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Importance of thiols in the repair mechanisms of DNA containing AP (apurinic or apyrimidinic) sites.

Abstract：

:Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-O-P bond 3' to an AP site: hydrolysis or beta-elimination. Addition of thiols to the double-bond of the 3'-terminal sugar resulting from beta-elimination prevents a subsequent delta-elimination. The interpretation of the action of enzymes that start by nicking 3' to AP sites must take into account the presence or absence of thiols in the reaction medium. In living cells, thiols might influence the pathways followed by the repair processes of AP site-containing DNA.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Bailly V,Verly WG

doi

10.1093/nar/16.20.9489

subject

Has Abstract

pub_date

1988-10-25 00:00:00

pages

9489-96

issue

20

eissn

0305-1048

issn

1362-4962

journal_volume

16

pub_type

杂志文章

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