Abstract:
:DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, and that the library is essentially complete for sequences contained in Bam H1 restriction fragments between 9 and 19 kb. The inserts of some selected lambda-recombinants were subcloned in pBR328 or pAT153 to separate sequences of differing repetition frequency. Clones representative of different classes of sequences were used as probes to Southern transfers of Bam H1 digested total nuclear DNAs of various MTX-resistant cell lines. The results clearly show that the amplified unit of each cell line has a unique structure, and that different amplified units differ widely in their sequence composition.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Caizzi R,Bostock CJdoi
10.1093/nar/10.21.6597subject
Has Abstractpub_date
1982-11-11 00:00:00pages
6597-618issue
21eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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