Abstract:
:The base excision repair DNA glycosylase MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro-8-oxo-guanine (8-oxoG), thereby preventing G:C to T:A mutations. Biallelic germline mutations in the human MYH gene predispose individuals to multiple colorectal adenomas and carcinoma. We have recently demonstrated that hMYH interacts with the mismatch repair protein hMSH6, and that the hMSH2/hMSH6 (hMutSalpha) heterodimer stimulates hMYH activity. Here, we characterize the functional effect of two missense mutations (R227W and V232F) associated with hMYH polyposis that lie within, or adjacent to, the putative hMSH6 binding domain. Neither missense mutation affects the physical interaction between hMYH and hMSH6. However, hMYH(R227W) has a severe defect in A/8-oxoG binding and glycosylase activities, while hMYH(V232F) has reduced A/8-oxoG binding and glycosylase activities. The glycosylase activity of the V232F mutant can be partially stimulated by hMutSalpha but cannot be restored to the wild-type level. Both mutants also fail to complement mutY-deficiency in Escherichia coli. These data define the pathogenic mechanisms underlying two further hMYH polyposis-associated mutations.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bai H,Jones S,Guan X,Wilson TM,Sampson JR,Cheadle JP,Lu ALdoi
10.1093/nar/gki209keywords:
subject
Has Abstractpub_date
2005-01-26 00:00:00pages
597-604issue
2eissn
0305-1048issn
1362-4962pii
33/2/597journal_volume
33pub_type
杂志文章abstract::DISCOVER1 (DIStribution COunter VERsion 1) is a new program that can identify DNA motifs occurring with a high deviation from the expected frequency. The program generates families of patterns, each family having a common set of defined bases. Undefined bases are inserted amongst the defined bases in different ways, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.22.5152
更新日期:1993-11-11 00:00:00
abstract::The binding of transcription factors to specific regulatory sequence elements is a primary mechanism for controlling gene transcription. Eukaryotic genes are often regulated by several transcription factors whose binding sites are tightly clustered and form cis-regulatory modules. In this paper, we present a web serve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh385
更新日期:2004-07-01 00:00:00
abstract::Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.2.e2
更新日期:2000-01-15 00:00:00
abstract::The MyHits web site (http://myhits.isb-sib.ch) is an integrated service dedicated to the analysis of protein sequences. Since its first description in 2004, both the user interface and the back end of the server were improved. A number of tools (e.g. MAFFT, Jacop, Dotlet, Jalview, ESTScan) were added or updated to imp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm352
更新日期:2007-07-01 00:00:00
abstract::High throughput sequencing (HTS) platforms generate unprecedented amounts of data that introduce challenges for processing and downstream analysis. While tools that report the 'best' mapping location of each read provide a fast way to process HTS data, they are not suitable for many types of downstream analysis such a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku370
更新日期:2014-07-01 00:00:00
abstract::The viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz313
更新日期:2019-07-09 00:00:00
abstract::A fiber optic biosensor was used for the fluorimetric detection of T/AT triple-helical DNA formation. The surfaces of two sets of fused silica optical fibers were functionalized with hexaethylene oxide linkers from which decaadenylic acid oligonucleotides were grown in the 3'to 5'and 5'to 3'direction, respectively, us...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.20.4139
更新日期:1997-10-15 00:00:00
abstract::The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonst...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.1.5
更新日期:1993-01-11 00:00:00
abstract::Sequences preceding the minimal promoter play a role in the differential expression of the Xenopus somatic and oocyte-type 5S RNA genes. In this report, the somatic sequences between -32 and +37 are shown to increase transcriptional activity in microinjected embryos, yet have little to no effect in microinjected oocyt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:
更新日期:1989-11-25 00:00:00
abstract::The synthetic DNA fragment (formula, see text) (corresponding to nucleotides 4299-4314 of the phi X DNA sequence) was cloned into either the AmpR gene or the KmR gene of plasmid pACYC 177. The DNA sequence of the KmR gene around the insertion site was determined by nucleotide sequence analysis of the pACYC 177 FnudII ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.14.3335
更新日期:1981-07-24 00:00:00
abstract::Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. For example, the DNA repair complex MutSβ (MSH2-MSH3 heterodimer) is required in mice for on-go...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks810
更新日期:2012-11-01 00:00:00
abstract::Ionizing radiation induces clustered DNA damage, which presents a challenge to the cellular repair machinery. The repair efficiency of a single-strand break (SSB) is approximately 4x less than that for repair of an abasic (AP) site when in a bistranded cluster containing 8-oxoG. To explore whether this difference in r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm947
更新日期:2007-01-01 00:00:00
abstract::EcoCyc is an organism-specific Pathway/Genome Database that describes the metabolic and signal-transduction pathways of Escherichia coli, its enzymes, and-a new addition-its transport proteins. MetaCyc is a new metabolic-pathway database that describes pathways and enzymes of many different organisms, with a microbial...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.56
更新日期:2000-01-01 00:00:00
abstract::Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiRe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx610
更新日期:2017-09-06 00:00:00
abstract::Equilibrative nucleoside transporters (ENTs) are a recently characterized and poorly understood group of membrane proteins that are important in the uptake of endogenous nucleosides required for nucleic acid and nucleoside triphosphate synthesis. Despite their central importance in cellular metabolism and nucleoside a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf564
更新日期:2002-10-15 00:00:00
abstract::31P NMR chemical shifts of the selected mono- and oligonucleotide derivatives, including the compounds with P-N, P-C, P-S bonds and phosphite nucleotide analogues have been presented. The influence of substituents upon 31P chemical shifts has been discussed. The concrete examples of 31P chemical shifts data applicatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.14.5547
更新日期:1984-07-25 00:00:00
abstract::BZ junctions, which connect B-DNA to Z-DNA, are necessary for local transformation of B-DNA to Z-DNA in the genome. However, the limited information on the junction-forming sequences and junction structures has led to a lack of understanding of the structural diversity and sequence preferences of BZ junctions. We dete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky784
更新日期:2018-11-02 00:00:00
abstract::Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNA polymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Polkappa encoded by the DINB1 gene and examined its response to damaged DNA templates...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.21.4138
更新日期:2000-11-01 00:00:00
abstract::We have previously shown that hedamycin, a GC-rich DNA-binding antitumor agent, downregulates survivin transcription (Wu et al. (2005) Molecular mechanism of inhibition of survivin transcription by the GC-rich sequence selective DNA-binding antitumor agent, hedamycin: evidence of survivin downregulation associated wit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm149
更新日期:2007-01-01 00:00:00
abstract::DNA methylation is the best-studied epigenetic modification and describes the conversion of cytosine to 5-methylcytosine. The importance of this phenomenon is that aberrant promoter hypermethylation is a common occurrence in cancer and is frequently associated with gene silencing. Various techniques are currently avai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnj017
更新日期:2006-02-07 00:00:00
abstract::In the battle against viruses, antiviral peptides (AVPs) had demonstrated the immense potential. Presently, more than 15 peptide-based drugs are in various stages of clinical trials. Emerging and re-emerging viruses further emphasize the efforts to accelerate antiviral drug discovery efforts. Despite, huge importance ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks450
更新日期:2012-07-01 00:00:00
abstract::The pol gene of all retroviruses is expressed as a gag-pol fusion protein which is proteolytically processed to produce all viral enzymes. In the human immunodeficiency virus (HIV), the gag and pol genes overlap by 241 nucleotides with pol in the -1 phase with respect to gag. The gag-pol fusion is produced via a -1 ri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.15.3945
更新日期:1992-08-11 00:00:00
abstract::We have isolated the 5'-end of the gene for the rat and human link protein by screening genomic libraries with oligonucleotides corresponding to the 5'-cDNA sequence. Several overlapping clones were isolated for the human link protein gene, while only one clone was obtained for the rat. All the clones contained a sing...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.8.1933
更新日期:1991-04-25 00:00:00
abstract::Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.17.3357
更新日期:1996-09-01 00:00:00
abstract::Pentatricopeptide repeat (PPR) proteins are helical-repeat proteins that offer a promising scaffold for the engineering of proteins to bind specified RNAs. PPR tracts bind RNA in a modular 1-repeat, 1-nucleotide fashion. An amino acid code specifying the bound nucleotide has been elucidated. However, this code does no...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1288
更新日期:2018-03-16 00:00:00
abstract::Genomic DNA of African trypanosomes contains a hypermodified thymidine residue termed base J (beta-d-glucosyl-HOMedU). This modified base is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. The base is synthesized in a two-step pathway. Initially,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn1067
更新日期:2009-04-01 00:00:00
abstract::The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution fol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1349
更新日期:2015-01-01 00:00:00
abstract::The X-ray crystal structure of the complex between the synthetic antitumour and antiviral DNA binding ligand SN 7167 and the DNA oligonucleotide d(CGCGAATTCGCG)2 has been determined to an R factor of 18.3% at 2.6 A resolution. The ligand is located within the minor groove and covers almost 6 bp with the 1-methylpyridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.20.4072
更新日期:1997-10-15 00:00:00
abstract::Structures of r(CGCGCG)2 and 2'-O-Me(CGCGCG)2 have been determined by NMR spectroscopy under low salt conditions. All protons and phosphorus nuclei resonances have been assigned. Signals of H5'/5" have been assigned stereospecifically. All 3JH,H and 3JP,H coupling constants have been measured. The structures were dete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.22.4589
更新日期:1997-11-15 00:00:00
abstract::We found previously that the Chlamydomonas HSP70A promoter counteracts transcriptional silencing of downstream promoters in a transgene setting. To elucidate the underlying mechanisms, we analyzed chromatin state and transgene expression in transformants containing HSP70A-RBCS2-ble (AR-ble) constructs harboring deleti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt224
更新日期:2013-05-01 00:00:00