Abstract：
:The multifunctional RNA-dependent RNA polymerase L protein of vesicular stomatitis virus catalyzes unconventional pre-mRNA capping via the covalent enzyme-pRNA intermediate formation, which requires the histidine-arginine (HR) motif in the polyribonucleotidyltransferase domain. Here, the effects of cap-defective mutations in the HR motif on transcription were analyzed using an in vitro reconstituted transcription system. The wild-type L protein synthesized the leader RNA from the 3'-end of the genome followed by 5'-capped and 3'-polyadenylated mRNAs from internal genes by a stop-start transcription mechanism. Cap-defective mutants efficiently produced the leader RNA, but displayed aberrant stop-start transcription using cryptic termination and initiation signals within the first gene, resulting in sequential generation of ∼40-nucleotide transcripts with 5'-ATP from a correct mRNA-start site followed by a 28-nucleotide transcript and long 3'-polyadenylated transcript initiated with non-canonical GTP from atypical start sites. Frequent transcription termination and re-initiation within the first gene significantly attenuated the production of downstream mRNAs. Consistent with the inability of these mutants in in vitro mRNA synthesis and capping, these mutations were lethal to virus replication in cultured cells. These findings indicate that viral mRNA capping is required for accurate stop-start transcription as well as mRNA stability and translation and, therefore, for virus replication in host cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ogino Tdoi
10.1093/nar/gku901subject
Has Abstractpub_date
2014-10-29 00:00:00pages
12112-25issue
19eissn
0305-1048issn
1362-4962pii
gku901journal_volume
42pub_type
杂志文章abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp853
更新日期：2010-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.8.999
更新日期：1974-08-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2014-11-10 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.8.1979
更新日期：1992-04-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1982-09-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1997-06-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：1992-03-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1975-05-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2018-10-12 00:00:00
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journal_title：Nucleic acids research
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更新日期：2007-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1995-11-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1992-04-11 00:00:00
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pub_type： 杂志文章
doi：10.1093/nar/gkx1227
更新日期：2018-03-16 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2014-12-16 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1992-06-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1982-02-11 00:00:00
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更新日期：2002-11-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：1980-02-11 00:00:00
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更新日期：2011-02-01 00:00:00
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更新日期：1976-05-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：1992-12-11 00:00:00
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更新日期：2012-07-01 00:00:00
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更新日期：2019-07-26 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
