Minor groove binding of a bis-quaternary ammonium compound: the crystal structure of SN 7167 bound to d(CGCGAATTCGCG)2.

abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...

journal_title：Nucleic acids research

authors： Cupane A,Vitrano E,San Biago PL,Madonia F,Palma MU

更新日期：1980-09-25 00:00:00

abstract：:Two forms of post-transcriptional control direct differential expression of the Saccharomyces cerevisiae genes encoding the AP1-like transcription factors Yap1p and Yap2p. The mRNAs of these genes contain respectively one (YAP1 uORF) and two (YAP2 uORF1 and uORF2) upstream open reading frames. uORF-mediated modulation...

journal_title：Nucleic acids research

authors： Vilela C,Linz B,Rodrigues-Pousada C,McCarthy JE

更新日期：1998-03-01 00:00:00

abstract：:Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

journal_title：Nucleic acids research

authors： Schäfer M,Kunz W

更新日期：1985-02-25 00:00:00

abstract：:The 34-nucleotide trans-activator (TA) located within the RNA-2 of Red clover necrotic mosaic virus folds into a simple hairpin. The eight-nucleotide TA loop base pairs with eight complementary nucleotides in the TA binding sequence (TABS) of the capsid protein subgenomic promoter on RNA-1 and trans-activates subgenom...

journal_title：Nucleic acids research

authors： Guenther RH,Sit TL,Gracz HS,Dolan MA,Townsend HL,Liu G,Newman WH,Agris PF,Lommel SA

更新日期：2004-05-20 00:00:00

abstract：:RNA polymerase II (pol II) utilizes a complex interaction network to select and incorporate correct nucleoside triphosphate (NTP) substrates with high efficiency and fidelity. Our previous 'synthetic nucleic acid substitution' strategy has been successfully applied in dissecting the function of nucleic acid moieties i...

journal_title：Nucleic acids research

authors： Hwang CS,Xu L,Wang W,Ulrich S,Zhang L,Chong J,Shin JH,Huang X,Kool ET,McKenna CE,Wang D

更新日期：2016-05-05 00:00:00

abstract：:The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD act...

journal_title：Nucleic acids research

authors： Smith CA,Shingler V,Thomas CM

更新日期：1984-04-25 00:00:00

abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...

journal_title：Nucleic acids research

authors： Pasternak A,Hernandez FJ,Rasmussen LM,Vester B,Wengel J

更新日期：2011-02-01 00:00:00

abstract：:Procollagen alpha 2 (I) mRNA can be induced congruent to 6-fold in primary avian tendon (PAT) cells on addition of ascorbate to the culture medium. Previously, we have shown that the induction is linear after a 12 h lag and requires a total of 60-72 h to achieve maximum levels. We have now investigated in more detail ...

journal_title：Nucleic acids research

authors： Lyons BL,Schwarz RI

更新日期：1984-03-12 00:00:00

abstract：:Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyt...

journal_title：Nucleic acids research

authors： Wreschner DH,James TC,Silverman RH,Kerr IM

更新日期：1981-04-10 00:00:00

abstract：:The Zebrafish Information Network (ZFIN) (https://zfin.org/) is the database for the model organism, zebrafish (Danio rerio). ZFIN expertly curates, organizes, and provides a wide array of zebrafish genetic and genomic data, including genes, alleles, transgenic lines, gene expression, gene function, mutant phenotypes,...

journal_title：Nucleic acids research

authors： Howe DG,Ramachandran S,Bradford YM,Fashena D,Toro S,Eagle A,Frazer K,Kalita P,Mani P,Martin R,Moxon ST,Paddock H,Pich C,Ruzicka L,Schaper K,Shao X,Singer A,Van Slyke CE,Westerfield M

更新日期：2021-01-08 00:00:00

abstract：:The expression of the nicotinic acetylcholine receptor alpha 2 subunit gene is highly restricted to the Spiriform lateralis nucleus of the Chick diencephalon. As a first step toward understanding the molecular mechanism underlying this regulation, we have investigated the structural and regulatory properties of the 5'...

journal_title：Nucleic acids research

authors： Bessis A,Savatier N,Devillers-Thiéry A,Bejanin S,Changeux JP

更新日期：1993-05-11 00:00:00

abstract：:Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2. The S20,w t...

journal_title：Nucleic acids research

authors： Woodworth-Gutai M,Lebowitz J,Kato AC,Denhardt DT

更新日期：1977-01-01 00:00:00

abstract：:Allele-specific protospacer adjacent motif (asPAM)-positioning SNPs and CRISPRs are valuable resources for gene therapy of dominant disorders. However, one technical hurdle is to identify the haplotype comprising the disease-causing allele and the distal asPAM SNPs. Here, we describe a novel CRISPR-based method (CRISP...

journal_title：Nucleic acids research

authors： Yu J,Xiang X,Huang J,Liang X,Pan X,Dong Z,Petersen TS,Qu K,Yang L,Zhao X,Li S,Zheng T,Xu Z,Liu C,Han P,Xu F,Yang H,Liu X,Zhang X,Bolund L,Luo Y,Lin L

更新日期：2020-03-18 00:00:00

abstract：:Aberrant CpG methylation changes occurring during tumour progression include the loss (hypomethylation) and gain (hypermethylation) of methyl groups. Techniques currently available for examining such changes either require selection of a region, then examination of methylation changes, or utilise methylation-sensitive...

journal_title：Nucleic acids research

authors： Brock GJ,Huang TH,Chen CM,Johnson KJ

更新日期：2001-12-15 00:00:00

abstract：:An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides which have been connected by a flexible polymeric linker chain. As a prototype, binding of this class of oligonucleotide to duplex DNA has been studied using a segment of the HSV-1 D-glycoprotein promoter, which possesses ...

journal_title：Nucleic acids research

authors： Kessler DJ,Pettitt BM,Cheng YK,Smith SR,Jayaraman K,Vu HM,Hogan ME

更新日期：1993-10-11 00:00:00

abstract：:The ProtoNet site provides an automatic hierarchical clustering of the SWISS-PROT protein database. The clustering is based on an all-against-all BLAST similarity search. The similarities' E-score is used to perform a continuous bottom-up clustering process by applying alternative rules for merging clusters. The outco...

journal_title：Nucleic acids research

authors： Sasson O,Vaaknin A,Fleischer H,Portugaly E,Bilu Y,Linial N,Linial M

更新日期：2003-01-01 00:00:00

abstract：:Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresi...

journal_title：Nucleic acids research

authors： Jennerwein MM,Eastman A

更新日期：1991-11-25 00:00:00

abstract：:IntAct is an open-source, open data molecular interaction database populated by data either curated from the literature or from direct data depositions. Two levels of curation are now available within the database, with both IMEx-level annotation and less detailed MIMIx-compatible entries currently supported. As from ...

journal_title：Nucleic acids research

authors： Kerrien S,Aranda B,Breuza L,Bridge A,Broackes-Carter F,Chen C,Duesbury M,Dumousseau M,Feuermann M,Hinz U,Jandrasits C,Jimenez RC,Khadake J,Mahadevan U,Masson P,Pedruzzi I,Pfeiffenberger E,Porras P,Raghunath A,Roeche

更新日期：2012-01-01 00:00:00

abstract：:The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component ...

journal_title：Nucleic acids research

authors： Cribbs DL,Gillam IC,Tener GM

更新日期：1982-10-25 00:00:00

abstract：:A new thermodynamic database for normal and modified nucleic acids has been developed. This Thermodynamic Database for Nucleic Acids (NTDB) includes sequence, structure and thermodynamic information as well as experimental methods and conditions. In this release, there are 1851 sequences containing both normal and mod...

journal_title：Nucleic acids research

authors： Chiu WL,Sze CN,Ip LN,Chan SK,Au-Yeung SC

更新日期：2001-01-01 00:00:00

abstract：:RegulonDB, first published 20 years ago, is a comprehensive electronic resource about regulation of transcription initiation of Escherichia coli K-12 with decades of knowledge from classic molecular biology experiments, and recently also from high-throughput genomic methodologies. We curated the literature to keep Reg...

journal_title：Nucleic acids research

authors： Santos-Zavaleta A,Salgado H,Gama-Castro S,Sánchez-Pérez M,Gómez-Romero L,Ledezma-Tejeida D,García-Sotelo JS,Alquicira-Hernández K,Muñiz-Rascado LJ,Peña-Loredo P,Ishida-Gutiérrez C,Velázquez-Ramírez DA,Del Moral-Chávez V,Bonavid

更新日期：2019-01-08 00:00:00

abstract：:We have isolated genomic clones from Xenopus borealis representing 3 different types of histone gene cluster. We show that the major type (H1, H2B, H2A, H4, H3), present at about 60-70 copies per haploid genome (1), is tandemly reiterated with a repeat length of 15 kb. In situ hybridization to mitotic chromosomes show...

journal_title：Nucleic acids research

authors： Turner PC,Bagenal EB,Vlad MT,Woodland HR

更新日期：1988-04-25 00:00:00

abstract：:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...

journal_title：Nucleic acids research

authors： de Felippes FF,Ott F,Weigel D

更新日期：2011-04-01 00:00:00

abstract：:Nucleotide sequences of large T1 ribonuclease fragments of 18S ribosomal RNA of Novikoff rat ascites hepatoma cells and chicken lymphoblastoid cells were determined and compared. Among the 19 large T1 ribonuclease fragments examined of rat 18S ribosomal RNA, 12 fragments were found to be the same in chicken 18S riboso...

journal_title：Nucleic acids research

authors： Fuke M,Busch H

更新日期：1979-11-10 00:00:00

abstract：:Infection of cultured cells with herpes simplex virus (HSV) results in the transcriptional induction of a small number of cellular genes. Although the majority of such genes are dependent upon viral protein synthesis for their induction, a small minority are not. These genes are induced by events occurring prior to th...

journal_title：Nucleic acids research

authors： Kemp LM,Preston CM,Preston VG,Latchman DS

更新日期：1986-12-09 00:00:00

abstract：:IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to t...

journal_title：Nucleic acids research

authors： Shi L,Qiu D,Zhao G,Corthesy B,Lees-Miller S,Reeves WH,Kao PN

更新日期：2007-01-01 00:00:00

abstract：:We have detected a set of transcripts in Drosophila melanogaster cells which are homologous to repeating elements within the 'non-transcribed' spacer region of rDNA. The RNA molecules range from 240 to 1680 nucleotides, differing in length by an integral value of 240 nucleotides. We have sequenced several AluI fragmen...

journal_title：Nucleic acids research

authors： Miller JR,Hayward DC,Glover DM

更新日期：1983-01-11 00:00:00

abstract：:Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation of double-strand breaks (DSB) and, thus, to deleterious events. The first repair event occurring at a multiply damaged site (MDS) is of major importance and will largely contribute to the hazardousness of MD...

journal_title：Nucleic acids research

authors： Eot-Houllier G,Gonera M,Gasparutto D,Giustranti C,Sage E

更新日期：2007-01-01 00:00:00

abstract：:Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To investigate the true extent of alternative splicing we have devel...

journal_title：Nucleic acids research

authors： Venables JP,Burn J

更新日期：2006-01-01 00:00:00

abstract：:Elongation factor P (EF-P) is a conserved ribosome-binding protein that structurally mimics tRNA to enable the synthesis of peptides containing motifs that otherwise would induce translational stalling, including polyproline. In many bacteria, EF-P function requires post-translational modification with (R)-β-lysine by...

journal_title：Nucleic acids research

authors： Katz A,Solden L,Zou SB,Navarre WW,Ibba M

更新日期：2014-03-01 00:00:00