infectious double-stranded (ds) RNA viruses present in most laboratory strains of yeast. Their genome consists of one or more dsRNAs separately encapsidated in particles composed mainly of one polypeptide, which has a Mr of 88 kdaltons in the best-studied viral subtype. A l...

journal_title：Nucleic acids research

authors： Bruenn J,Madura K,Siegel A,Miner Z,Lee M

更新日期：1985-03-11 00:00:00

abstract：:Fluorescence anisotropy and fluorescence correlation spectroscopy measurements of rhodamine-labeled DNA oligonucleotide duplexes have been used to determine equilibrium binding constants for DNA binding of the prokaryotic transcription activator protein NtrC. Measurements were made with wild-type NtrC from Escherichia...

journal_title：Nucleic acids research

authors： Sevenich FW,Langowski J,Weiss V,Rippe K

更新日期：1998-03-15 00:00:00

abstract：:Transcription and translation are coupled in bacteria, meaning that translation takes place co-transcriptionally. During transcription-translation, both machineries mutually affect each others' functions, which is important for regulation of gene expression. Analysis of interactions between RNA polymerase (RNAP) and t...

journal_title：Nucleic acids research

authors： Castro-Roa D,Zenkin N

更新日期：2012-03-01 00:00:00