当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Ultraviolet light irradiation of PM2 superhelical DNA.

Ultraviolet light irradiation of PM2 superhelical DNA.

Abstract：

:Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2. The S20,w then increases when irradiation is increased to 56,000 ergs/mm2. Thymine dimers are introduced proportional to dose throughtout the range of exposure to u.v. light. Sedimentation velocity-dye titrations reveal anomolous behavior, i.e. apparent increases in superhelix density (sigma). However, the dye-buoyant density procedure showed no change in sigma under the same conditions. The most satisfactory model for the data is preferential photochemical modification of premelted (possibly hairpin) sites as a greater rate than the introduction of photoproducts into duplex sites. The origin of the anomoly in the sedimentation velocity dye titrations is still unclear.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Woodworth-Gutai M,Lebowitz J,Kato AC,Denhardt DT

doi

10.1093/nar/4.5.1243

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

1243-56

issue

5

eissn

0305-1048

issn

1362-4962

journal_volume

4

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Branched co-polymers of histidine and lysine are efficient carriers of plasmids.

    abstract：:We previously determined that a linear co-polymer of histidine and lysine (HK) in combination with liposomes enhanced the transfection efficiency of cationic liposomes. In the current study, we designed a series of HK polymers with increased branching and/or histidine/lysine ratio to determine if either variable affec...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/29.6.1334

    authors： Chen QR,Zhang L,Stass SA,Mixson AJ

    更新日期：2001-03-15 00:00:00

  • The complete nucleotide sequence of the I-E alpha d immune response gene.

    abstract：:We have isolated and sequenced the complete murine I-E alpha immune response gene of the H-2db haplotype. The I-E alpha d gene consists of 5300 basepairs and is organized into five or possibly six exons that correspond to different domains of the alpha chain. The amino acid sequence deduced from the I-E alpha gene sho...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.15.5055

    authors： Hyldig-Nielsen JJ,Schenning L,Hammerling U,Widmark E,Heldin E,Lind P,Servenius B,Lund T,Flavell R,Lee JS,Trowsdale J,Schreier PH,Zablitzky F,Larhammar D,Peterson PA,Rask L

    更新日期：1983-08-11 00:00:00

  • An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.

    abstract：:Genome organization into transcriptionally active domains denotes one of the first levels of gene expression regulation. Although the chromatin domain concept is generally accepted, only little is known on how domain organization impacts the regulation of differential gene expression. Insulators might hold answers to ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq740

    authors： Furlan-Magaril M,Rebollar E,Guerrero G,Fernández A,Moltó E,González-Buendía E,Cantero M,Montoliu L,Recillas-Targa F

    更新日期：2011-01-01 00:00:00

  • Droplet Barcode Sequencing for targeted linked-read haplotyping of single DNA molecules.

    abstract：:Data produced with short-read sequencing technologies result in ambiguous haplotyping and a limited capacity to investigate the full repertoire of biologically relevant forms of genetic variation. The notion of haplotype-resolved sequencing data has recently gained traction to reduce this unwanted ambiguity and enable...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx436

    authors： Redin D,Borgström E,He M,Aghelpasand H,Käller M,Ahmadian A

    更新日期：2017-07-27 00:00:00

  • Synthesis and restriction enzyme analysis of oligodeoxyribonucleotides containing the anti-cancer drug 2',2'-difluoro-2'-deoxycytidine.

    abstract：:The anti-cancer drug 2',2'-difluoro-2'-deoxycytidine (dFdC) is internally incorporated into DNA in vitro. To determine the effects of this incorporation on DNA structure and function, the beta-cyanoethyl phosphoramidite of dFdC was synthesized and oligodeoxyribonucleotides containing dFdC were made using automated sol...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.7.1763

    authors： Richardson FC,Richardson KK,Kroin JS,Hertel LW

    更新日期：1992-04-11 00:00:00

  • An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.

    abstract：:This paper describes a computer program designed to look for similarities between pairs of nucleic or amino acid sequences. The program looks both for segments of perfect identity or for regions where, using a scoring matrix, a minimum value is exceeded. The results of comparisons are presented as a matrix which is di...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/10.9.2951

    authors： Staden R

    更新日期：1982-05-11 00:00:00

  • Cpf1 protein induced bending of yeast centromere DNA element I.

    abstract：:The centromere complex is a multicomponent structure essential for faithful chromosome transmission. Here we show that the S. cerevisiae centromere protein Cpf1 bends centromere DNA element I (CDEI) with the bend angle ranging from 66 degrees to 71 degrees. CDEI DNA sequences that carry point mutations which lead to r...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.20.4726

    authors： Niedenthal RK,Sen-Gupta M,Wilmen A,Hegemann JH

    更新日期：1993-10-11 00:00:00

  • Protein synthesis in rabbit reticulocytes: characteristics of CO-eIF-2 protein complex.

    abstract：:A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNAf binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/10.20.6501

    authors： Bagchi MK,Banerjee AC,Roy R,Chakrabarty I,Gupta NK

    更新日期：1982-10-25 00:00:00

  • Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.

    abstract：:Although the promoters of both the Bax and p21 genes are activated by p53, they differ in the effect on this activation of the POU family transcription factor Brn-3a. Thus, Brn-3a inhibits activation of the Bax promoter by p53 but enhances the ability of p53 to activate the p21 promoter. We demonstrate that repression...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkf610

    authors： Perez-Sanchez C,Budhram-Mahadeo VS,Latchman DS

    更新日期：2002-11-15 00:00:00

  • NetVenn: an integrated network analysis web platform for gene lists.

    abstract：:Many lists containing biological identifiers, such as gene lists, have been generated in various genomics projects. Identifying the overlap among gene lists can enable us to understand the similarities and differences between the data sets. Here, we present an interactome network-based web application platform named N...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku331

    authors： Wang Y,Thilmony R,Gu YQ

    更新日期：2014-07-01 00:00:00

  • SIC: A tool to detect short inverted segments in a biological sequence.

    abstract：:The web software SIC provides a tool to search for short inverted segments (length 3-5000 bp) in a DNA sequence. The sequence is assumed to follow a Markov model. A statistic which is sensitive to inversion is presented. Searching inverted segments is done by a scanning approach after the user specifies the size of th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg596

    authors： Robelin D,Richard H,Prum B

    更新日期：2003-07-01 00:00:00

  • TurboFold II: RNA structural alignment and secondary structure prediction informed by multiple homologs.

    abstract：:This paper presents TurboFold II, an extension of the TurboFold algorithm for predicting secondary structures for multiple RNA homologs. TurboFold II augments the structure prediction capabilities of TurboFold by additionally providing multiple sequence alignments. Probabilities for alignment of nucleotide positions b...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx815

    authors： Tan Z,Fu Y,Sharma G,Mathews DH

    更新日期：2017-11-16 00:00:00

  • Thermodynamic comparison of PNA/DNA and DNA/DNA hybridization reactions at ambient temperature.

    abstract：:The thermodynamics of 13 hybridization reactions between 10 base DNA sequences of design 5'-ATGCXYATGC-3' with X, Y = A, C, G, T and their complementary PNA and DNA sequences were determined from isothermal titration calorimetry (ITC) measurements at ambient temperature. For the PNA/DNA hybridization reactions, the bi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.24.4792

    authors： Schwarz FP,Robinson S,Butler JM

    更新日期：1999-12-15 00:00:00

  • Rice Proteome Database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003.

    abstract：:The Rice Proteome Database is the first detailed database to describe the proteome of rice. The current release contains 21 reference maps based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of proteins from rice tissues and subcellular compartments. These reference maps comprise 11 941 identified pr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkh020

    authors： Komatsu S,Kojima K,Suzuki K,Ozaki K,Higo K

    更新日期：2004-01-01 00:00:00

  • Rational primer design greatly improves differential display-PCR (DD-PCR).

    abstract：:Since its conception in 1992, differential display PCR (DD-PCR) has attracted widespread interest. Theoretically an attractive cloning approach, it combines the comparative analysis of several samples with the sensitivity of PCR. Although a large number of studies embracing this technology have been initiated, few nov...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/25.11.2239

    authors： Graf D,Fisher AG,Merkenschlager M

    更新日期：1997-06-01 00:00:00

  • The DNA binding affinity of HhaI methylase is increased by a single amino acid substitution in the catalytic center.

    abstract：:The HhaI methyltransferase recognizes the sequence GCGC and transfers a methyl group to C5 of the first cytosine residue. All m5C-methyltransferases contain a highly conserved sequence motif called the P-C motif. The cysteine residue of this motif is involved in catalysis by forming a covalent bond with the 6-position...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.10.2459

    authors： Mi S,Roberts RJ

    更新日期：1993-05-25 00:00:00

  • Glycyl-tRNA synthetase specifically binds to the poliovirus IRES to activate translation initiation.

    abstract：:Adaptation to the host cell environment to efficiently take-over the host cell's machinery is crucial in particular for small RNA viruses like picornaviruses that come with only small RNA genomes and replicate exclusively in the cytosol. Their Internal Ribosome Entry Site (IRES) elements are specific RNA structures th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks182

    authors： Andreev DE,Hirnet J,Terenin IM,Dmitriev SE,Niepmann M,Shatsky IN

    更新日期：2012-07-01 00:00:00

  • Versatile vectors to study recoding: conservation of rules between yeast and mammalian cells.

    abstract：:In many viruses and transposons, expression of some genes requires alternative reading of the genetic code, also called recoding. Such events depend on specific mRNA sequences and can lead to read through of an in-frame stop codon or to +1 or -1 frameshifting. Here, we addressed the issue of conservation of recoding r...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.9.1557

    authors： Stahl G,Bidou L,Rousset JP,Cassan M

    更新日期：1995-05-11 00:00:00

  • High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.

    abstract：:We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.10.e45

    authors： Tahiri-Alaoui A,Frigotto L,Manville N,Ibrahim J,Romby P,James W

    更新日期：2002-05-15 00:00:00

  • An RNA-based transcription activator derived from an inhibitory aptamer.

    abstract：:According to the recruitment model of transcriptional activation, an activator helps initiate transcription by bringing the RNA polymerase to a specific location on the DNA through interaction with components of the transcriptional machinery. However, it is difficult to isolate and define the activities of specific ac...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp1227

    authors： Wang S,Shepard JR,Shi H

    更新日期：2010-04-01 00:00:00

  • Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.

    abstract：:Although the involvement of Ser/Arg-rich (SR) proteins in RNA metabolism is well documented, their role in vertebrate development remains elusive. We, therefore, elected to take advantage of the zebrafish model organism to study the SR genes' functions using the splicing morpholino (sMO) microinjection and the program...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx638

    authors： Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

    更新日期：2017-09-19 00:00:00

  • Importance of thiols in the repair mechanisms of DNA containing AP (apurinic or apyrimidinic) sites.

    abstract：:Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.20.9489

    authors： Bailly V,Verly WG

    更新日期：1988-10-25 00:00:00

  • Survey of chimeric IStron elements in bacterial genomes: multiple molecular symbioses between group I intron ribozymes and DNA transposons.

    abstract：:IStrons are chimeric genetic elements composed of a group I intron associated with an insertion sequence (IS). The group I intron is a catalytic RNA providing the IStron with self-splicing ability, which renders IStron insertions harmless to the host genome. The IS element is a DNA transposon conferring mobility, and ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku939

    authors： Tourasse NJ,Stabell FB,Kolstø AB

    更新日期：2014-11-10 00:00:00

  • Requirements for selective recruitment of Ets proteins and activation of mb-1/Ig-alpha gene transcription by Pax-5 (BSAP).

    abstract：:Pax-5, a member of the paired domain family of transcription factors, is a key regulator of B lymphocyte-specific transcription and differentiation. A major target of Pax-5-mediated activation is the mb-1 gene, which encodes the essential transmembrane signaling protein Ig-alpha. Pax-5 recruits three members of the Et...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg785

    authors： Maier H,Ostraat R,Parenti S,Fitzsimmons D,Abraham LJ,Garvie CW,Hagman J

    更新日期：2003-10-01 00:00:00

  • Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan.

    abstract：:The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw1026

    authors： Meisenberg C,Ashour ME,El-Shafie L,Liao C,Hodgson A,Pilborough A,Khurram SA,Downs JA,Ward SE,El-Khamisy SF

    更新日期：2017-02-17 00:00:00

  • Drosophila melanogaster Dis3 N-terminal domains are required for ribonuclease activities, nuclear localization and exosome interactions.

    abstract：:Eukaryotic cells use numerous pathways to regulate RNA production, localization and stability. Several of these pathways are controlled by ribonucleases. The essential ribonuclease, Dis3, plays important roles in distinct RNA metabolic pathways. Despite much progress in understanding general characteristics of the Dis...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq295

    authors： Mamolen M,Smith A,Andrulis ED

    更新日期：2010-09-01 00:00:00

  • Gene amplification in methotrexate-resistant mouse cells. IV. Different DNA sequences are amplified in different resistant lines.

    abstract：:DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/10.21.6597

    authors： Caizzi R,Bostock CJ

    更新日期：1982-11-11 00:00:00

  • UTRdb and UTRsite (RELEASE 2010): a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs.

    abstract：:The 5' and 3' untranslated regions of eukaryotic mRNAs (UTRs) play crucial roles in the post-transcriptional regulation of gene expression through the modulation of nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and message stability. UTRdb is a curated database of 5' and 3' untran...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp902

    authors： Grillo G,Turi A,Licciulli F,Mignone F,Liuni S,Banfi S,Gennarino VA,Horner DS,Pavesi G,Picardi E,Pesole G

    更新日期：2010-01-01 00:00:00

  • Sequencing of an upstream region of the human HLA-DRA gene containing X' and Y' boxes.

    abstract：:In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.10.1671

    authors： Feriotto G,Mischiati C,Bianchi N,Rutigliano C,Giacomini P,Gambari R

    更新日期：1995-05-25 00:00:00

  • Structural activation of the transcriptional repressor EthR from Mycobacterium tuberculosis by single amino acid change mimicking natural and synthetic ligands.

    abstract：:Ethionamide is an antituberculous drug for the treatment of multidrug-resistant Mycobacterium tuberculosis. This antibiotic requires activation by the monooxygenase EthA to exert its activity. Production of EthA is controlled by the transcriptional repressor EthR, a member of the TetR family. The sensitivity of M. tub...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr1113

    authors： Carette X,Blondiaux N,Willery E,Hoos S,Lecat-Guillet N,Lens Z,Wohlkönig A,Wintjens R,Soror SH,Frénois F,Dirié B,Villeret V,England P,Lippens G,Deprez B,Locht C,Willand N,Baulard AR

    更新日期：2012-04-01 00:00:00