Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins.

abstract：:Ribonuclease MRP is an endonuclease, related to RNase P, which functions in eukaryotic pre-rRNA processing. In Saccharomyces cerevisiae, RNase MRP comprises an RNA subunit and ten proteins. To improve our understanding of subunit roles and enzyme architecture, we have examined protein-protein and protein-RNA interacti...

journal_title：Nucleic acids research

authors： Aspinall TV,Gordon JM,Bennett HJ,Karahalios P,Bukowski JP,Walker SC,Engelke DR,Avis JM

更新日期：2007-01-01 00:00:00

abstract：:Pre-mRNA splicing is catalyzed by the spliceosome, a multi-megadalton ribonucleoprotein machine. Previous work from our laboratory revealed the splicing factor SRSF1 as a regulator of the SUMO pathway, leading us to explore a connection between this pathway and the splicing machinery. We show here that addition of a r...

journal_title：Nucleic acids research

authors： Pozzi B,Bragado L,Will CL,Mammi P,Risso G,Urlaub H,Lührmann R,Srebrow A

更新日期：2017-06-20 00:00:00

abstract：:The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...

journal_title：Nucleic acids research

authors： Swaminathan S,Malhotra P,Manohar CF,Dhar R,Thimmapaya B

更新日期：1993-06-11 00:00:00

abstract：:The stable intermediates formed in the reaction of cytosine, cytidine and adenosine with chloracetaldehyde were isolated. The -CH2CH/OH/- bridge between the exo and endo nitrogen atoms of the parent base was found in these compounds by means of PMR spectroscopy. Their acid-induced dehydration resulted in formation of ...

journal_title：Nucleic acids research

authors： Biernat J,Ciesiołka J,Górnicki P,Adamiak RW,Kryzosiak WJ,Wiewiórowski M

更新日期：1978-03-01 00:00:00

abstract：:Dimerization of HIV-1 genomic RNA is an essential step of the viral cycle, initiated at a conserved stem-loop structure which forms a 'kissing complex' involving loop-loop interactions (dimerization initiation site, DIS). A 19mer RNA oligonucleotide (DIS-19) has been synthesized which forms a stable symmetrical dimer ...

journal_title：Nucleic acids research

authors： Dardel F,Marquet R,Ehresmann C,Ehresmann B,Blanquet S

更新日期：1998-08-01 00:00:00

abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...

journal_title：Nucleic acids research

authors： Markham NR,Zuker M

更新日期：2005-07-01 00:00:00

abstract：:The primary rRNA transcript contains a large external transcribed spacer (ETS) approximately 4,000 nucleotides in length. We have used subcloned DNA probes derived from the 5' end of the ETS in conjunction with Northern blot analysis of murine nuclear RNA to examine processing of this region. In agreement with the res...

journal_title：Nucleic acids research

authors： Fetherston J,Werner E,Patterson R

更新日期：1984-09-25 00:00:00

abstract：:The MIPS Fusarium graminearum Genome Database (FGDB) is a comprehensive genome database on one of the most devastating fungal plant pathogens of wheat and barley. FGDB provides information on two gene sets independently derived by automated annotation of the F.graminearum genome sequence. A complete manually revised g...

journal_title：Nucleic acids research

authors： Güldener U,Mannhaupt G,Münsterkötter M,Haase D,Oesterheld M,Stümpflen V,Mewes HW,Adam G

更新日期：2006-01-01 00:00:00

abstract：:Homologous recombination provides a means for the in vivo construction of recombinant DNA molecules that may be problematic to assemble in vitro. We have investigated the efficiency of recombination within the Caenorhabditis elegans germ line as a function of the length of homology between recombining molecules. Our f...

journal_title：Nucleic acids research

authors： Kemp BJ,Hatzold J,Sternick LA,Cornman-Homonoff J,Whitaker JM,Tieu PJ,Lambie EJ

更新日期：2007-01-01 00:00:00

abstract：:The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130,000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77,000 Thalassiosira pseudonana ESTs from seven libraries...

journal_title：Nucleic acids research

authors： Maheswari U,Mock T,Armbrust EV,Bowler C

更新日期：2009-01-01 00:00:00

abstract：:The entire intervening sequence of Tetrahymena thermophila ribosomal DNA has been determined. It is 413 nucleotides long and has the same splice junctions as those in T. pigmentosa. There is 93% homology between the intervening sequences in the two species, and 100% homology between their adjacent 26S RNA coding regio...

journal_title：Nucleic acids research

authors： Kan NC,Gall JG

更新日期：1982-05-11 00:00:00

abstract：:The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. Th...

journal_title：Nucleic acids research

authors： Lackner KJ,Law SW,Brewer HB Jr

更新日期：1985-06-25 00:00:00

abstract：:Sequential chromatin immunoprecipitation (SeqChIP) is a procedure in which formaldehyde-crosslinked, protein-DNA complexes from living cells are subjected to two sequential immunoprecipitations with antibodies of different specificity. SeqChIP has been used to address, in a qualitative manner, whether two proteins can...

journal_title：Nucleic acids research

authors： Geisberg JV,Struhl K

更新日期：2004-11-01 00:00:00

abstract：:The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...

journal_title：Nucleic acids research

authors： Iioka H,Loiselle D,Haystead TA,Macara IG

更新日期：2011-04-01 00:00:00

abstract：:Pseudogenes are copies of genes that cannot produce a protein. They can be detected from disruptions to their apparent coding sequence, caused by frameshifts and premature stop codons. They are classed as either processed pseudogenes (made by reverse transcription from an mRNA) or duplicated pseudogenes, arising from ...

journal_title：Nucleic acids research

authors： Harrison PM,Milburn D,Zhang Z,Bertone P,Gerstein M

更新日期：2003-02-01 00:00:00

abstract：:RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established ...

journal_title：Nucleic acids research

authors： Liu Z,Zhao A,Chen L,Pape L

更新日期：1997-02-01 00:00:00

abstract：:Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...

journal_title：Nucleic acids research

authors： Yeoh LM,Goodman CD,Hall NE,van Dooren GG,McFadden GI,Ralph SA

更新日期：2015-05-19 00:00:00

abstract：:The 0.048502 megabase (Mb), primarily double-stranded DNA of bacteriophage lambda has single-stranded, complementary termini (cohesive ends) that undergo either spontaneous intramolecular joining to form open circular DNA or spontaneous intermolecular joining to form linear, end-to-end oligomeric DNAs (concatemers); c...

journal_title：Nucleic acids research

authors： Louie D,Serwer P

更新日期：1991-06-11 00:00:00

abstract：:Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the lactam functions in ...

journal_title：Nucleic acids research

authors： de Vroom E,Fidder A,Marugg JE,van der Marel GA,van Boom JH

更新日期：1986-07-25 00:00:00

abstract：:In vivo and in vitro RNA splicing experiments have demonstrated that the intron splicing machineries are not interchangeable in all organisms. These differences have prevented the efficient in vivo expression of monocot genes containing introns in dicot plants and the in vitro excision of some plant introns in HeLa ce...

journal_title：Nucleic acids research

authors： Hanley BA,Schuler MA

更新日期：1988-07-25 00:00:00

abstract：:Since its discovery in 1979, left-handed Z-DNA has evolved from an in vitro curiosity to a challenging DNA structure with crucial roles in gene expression, regulation and recombination. A fundamental question that has puzzled researchers for decades is how the transition from B-DNA, the prevalent right-handed form of ...

journal_title：Nucleic acids research

authors： Moradi M,Babin V,Roland C,Sagui C

更新日期：2013-01-07 00:00:00

abstract：:Two types of different repetitive DNA sequences called RepMP1 and RepMP2 were identified in the genome of Mycoplasma pneumoniae. The number of these repeated elements, their nucleotide sequence and their localization on a physical map of the M. pneumoniae genome were determined. The results show that RepMP1 appears at...

journal_title：Nucleic acids research

authors： Wenzel R,Herrmann R

更新日期：1988-09-12 00:00:00

abstract：:We report the extended database and data mining resources newly released in the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). H-InvDB is a comprehensive annotation resource of human genes and transcripts, and consists of two main views and six sub-databases. The latest release of H-InvDB (release 6...

journal_title：Nucleic acids research

authors： Yamasaki C,Murakami K,Takeda J,Sato Y,Noda A,Sakate R,Habara T,Nakaoka H,Todokoro F,Matsuya A,Imanishi T,Gojobori T

更新日期：2010-01-01 00:00:00

abstract：:No analogous nucleoside triphosphate was found which acts as well as ATP in binding to and supporting catalysis of leucyl-tRNA synthetase from Escherichia coli MRE 600. However, there are numerous nucleotides which are able to replace ATP, but with lower efficiency. The 6-amino group of the adenine ring and the 2'-hyd...

journal_title：Nucleic acids research

authors： Marutzky R,Flossdorf J,Kula MR

更新日期：1976-08-01 00:00:00

abstract：:During DNA double-strand break and replication fork repair by homologous recombination, the RAD51 recombinase catalyzes the DNA strand exchange reaction via a helical polymer assembled on single-stranded DNA, termed the presynaptic filament. Our published work has demonstrated a dual function of the SWI5-SFR1 complex ...

journal_title：Nucleic acids research

authors： Su GC,Yeh HY,Lin SW,Chung CI,Huang YS,Liu YC,Lyu PC,Chi P

更新日期：2016-07-27 00:00:00

abstract：:DNA polymerases alpha and delta are essential enzymes believed to play critical roles in initiation and replication of chromosome DNA. In this study, we show that the genes for Schizosaccharomyces pombe (S.pombe) DNA polymerase alpha and delta (pol alpha+ and pol delta+) are essential for cell viability. Disruption of...

journal_title：Nucleic acids research

authors： Francesconi S,Park H,Wang TS

更新日期：1993-08-11 00:00:00

abstract：:The complete nucleotide sequence of the mitochondrial DNA of the amphioxus Branchiostoma lanceolatum has been determined. This mitochondrial genome is small (15 076 bp) because of the short size of the two rRNA genes and the tRNA genes. In addition, this genome contains a very short non-coding region (57 bp) with no s...

journal_title：Nucleic acids research

authors： Spruyt N,Delarbre C,Gachelin G,Laudet V

更新日期：1998-07-01 00:00:00

abstract：:The webPDBinder (http://pdbinder.bio.uniroma2.it/PDBinder) is a web server for the identification of small ligand-binding sites in a protein structure. webPDBinder searches a protein structure against a library of known binding sites and a collection of control non-binding pockets. The number of similarities identifie...

journal_title：Nucleic acids research

authors： Bianchi V,Mangone I,Ferrè F,Helmer-Citterich M,Ausiello G

更新日期：2013-07-01 00:00:00

abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...

journal_title：Nucleic acids research

authors： Turro E,Lewin A,Rose A,Dallman MJ,Richardson S

更新日期：2010-01-01 00:00:00

abstract：:A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Strongylocentrotus purpuratus has been characterized, and shown to contain the sequence features which indicate that ...

journal_title：Nucleic acids research

authors： Nisson PE,Hickey RJ,Boshar MF,Crain WR Jr

更新日期：1988-02-25 00:00:00