Long internal inverted repeat in a yeast viral double-stranded RNA.

abstract：:A cDNA clone which expresses a protein that cross-reacts immunologically with the human C1 and C2 hnRNP core proteins has been isolated. The clone was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive ...

journal_title：Nucleic acids research

authors： Lahiri DK,Thomas JO

更新日期：1986-05-27 00:00:00

abstract：:Long terminal repeat (LTR)-retrotransposons comprise a significant portion of the rice genome. Their complete characterization is thus necessary if the sequenced genome is to be annotated correctly. In addition, because LTR-retrotransposons can influence the expression of neighboring genes, the complete identification...

journal_title：Nucleic acids research

authors： Chaparro C,Guyot R,Zuccolo A,Piégu B,Panaud O

更新日期：2007-01-01 00:00:00

abstract：:The thermal stability of the Tn10 encoded tetracycline resistance (TET) gene control region is investigated by melting studies using purified DNA restriction fragments containing various amounts of flanking sequences. In order to study the thermodynamic properties of this control region under conditions, where enough ...

journal_title：Nucleic acids research

authors： Hillen W,Unger B

更新日期：1982-04-24 00:00:00

abstract：:The role of thymine dimerization for previously reported U.V. induced conformational changes has been proved using acetophenone as sensitizer for the specific thymine dimerization upon irradiation at 313 nm. CD results demonstrate that formation of pyrimidine dimers cause typical conformational changes of the DNA B he...

journal_title：Nucleic acids research

authors： Lang H

更新日期：1975-02-01 00:00:00

abstract：:Heptadecaoligodeoxyribonucleotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these basemodified ol...

journal_title：Nucleic acids research

authors： Lin PK,Brown DM

更新日期：1992-10-11 00:00:00

abstract：:8-Oxo-7,8,-dihydro-2'-deoxyguanosine triphosphate (8-oxo-dGTP) is a major product of oxidative damage in the nucleotide pool. It is capable of mispairing with adenosine (dA), resulting in futile, mutagenic cycles of base excision repair. Therefore, it is critical that DNA polymerases discriminate against 8-oxo-dGTP at...

journal_title：Nucleic acids research

authors： Burak MJ,Guja KE,Garcia-Diaz M

更新日期：2015-09-18 00:00:00

abstract：:Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires sequence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding of the duplex prior to subsequent leading strand DNA synthesis. Nicking is performed by a replication-initiation protein (Rep)...

journal_title：Nucleic acids research

authors： Pastrana CL,Carrasco C,Akhtar P,Leuba SH,Khan SA,Moreno-Herrero F

更新日期：2016-10-14 00:00:00

abstract：:Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional charact...

journal_title：Nucleic acids research

authors： Benders GA,Noskov VN,Denisova EA,Lartigue C,Gibson DG,Assad-Garcia N,Chuang RY,Carrera W,Moodie M,Algire MA,Phan Q,Alperovich N,Vashee S,Merryman C,Venter JC,Smith HO,Glass JI,Hutchison CA 3rd

更新日期：2010-05-01 00:00:00

abstract：:The 3' terminal nucleotide sequence of two clones containing DNA complementary to mu chain mRNA of IgM-secreting cells has been determined. The sequence shows a termination codon (UGA) adjacent to the terminal tyrosine codon for the secreted protein and a 3' non-coding region of at least 106 bases. The primary transla...

journal_title：Nucleic acids research

authors： Matthyssens G,Rabbitts TH

更新日期：1980-02-25 00:00:00

abstract：:Period2 (Per2) is an essential component of the mammalian clock mechanism and robust circadian expression of Per2 is essential for the maintenance of circadian rhythms. Although recent studies have shown that the circadian E2 enhancer (a non-canonical E-box) accounts for most of the circadian transcriptional drive of ...

journal_title：Nucleic acids research

authors： Ohno T,Onishi Y,Ishida N

更新日期：2007-01-01 00:00:00

abstract：:A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removin...

journal_title：Nucleic acids research

authors： Kalmár T,Bachrati CZ,Marcsik A,Raskó I

更新日期：2000-06-15 00:00:00

abstract：:The MED1/TRAP220 subunit of the Mediator plays a key role in facilitating ligand-dependent interactions of this multisubunit coactivator complex with nuclear receptors through their ligand binding domains. The isolated MED1/TRAP220 protein previously was shown to interact with glucocorticoid receptor (GR) in a ligand-...

journal_title：Nucleic acids research

authors： Chen W,Roeder RG

更新日期：2007-01-01 00:00:00

abstract：:The Class 3 aldehyde dehydrogenase gene (ALDH-3) is differentially expressed. Expression is either constitutive or xenobiotic inducible via an aromatic hydrocarbon (Ah) receptor-mediated pathway, depending upon the tissue. A series of studies were performed to examine the regulation of rat ALDH-3 basal expression. DNa...

journal_title：Nucleic acids research

authors： Xie YQ,Takimoto K,Pitot HC,Miskimins WK,Lindahl R

更新日期：1996-11-01 00:00:00

abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...

journal_title：Nucleic acids research

authors： Nagashima T,Matsuda H,Silva DG,Petrovsky N,Konagaya A,Schönbach C,Kasukawa T,Arakawa T,Carninci P,Kawai J,Hayashizaki Y,RIKEN GER Group.,Genome Science Laoratory Members.

更新日期：2004-01-01 00:00:00

abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...

journal_title：Nucleic acids research

authors： Hummel S,Meyerhof W,Korge E,Knöchel W

更新日期：1984-06-25 00:00:00

abstract：:Small regulatory RNAs (sRNAs) from bacterial chromosomes became the focus of research over the past five years. However, relatively little is known in terms of structural requirements, kinetics of interaction with their targets and degradation in contrast to well-studied plasmid-encoded antisense RNAs. Here, we presen...

journal_title：Nucleic acids research

authors： Heidrich N,Moll I,Brantl S

更新日期：2007-01-01 00:00:00

abstract：:Successful development of biological databases requires accommodation of the burgeoning amounts of data from high-throughput genomics pipelines. As the volume of curated data in Animal QTLdb (https://www.animalgenome.org/QTLdb) increases exponentially, the resulting challenges must be met with rapid infrastructure dev...

journal_title：Nucleic acids research

authors： Hu ZL,Park CA,Reecy JM

更新日期：2019-01-08 00:00:00

abstract：:A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces ...

journal_title：Nucleic acids research

authors： Yamashita A,Takayama T,Iwata R,Yamamoto M

更新日期：2013-11-01 00:00:00

abstract：:Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mu...

journal_title：Nucleic acids research

authors： Kohler SW,Provost GS,Kretz PL,Dycaico MJ,Sorge JA,Short JM

更新日期：1990-05-25 00:00:00

abstract：:Abasic substitutions in the non-template strand and promoter sequence changes were made to assess the roles of various promoter features in sigma70 holoenzyme interactions with fork junction probes. Removal of -10 element non-template single strand bases, leaving the phosphodiester backbone intact, did not interfere w...

journal_title：Nucleic acids research

authors： Fenton MS,Gralla JD

更新日期：2003-06-01 00:00:00

abstract：:PRINTS is a database of protein family 'fingerprints' offering a diagnostic resource for newly-determined sequences. By contrast with PROSITE, which uses single consensus expressions to characterise particular families, PRINTS exploits groups of motifs to build characteristic signatures. These signatures offer improve...

journal_title：Nucleic acids research

authors： Attwood TK,Beck ME,Flower DR,Scordis P,Selley JN

更新日期：1998-01-01 00:00:00

abstract：:RNA structural complexity and flexibility present a challenge for computational modeling efforts. Experimental information and bioinformatics data can be used as restraints to improve the accuracy of RNA tertiary structure prediction. Regarding utilization of restraints, the fundamental questions are: (i) What is the ...

journal_title：Nucleic acids research

authors： Wang J,Williams B,Chirasani VR,Krokhotin A,Das R,Dokholyan NV

更新日期：2019-06-20 00:00:00

abstract：:We recently compared the efficiency of six picornaviral internal ribosome entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their ability to drive internal initiation of translationin vitro. Here we present the results of a similar comparison performed in six different cultured cell lines infected with ...

journal_title：Nucleic acids research

authors： Borman AM,Le Mercier P,Girard M,Kean KM

更新日期：1997-03-01 00:00:00

abstract：:A 12 bp long GCN4-binding, self-complementary duplex DNA d(CATGACGTCATG)(2) has been investigated by NMR spectroscopy to study the structure and dynamics of the molecule in aqueous solution. The NMR structure of the DNA obtained using simulated annealing and iterative relaxation matrix calculations compares quite clos...

journal_title：Nucleic acids research

authors： Khandelwal P,Panchal SC,Radha PK,Hosur RV

更新日期：2001-01-15 00:00:00

abstract：:The Epstein-Barr virus gene BRLF1 encodes the transcription factor R, which is a sequence-specific DNA-binding protein important for the switch from latency to a productive cycle. We have defined a repertoire of specific R-binding sites using a GST-R fusion protein and a pool of 23 bp random DNA sequences. The R-bound...

journal_title：Nucleic acids research

authors： Gruffat H,Sergeant A

更新日期：1994-04-11 00:00:00

abstract：:To understand the epigenetic regulation of transcriptional response of macrophages during early-stage M. tuberculosis (Mtb) infection, we performed ChIPseq analysis of H3K4 monomethylation (H3K4me1), a marker of poised or active enhancers. De novo H3K4me1 peaks in infected cells were associated with genes implicated i...

journal_title：Nucleic acids research

authors： Bouttier M,Laperriere D,Memari B,Mangiapane J,Fiore A,Mitchell E,Verway M,Behr MA,Sladek R,Barreiro LB,Mader S,White JH

更新日期：2016-12-15 00:00:00

abstract：:Three protein isoforms (74, 54 and 42 kDa) are expressed from repressor gene c in the Streptomyces temperate bacteriophage phiC31. Because expression of the two smaller isoforms, 54 and 42 kDa, is sufficient for superinfection immunity, the interaction between these isoforms was studied. The native 42 kDa repressor (N...

journal_title：Nucleic acids research

authors： Wilson SE,Smith MC

更新日期：1998-05-15 00:00:00

abstract：:Plant specialized metabolites are chemically highly diverse, play key roles in host-microbe interactions, have important nutritional value in crops and are frequently applied as medicines. It has recently become clear that plant biosynthetic pathway-encoding genes are sometimes densely clustered in specific genomic lo...

journal_title：Nucleic acids research

authors： Kautsar SA,Suarez Duran HG,Blin K,Osbourn A,Medema MH

更新日期：2017-07-03 00:00:00

abstract：:The enterovirus 71 (EV71) 3Dpol is an RNA-dependent RNA polymerase (RdRP) that plays the central role in the viral genome replication, and is an important target in antiviral studies. Here, we report a crystal structure of EV71 3Dpol elongation complex (EC) at 1.8 Å resolution. The structure reveals that the 5'-end gu...

journal_title：Nucleic acids research

authors： Shi W,Ye HQ,Deng CL,Li R,Zhang B,Gong P

更新日期：2020-02-20 00:00:00

abstract：:This work describes the organization, at the nucleotide sequence level, of genes flanking the junctions of the large single copy regions and the inverted repeats of Spinacia oleracea (spinach) and Nicotiana debneyi chloroplast DNAs. In both genomes, trnH1, the gene for tRNA-His(GUG) is located at the extremity of the ...

journal_title：Nucleic acids research

authors： Zurawski G,Bottomley W,Whitfeld PR

更新日期：1984-08-24 00:00:00