SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing.

abstract：:The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silence...

journal_title：Nucleic acids research

authors： Zhu X,Zhang Y,Bjornsdottir G,Liu Z,Quan A,Costanzo M,Dávila López M,Westholm JO,Ronne H,Boone C,Gustafsson CM,Myers LC

更新日期：2011-10-01 00:00:00

abstract：:Gene losses provide an insightful route for studying the morphological and physiological adaptations of species, but their discovery is challenging. Existing genome annotation tools focus on annotating intact genes and do not attempt to distinguish nonfunctional genes from genes missing annotation due to sequencing an...

journal_title：Nucleic acids research

authors： Turakhia Y,Chen HI,Marcovitz A,Bejerano G

更新日期：2020-09-18 00:00:00

abstract：:Here we describe the application of a novel combinatorial method, restriction endonuclease protection selection and amplification (REPSA), to identification of a consensus DNA binding site for the TATA binding subunit (hTBP) of the human general transcription factor TFIID. Unlike most combinatorial methods, REPSA is b...

journal_title：Nucleic acids research

authors： Hardenbol P,Wang JC,Van Dyke MW

更新日期：1997-08-15 00:00:00

abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...

journal_title：Nucleic acids research

authors： Trilling M,Bellora N,Rutkowski AJ,de Graaf M,Dickinson P,Robertson K,Prazeres da Costa O,Ghazal P,Friedel CC,Albà MM,Dölken L

更新日期：2013-09-01 00:00:00

abstract：:TFIIIC is a RNA polymerase (pol) III-specific DNA-binding factor that is required for transcription of tRNA and 5S rRNA genes. Active human TFIIIC consists of five subunits. However, an inactive form has also been isolated that lacks one of the five subunits, called TFIIIC110. A model was proposed in which pol III tra...

journal_title：Nucleic acids research

authors： Innes F,Ramsbottom B,White RJ

更新日期：2006-07-05 00:00:00

abstract：:We present RADAR--a rigorously annotated database of A-to-I RNA editing (available at http://RNAedit.com). The identification of A-to-I RNA editing sites has been dramatically accelerated in the past few years by high-throughput RNA sequencing studies. RADAR includes a comprehensive collection of A-to-I RNA editing si...

journal_title：Nucleic acids research

authors： Ramaswami G,Li JB

更新日期：2014-01-01 00:00:00

abstract：:PCAF and hGCN5 are distinct human genes that encode proteins related to the yeast histone acetyltransferase and transcriptional adapter GCN5. The PCAF protein shares extensive similarity with the 439 amino acids of yGCN5, but it has an approximately 350 amino acid N-terminal extension that interacts with the transcrip...

journal_title：Nucleic acids research

authors： Smith ER,Belote JM,Schiltz RL,Yang XJ,Moore PA,Berger SL,Nakatani Y,Allis CD

更新日期：1998-06-15 00:00:00

abstract：:During mitosis the chromatin undergoes dramatic architectural changes with the halting of the transcriptional processes and evacuation of nearly all transcription associated machinery from genes and promoters. Molecular bookmarking of genes during mitosis is a mechanism of faithfully transmitting cell-specific transcr...

journal_title：Nucleic acids research

authors： Arora M,Packard CZ,Banerjee T,Parvin JD

更新日期：2016-03-18 00:00:00

abstract：:An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...

journal_title：Nucleic acids research

authors： Ponelies N,Stein N,Weber G

更新日期：1997-09-01 00:00:00

abstract：:In mammalian cells, DNA damage induces robust changes in gene expression and these changes contribute to the proper execution of cellular responses to DNA damage, including DNA repair, cell cycle arrest and apoptosis. The transcription factor E2F-1 has been suggested to play a key role in the regulation of cell cycle-...

journal_title：Nucleic acids research

authors： Höfferer M,Wirbelauer C,Humar B,Krek W

更新日期：1999-01-15 00:00:00

abstract：:Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells ...

journal_title：Nucleic acids research

authors： McLennan AG,Prescott M

更新日期：1984-02-10 00:00:00

abstract：:We have examined the absorption and circular dichroism spectra of intact Penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded RNA. The absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded RNA and free viral protein, ind...

journal_title：Nucleic acids research

authors： Edmondson SP,Gray DM

更新日期：1983-01-11 00:00:00

abstract：:Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...

journal_title：Nucleic acids research

authors： Jegga AG,Gupta A,Gowrisankar S,Deshmukh MA,Connolly S,Finley K,Aronow BJ

更新日期：2005-07-01 00:00:00

abstract：:Engineered derivatives of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) possessing a unique restriction site provide a source of viral DNA that can be linearized by digestion with a specific endonuclease. Circular or linearized DNA from two such viruses were compared in terms of thei...

journal_title：Nucleic acids research

authors： Kitts PA,Ayres MD,Possee RD

更新日期：1990-10-11 00:00:00

abstract：:We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizi...

journal_title：Nucleic acids research

authors： Lawrance SK,Das HK,Pan J,Weissman SM

更新日期：1985-10-25 00:00:00

abstract：:The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...

journal_title：Nucleic acids research

authors： Jones WR,Stone MP

更新日期：1998-02-15 00:00:00

abstract：:We have previously reported the isolation of mammalian cell lines expressing the 3-methyladenine DNA glycosylase I (tag) gene from E. coli. These cells are 2-5 fold more resistant to the toxic effects of methylating agents than normal cells (15). Kinetic measurements of 3-methyladenine removal from the genome in situ ...

journal_title：Nucleic acids research

authors： Klungland A,Bjørås M,Hoff E,Seeberg E

更新日期：1994-05-11 00:00:00

abstract：:The archaeal exosome is a phosphorolytic 3'-5' exoribonuclease complex. In a reverse reaction it synthesizes A-rich RNA tails. Its RNA-binding cap comprises the eukaryotic orthologs Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. In Sulfolobus solfataricus DnaG and Rrp4 but not Csl4 show preference f...

journal_title：Nucleic acids research

authors： Hou L,Klug G,Evguenieva-Hackenberg E

更新日期：2014-11-10 00:00:00

abstract：:Novel techniques for high-throughput steady-state metabolomic profiling yield information about changes of nearly thousands of metabolites. Such metabolomic profiles, when analyzed together with transcriptional profiles, can reveal novel insights about underlying biological processes. While a number of conceptual appr...

journal_title：Nucleic acids research

authors： Sergushichev AA,Loboda AA,Jha AK,Vincent EE,Driggers EM,Jones RG,Pearce EJ,Artyomov MN

更新日期：2016-07-08 00:00:00

abstract：:Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and speci...

journal_title：Nucleic acids research

authors： Kim H,Kang N,An K,Kim D,Koo J,Kim MS

更新日期：2017-01-04 00:00:00

abstract：:The 5' and 3' untranslated regions of eukaryotic mRNAs (UTRs) play crucial roles in the post-transcriptional regulation of gene expression through the modulation of nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and message stability. UTRdb is a curated database of 5' and 3' untran...

journal_title：Nucleic acids research

authors： Grillo G,Turi A,Licciulli F,Mignone F,Liuni S,Banfi S,Gennarino VA,Horner DS,Pavesi G,Picardi E,Pesole G

更新日期：2010-01-01 00:00:00

abstract：:A prototype of a novel class of DNA alkylating agents, which combines the DNA crosslinking moiety chlorambucil (Chl) with a sequence-selective hairpin pyrrole-imidazole polyamide ImPy-beta-ImPy-gamma-ImPy-beta-Dp (polyamide 1), was evaluated for its ability to damage DNA and induce biological responses. Polyamide 1-Ch...

journal_title：Nucleic acids research

authors： Wang YD,Dziegielewski J,Wurtz NR,Dziegielewska B,Dervan PB,Beerman TA

更新日期：2003-02-15 00:00:00

abstract：:It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-le...

journal_title：Nucleic acids research

authors： Kataoka N,Ohno M,Moda I,Shimura Y

更新日期：1995-09-25 00:00:00

abstract：:The multi-protein complex WRAD, formed by WDR5, RbBP5, Ash2L and Dpy30, binds to the MLL SET domain to stabilize the catalytically active conformation required for histone H3K4 methylation. In addition, the WRAD complex contributes to the targeting of the activated complex to specific sites on chromatin. RbBP5 is cent...

journal_title：Nucleic acids research

authors： Mittal A,Hobor F,Zhang Y,Martin SR,Gamblin SJ,Ramos A,Wilson JR

更新日期：2018-04-20 00:00:00

abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...

journal_title：Nucleic acids research

authors： Liu WM,Chu WM,Choudary PV,Schmid CW

更新日期：1995-05-25 00:00:00

abstract：:The rates of acid-catalyzed hydrolysis of a hypermodified nucleoside, wyosine and its 5'-monophosphate were determined at various pH, temperature and buffer concentrations. The results show that despite distinct differences in structure and the glycosyl bond stability, the hydrolysis of wyosine proceeds via cleavage o...

journal_title：Nucleic acids research

authors： Golankiewicz B,Zielonacka-Lis E,Folkman W

更新日期：1985-04-11 00:00:00

abstract：:Analysis of haplotypes is an important tool in population genetics, familial heredity and gene mapping. Determination of haplotypes of multiple single nucleotide polymorphisms (SNPs) or other simple mutations is time consuming and expensive when analyzing large populations, and often requires the help of computational...

journal_title：Nucleic acids research

authors： Eitan Y,Kashi Y

更新日期：2002-06-15 00:00:00

abstract：:MAGIA(2) (http://gencomp.bio.unipd.it/magia2) is an update, extension and evolution of the MAGIA web tool. It is dedicated to the integrated analysis of in silico target prediction, microRNA (miRNA) and gene expression data for the reconstruction of post-transcriptional regulatory networks. miRNAs are fundamental post...

journal_title：Nucleic acids research

authors： Bisognin A,Sales G,Coppe A,Bortoluzzi S,Romualdi C

更新日期：2012-07-01 00:00:00

abstract：:Transcriptomics (at the level of single cells, tissues and/or whole organisms) underpins many fields of biomedical science, from understanding the basic cellular function in model organisms, to the elucidation of the biological events that govern the development and progression of human diseases, and the exploration o...

journal_title：Nucleic acids research

authors： Cantacessi C,Jex AR,Hall RS,Young ND,Campbell BE,Joachim A,Nolan MJ,Abubucker S,Sternberg PW,Ranganathan S,Mitreva M,Gasser RB

更新日期：2010-09-01 00:00:00

abstract：:Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side o...

journal_title：Nucleic acids research

authors： Cochet M,Perrin F,Gannon F,Krust A,Chambon P,McKnight GS,Lee DC,Mayo KE,Palmiter R

更新日期：1979-06-11 00:00:00