The structure of tRNA 5 Lys from Drosophila melanogaster.

abstract：:Phosphorylation of the easily accessible 3',5'-diesters 1a-d with diphenyl phosphorochloridate, followed by selective 5'-deacylation, affords the phosphotriester derivatives 2a-d in good yields. Alkaline treatment of 2a-d results in the formation of the 2',3'-cyclic phosphates (3a-d). The usefulness of the phosphotrie...

journal_title：Nucleic acids research

authors： van Boom JH,Burgers PM,Haasnoot CA

更新日期：1976-10-01 00:00:00

abstract：:The database of Clusters of Orthologous Groups of proteins (COGs), which represents an attempt on a phylogenetic classification of the proteins encoded in complete genomes, currently consists of 2791 COGs including 45 350 proteins from 30 genomes of bacteria, archaea and the yeast Saccharomyces cerevisiae (http://www....

journal_title：Nucleic acids research

authors： Tatusov RL,Natale DA,Garkavtsev IV,Tatusova TA,Shankavaram UT,Rao BS,Kiryutin B,Galperin MY,Fedorova ND,Koonin EV

更新日期：2001-01-01 00:00:00

abstract：:Transcription factor (TF) and microRNA (miRNA) are two crucial trans-regulatory factors that coordinately control gene expression. Understanding the impacts of these two factors on the rate of protein sequence evolution is of great importance in evolutionary biology. While many biological factors associated with evolu...

journal_title：Nucleic acids research

authors： Chen YC,Cheng JH,Tsai ZT,Tsai HK,Chuang TJ

更新日期：2013-07-01 00:00:00

abstract：:Reliable prediction of orthology is central to comparative genomics. Approaches based on phylogenetic analyses closely resemble the original definition of orthology and paralogy and are known to be highly accurate. However, the large computational cost associated to these analyses is a limiting factor that often preve...

journal_title：Nucleic acids research

authors： Pryszcz LP,Huerta-Cepas J,Gabaldón T

更新日期：2011-03-01 00:00:00

abstract：:Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system. The analysis of labeled reaction products on specific gel systems suggests that the...

journal_title：Nucleic acids research

authors： Backendorf C,Olsthoorn R,van de Putte P

更新日期：1989-12-25 00:00:00

abstract：:Our web server, PIZSA (http://cospi.iiserpune.ac.in/pizsa), assesses the likelihood of protein-protein interactions by assigning a Z Score computed from interface residue contacts. Our score takes into account the optimal number of atoms that mediate the interaction between pairs of residues and whether these contacts...

journal_title：Nucleic acids research

authors： Roy AA,Dhawanjewar AS,Sharma P,Singh G,Madhusudhan MS

更新日期：2019-07-02 00:00:00

abstract：:Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant ba...

journal_title：Nucleic acids research

authors： Li N,Chen Y,Guo Q,Zhang Y,Yuan Y,Ma C,Deng H,Lei J,Gao N

更新日期：2013-08-01 00:00:00

abstract：:Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced sile...

journal_title：Nucleic acids research

authors： Zhang J,Liu H,Yao Q,Yu X,Chen Y,Cui R,Wu B,Zheng L,Zuo J,Huang Z,Ma J,Gan J

更新日期：2016-11-02 00:00:00

abstract：:OGG1 initiated base excision repair (BER) is the major pathway for repair of oxidative DNA base damage 8-oxoguanine (8-oxoG). Here, we report that RECQL4 DNA helicase, deficient in the cancer-prone and premature aging Rothmund-Thomson syndrome, physically and functionally interacts with OGG1. RECQL4 promotes catalytic...

journal_title：Nucleic acids research

authors： Duan S,Han X,Akbari M,Croteau DL,Rasmussen LJ,Bohr VA

更新日期：2020-07-09 00:00:00

abstract：:The secondary structure of an adenovirus associated low molecular weight RNA (VAI-RNA) has been studied by partial digestion with T1-RNase and S1-endonuclease followed by T1-fingerprint analysis. The empirical secondary structure has been compared with two computer generated models based on minimal free energy of the ...

journal_title：Nucleic acids research

authors： Monstein HJ,Philipson L

更新日期：1981-09-11 00:00:00

abstract：:Prokaryotes use primed CRISPR adaptation to update their memory bank of spacers against invading genetic elements that have escaped CRISPR interference through mutations in their protospacer target site. We previously observed a trend that nucleotide-dependent mismatches between crRNA and the protospacer strongly infl...

journal_title：Nucleic acids research

authors： Künne T,Zhu Y,da Silva F,Konstantinides N,McKenzie RE,Jackson RN,Brouns SJ

更新日期：2018-11-02 00:00:00

abstract：:In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...

journal_title：Nucleic acids research

authors： Okuda K,Shikanai T

更新日期：2012-06-01 00:00:00

abstract：:DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restric...

journal_title：Nucleic acids research

authors： Simpson VJ,Johnson TE,Hammen RF

更新日期：1986-08-26 00:00:00

abstract：:Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent a small fraction of genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach to isolate and sequence gene clusters in metagenomic samp...

journal_title：Nucleic acids research

authors： Xu P,Modavi C,Demaree B,Twigg F,Liang B,Sun C,Zhang W,Abate AR

更新日期：2020-05-07 00:00:00

abstract：:Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be extremely low in these samples and ultra-sensitive methods are required for their detection. Here, we report an extremely simple and fast...

journal_title：Nucleic acids research

authors： Ståhlberg A,Krzyzanowski PM,Jackson JB,Egyud M,Stein L,Godfrey TE

更新日期：2016-06-20 00:00:00

abstract：:Although a vast amount of life sciences data is generated in the form of images, most scientists still store images on extremely diverse and often incompatible storage media, without any type of metadata structure, and thus with no standard facility with which to conduct searches or analyses. Here we present a solutio...

journal_title：Nucleic acids research

authors： Gonzalez-Couto E,Hayes B,Danckaert A

更新日期：2001-01-01 00:00:00

abstract：:DAXX displays complex biological functions. Remarkably, DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Structurally, DAXX is modular with an N-terminal helical bundle, a docking site for many DAXX interactors (e.g. p53 and ...

journal_title：Nucleic acids research

authors： Mahmud I,Liao D

更新日期：2019-09-05 00:00:00

abstract：:Computer programs, implemented on an Univac II00/80 computer system, for the identification and characterization of protein coding genes and for the analysis of nucleic acid sequences, are described. ...

journal_title：Nucleic acids research

authors： Pierno G,Barni N,Candurro M,Cipollaro M,Franzè A,Juliano L,Macchiato MF,Mastrocinque G,Moscatelli C,Scarlato V

更新日期：1984-01-11 00:00:00

abstract：:Mutations in mammalian and Drosophila Hel308 and PolQ paralogues cause genome instability but their helicase functions are mysterious. By in vivo and in vitro analysis, we show that Hel308 from archaea (Hel308a) may act at stalled replication forks. Introducing hel308a into Escherichia coli dnaE strains that condition...

journal_title：Nucleic acids research

authors： Guy CP,Bolt EL

更新日期：2005-06-30 00:00:00

abstract：:LocDB is a manually curated database with experimental annotations for the subcellular localizations of proteins in Homo sapiens (HS, human) and Arabidopsis thaliana (AT, thale cress). Currently, it contains entries for 19,604 UniProt proteins (HS: 13,342; AT: 6262). Each database entry contains the experimentally der...

journal_title：Nucleic acids research

authors： Rastogi S,Rost B

更新日期：2011-01-01 00:00:00

abstract：:The 279-bp major breakpoint region (mbr) within the 3'-untranslated region (3'-UTR) of the BCL2 gene is a binding site of special AT-rich sequence binding protein 1 (SATB1) that is well known to participate in the long-range regulation of gene transcription. Our previous studies have revealed that the mbr could regula...

journal_title：Nucleic acids research

authors： Gong F,Sun L,Wang Z,Shi J,Li W,Wang S,Han X,Sun Y

更新日期：2011-06-01 00:00:00

abstract：:Planarian flatworms are in the midst of a renaissance as a model system for regeneration and stem cells. Besides two well-studied model species, hundreds of species exist worldwide that present a fascinating diversity of regenerative abilities, tissue turnover rates, reproductive strategies and other life history trai...

journal_title：Nucleic acids research

authors： Brandl H,Moon H,Vila-Farré M,Liu SY,Henry I,Rink JC

更新日期：2016-01-04 00:00:00

abstract：:The Yeast Protein Localization database (YPL.db) contains information about the localization patterns of yeast proteins resulting from microscopic analyses. The data and parameters of the experiments to obtain the localization information, together with images from confocal or video microscopy, are stored in a relatio...

journal_title：Nucleic acids research

authors： Habeler G,Natter K,Thallinger GG,Crawford ME,Kohlwein SD,Trajanoski Z

更新日期：2002-01-01 00:00:00

abstract：:The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...

journal_title：Nucleic acids research

authors： York D,Reznikoff WS

更新日期：1996-10-01 00:00:00

abstract：:Bacteria belonging to the class Mollicutes were among the first ones to be selected for complete genome sequencing because of the minimal size of their genomes and their pathogenicity for humans and a broad range of animals and plants. At this time six genome sequences have been publicly released (Mycoplasma genitaliu...

journal_title：Nucleic acids research

authors： Barré A,de Daruvar A,Blanchard A

更新日期：2004-01-01 00:00:00

abstract：:Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of ...

journal_title：Nucleic acids research

authors： Dufour D,de la Peña M,Gago S,Flores R,Gallego J

更新日期：2009-02-01 00:00:00

abstract：:The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important DNA element in diverse systems from specific phages to antibody maturation and Drosophila development. Further relevant investigations would largely benefit from a novel reliable and fast metho...

journal_title：Nucleic acids research

authors： Róna G,Scheer I,Nagy K,Pálinkás HL,Tihanyi G,Borsos M,Békési A,Vértessy BG

更新日期：2016-02-18 00:00:00

abstract：:The interactions of self-complementary oligonucleotides with a group of metal-mediated DNA-binding drugs, including chromomycin A(3), mithramycin and the novel compound UK-1, were examined via electrospray ionization quadrupole ion trap mass spectrometry. Both chromomycin and mithramycin were shown to bind preferentia...

journal_title：Nucleic acids research

authors： Reyzer ML,Brodbelt JS,Kerwin SM,Kumar D

更新日期：2001-11-01 00:00:00

abstract：:Stacking self-association equilibria in aqueous solutions of m3uridine, m34,2',3',5'uridine, 2'-deoxyuridine, m13,4,4cytosine, m14,4,4,5cytosine, s2cytidine and s4thymidine were studied at various temperatures by vapour-pressure osmometry. Equilibrium constants Kst's were computed on the assumption of the isodesmic mo...

journal_title：Nucleic acids research

authors： Plesiewicz E,Stepień E,Bolewska K,Wierzchowski KL

更新日期：1976-05-01 00:00:00

abstract：:A mass culture of human diploid fibroblasts, and eight clones isolated from that mass culture, were examined for methylation patterns in several regions of DNA. Plasmid-inserted cDNA sequences were used as probes for alpha-hCG, beta-globin, A gamma- and G gamma-globin, and beta- and gamma-actin gene regions. Each prob...

journal_title：Nucleic acids research

authors： Shmookler Reis RJ,Goldstein S

更新日期：1982-07-24 00:00:00