Histone modifications influence mediator interactions with chromatin.

abstract：:Plasmodium falciparum origin recognition complex 1 (ORC1) protein has been implicated in DNA replication and silencing var gene family. However, the mechanism and the domain structure of ORC1 related to the regulation of var gene family are unknown. Here we show that the unique N-terminus of PfORC1 (PfORC1N(1-238)) is...

journal_title：Nucleic acids research

authors： Deshmukh AS,Srivastava S,Herrmann S,Gupta A,Mitra P,Gilberger TW,Dhar SK

更新日期：2012-07-01 00:00:00

abstract：:Treatment of the ciliated protozoan Tetrahymena thermophila with 8-methoxypsoralen combined with long wavelength ultraviolet irradiation (UVA, lambda approximately 360 nm) resulted in a dose dependent equal inhibition of the synthesis of rRNA, 5.8S, 5S and tRNA. Similar results were obtained with 3-carbethoxy-8-methox...

journal_title：Nucleic acids research

authors： Nielsen PE

更新日期：1987-02-11 00:00:00

abstract：:Translation initiation in eukaryotes is mediated by assembly of the eIF4F complex over the m(7)GTP cap structure at the 5'-end of mRNAs. This requires an interaction between eIF4E and eIF4G, two eIF4F subunits. The Leishmania orthologs of eIF4E are structurally diverged from their higher eukaryote counterparts, since ...

journal_title：Nucleic acids research

authors： Yoffe Y,Léger M,Zinoviev A,Zuberek J,Darzynkiewicz E,Wagner G,Shapira M

更新日期：2009-06-01 00:00:00

abstract：:Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexib...

journal_title：Nucleic acids research

authors： Fritzsche H,Rupprecht A,Richter M

更新日期：1984-12-11 00:00:00

abstract：:Eukaryotic cellular mRNAs contain a cap at their 5'-ends, but some viral and cellular mRNAs bypass the cap-dependent mechanism of translation initiation in favor of internal entry of ribosomes at specific RNA sequences. Cap-dependent initiation requires intact initiation factor eIF4G (formerly eIF-4gamma, eIF-4Fgamma ...

journal_title：Nucleic acids research

authors： Keiper BD,Rhoads RE

更新日期：1997-01-15 00:00:00

abstract：:In addition to maintaining the GenBank® nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data made available through the NCBI Web site. NCBI resources include Entrez, the Entrez Programming Ut...

journal_title：Nucleic acids research

authors： Sayers EW,Barrett T,Benson DA,Bolton E,Bryant SH,Canese K,Chetvernin V,Church DM,DiCuccio M,Federhen S,Feolo M,Fingerman IM,Geer LY,Helmberg W,Kapustin Y,Landsman D,Lipman DJ,Lu Z,Madden TL,Madej T,Maglott DR,Ma

更新日期：2011-01-01 00:00:00

abstract：:Homozygosity for a natural deletion variant of the HIV-coreceptor molecule CCR5, CCR5Δ32, confers resistance toward HIV infection. Allogeneic stem cell transplantation from a CCR5Δ32-homozygous donor has resulted in the first cure from HIV ('Berlin patient'). Based thereon, genetic disruption of CCR5 using designer nu...

journal_title：Nucleic acids research

authors： Mock U,Machowicz R,Hauber I,Horn S,Abramowski P,Berdien B,Hauber J,Fehse B

更新日期：2015-06-23 00:00:00

abstract：:We show that using low denaturation temperatures (80-88 degrees C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the PCR meltin...

journal_title：Nucleic acids research

authors： Masny A,Płucienniczak A

更新日期：2003-09-15 00:00:00

abstract：:A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several s...

journal_title：Nucleic acids research

authors： Koski RA,Allison DS,Worthington M,Hall BD

更新日期：1982-12-20 00:00:00

abstract：:Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length ...

journal_title：Nucleic acids research

authors： Miyamoto-Sato E,Nemoto N,Kobayashi K,Yanagawa H

更新日期：2000-03-01 00:00:00

abstract：:Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overo...

journal_title：Nucleic acids research

authors： Eiamphungporn W,Soonsanga S,Lee JW,Helmann JD

更新日期：2009-03-01 00:00:00

abstract：:Programmed -1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slip...

journal_title：Nucleic acids research

authors： Lin Z,Gilbert RJ,Brierley I

更新日期：2012-09-01 00:00:00

abstract：:We report the isolation of a novel human POU domain encoding gene named RDC-1. The POU domain of the RDC-1 encoded protein is highly related to the POU domain potentially encoded by the rat brain-3 sequence and to that of the Drosophila I-POU protein; outside of the POU region, RDC-1 is unrelated to any previously cha...

journal_title：Nucleic acids research

authors： Collum RG,Fisher PE,Datta M,Mellis S,Thiele C,Huebner K,Croce CM,Israel MA,Theil T,Moroy T

更新日期：1992-09-25 00:00:00

abstract：:The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Archaea and Bacteria. CRISPR loci integrate short DNA sequences from invading genetic elements that provide small RNA-mediated interference in subsequent exposure to matching nucleic acids. In Streptococcus thermophilus, it was pr...

journal_title：Nucleic acids research

authors： Sapranauskas R,Gasiunas G,Fremaux C,Barrangou R,Horvath P,Siksnys V

更新日期：2011-11-01 00:00:00

abstract：:In this study, we present the DNA-Binding Site Identifier (DBSI), a new structure-based method for predicting protein interaction sites for DNA binding. DBSI was trained and validated on a data set of 263 proteins (TRAIN-263), tested on an independent set of protein-DNA complexes (TEST-206) and data sets of 29 unbound...

journal_title：Nucleic acids research

authors： Zhu X,Ericksen SS,Mitchell JC

更新日期：2013-09-01 00:00:00

abstract：:Many complex viruses package their genomes into empty protein shells and bacteriophages of the Cystoviridae family provide some of the simplest models for this. The cystoviral hexameric NTPase, P4, uses chemical energy to translocate single-stranded RNA genomic precursors into the procapsid. We previously dissected th...

journal_title：Nucleic acids research

authors： El Omari K,Meier C,Kainov D,Sutton G,Grimes JM,Poranen MM,Bamford DH,Tuma R,Stuart DI,Mancini EJ

更新日期：2013-11-01 00:00:00

abstract：:Poly(ADP-ribosyl)ation is involved in numerous bio-logical processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading enzyme PAR glycohydrolase (PARG), controlling the cell fate decision between life and death in re...

journal_title：Nucleic acids research

authors： Illuzzi G,Fouquerel E,Amé JC,Noll A,Rehmet K,Nasheuer HP,Dantzer F,Schreiber V

更新日期：2014-07-01 00:00:00

abstract：:The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce pADH06C. The yeast strains SX46A, 7799-4B and VV-6 are deficient in endogenous O6-alkylguanine-DNA-alkyltransferase and...

journal_title：Nucleic acids research

authors： Brozmanova J,Kleibl K,Vlckova V,Skorvaga M,Cernakova L,Margison GP

更新日期：1990-01-25 00:00:00

abstract：:2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical s...

journal_title：Nucleic acids research

authors： Schmidt S,Pein CD,Fritz HJ,Cech D

更新日期：1992-05-25 00:00:00

abstract：:Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both ...

journal_title：Nucleic acids research

authors： Kasai H,Shindo-Okada N,Noguchi S,Nishimura S

更新日期：1979-09-11 00:00:00

abstract：:We have demonstrated that the DNA sequence between two triplex-forming polypurine.polypyrimidine (Pu.Py) tracts was protected from DNA modifying enzymes upon formation of triplex DNA structures with an oligodeoxyribonucleotide in which two triplex-forming Pu or Py tracts were placed at the termini (triplex-bridge form...

journal_title：Nucleic acids research

authors： Kiyama R,Oishi M

更新日期：1995-02-11 00:00:00

abstract：:We report for the first time an analysis of the ATPase activity of human DNA topoisomerase (topo) IIbeta. We show that topo IIbeta is a DNA-dependent ATPase that appears to fit Michaelis-Menten kinetics. The ATPase activity is stimulated 44-fold by DNA. The k(cat) for ATP hydrolysis by human DNA topo IIbeta in the pre...

journal_title：Nucleic acids research

authors： West KL,Turnbull RM,Willmore E,Lakey JH,Austin CA

更新日期：2002-12-15 00:00:00

abstract：:Partial acid cleavage, comparative HPLC tryptic peptide mapping and amino acid sequencing of the C1 and C2 proteins of HeLa heterogeneous nuclear ribonucleoprotein (hnRNP) particles demonstrate that proteins C1 and C2 differ in primary structure by the presence of a 13 amino acid insert sequence in C2. This C2 insert ...

journal_title：Nucleic acids research

authors： Merrill BM,Barnett SF,LeStourgeon WM,Williams KR

更新日期：1989-11-11 00:00:00

abstract：:Saccharomyces cerevisiae chl1 mutants have a significant increase in the rate of chromosome missegregation. CHL1 encodes a 99 kDa predicted protein with an ATP binding site consensus, a putative helix-turn-helix DNA binding motif, and homology to helicases. Using site-directed mutagenesis, I show that mutations that a...

journal_title：Nucleic acids research

authors： L Holloway S

更新日期：2000-08-15 00:00:00

abstract：:NONO, SFPQ and PSPC1 make up a family of proteins with diverse roles in transcription, RNA processing and DNA double-strand break (DSB) repair. To understand long-term effects of loss of NONO, we characterized murine embryonic fibroblasts (MEFs) from knockout mice. In the absence of genotoxic stress, wild-type and mut...

journal_title：Nucleic acids research

authors： Li S,Li Z,Shu FJ,Xiong H,Phillips AC,Dynan WS

更新日期：2014-09-01 00:00:00

abstract：:Cell differentiation is a central process in development and in cancer growth and dissemination. OCT4 (POU5F1) and NANOG are essential for cell stemness and pluripotency; yet, the mechanisms that regulate their expression remain largely unknown. Repetitive elements account for almost half of the Human Genome; still, t...

journal_title：Nucleic acids research

authors： Morales-Hernández A,González-Rico FJ,Román AC,Rico-Leo E,Alvarez-Barrientos A,Sánchez L,Macia Á,Heras SR,García-Pérez JL,Merino JM,Fernández-Salguero PM

更新日期：2016-06-02 00:00:00

abstract：:Monocrotaline is a pyrrolizidine alkaloid known to cause toxicity in humans and animals. Its mechanism of biological action is still unclear although DNA crosslinking has been suggested to a play a role in its activity. In this study we found that an active metabolite of monocrotaline, dehydromonocrotaline (DHM), alky...

journal_title：Nucleic acids research

authors： Pereira TN,Webb RI,Reilly PE,Seawright AA,Prakash AS

更新日期：1998-12-01 00:00:00

abstract：:Restriction fragments containing established curved regions, such as the pBR322 tn3 region, the phage lambda attP region and the SV40 T-antigen and terminus of replication regions, exhibit systematically retarded elution upon anion exchange based HPLC. Using this method, we were able to detect readily other SV40 curve...

journal_title：Nucleic acids research

authors： Fack F,Sarantoglou V

更新日期：1991-08-11 00:00:00

abstract：:Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...

journal_title：Nucleic acids research

authors： Duchi D,Gryte K,Robb NC,Morichaud Z,Sheppard C,Brodolin K,Wigneshweraraj S,Kapanidis AN

更新日期：2018-01-25 00:00:00

abstract：:The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...

journal_title：Nucleic acids research

authors： Bayona-Bafaluy MP,Manfredi G,Moraes CT

更新日期：2003-08-15 00:00:00