Transcription of the 'non-transcribed' spacer of Drosophila melanogaster rDNA.

abstract：:To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Al...

journal_title：Nucleic acids research

authors： Barrett MT,Reid BJ,Joslyn G

更新日期：1995-09-11 00:00:00

abstract：:Allele-specific protospacer adjacent motif (asPAM)-positioning SNPs and CRISPRs are valuable resources for gene therapy of dominant disorders. However, one technical hurdle is to identify the haplotype comprising the disease-causing allele and the distal asPAM SNPs. Here, we describe a novel CRISPR-based method (CRISP...

journal_title：Nucleic acids research

authors： Yu J,Xiang X,Huang J,Liang X,Pan X,Dong Z,Petersen TS,Qu K,Yang L,Zhao X,Li S,Zheng T,Xu Z,Liu C,Han P,Xu F,Yang H,Liu X,Zhang X,Bolund L,Luo Y,Lin L

更新日期：2020-03-18 00:00:00

abstract：:PIWI-interacting RNAs (piRNAs) engage PIWI proteins to silence transposons and promote germ cell development in animals. In diverse species, piRNA biogenesis occurs near the mitochondrial surface, and involves mitochondrial membrane-anchored factors. In mice, two cytoplasmic PIWI proteins, MIWI and MILI, receive proce...

journal_title：Nucleic acids research

authors： Ding D,Liu J,Dong K,Melnick AF,Latham KE,Chen C

更新日期：2019-03-18 00:00:00

abstract：:Transcript of the rbpA1 gene in Anabaena variabilis accumulates significantly at low growth temperatures below 28 degreesC. This accumulation was maximal at 16 degreesC. Accumulation of the rbpA1 transcript was completely abolished by rifampicin, but not by chloramphenicol. Photosynthesis was not required for this col...

journal_title：Nucleic acids research

authors： Sato N,Nakamura A

更新日期：1998-05-01 00:00:00

abstract：:In an RNase III-deficient strain of E. coli 23S pre-rRNA accumulates unprocessed in 50S ribosomes and in polysomes. These ribosomes provide a substrate for the analysis of rRNA maturation in vitro. S1 nuclease protection analysis of the products obtained in in vitro processing reactions demonstrates that 23S rRNA proc...

journal_title：Nucleic acids research

authors： Sirdeshmukh R,Schlessinger D

更新日期：1985-07-25 00:00:00

abstract：:Guanine rich nucleic acid sequences can form G-quadruplex (G4) structures that interfere with DNA replication, repair and RNA transcription. The human FANCJ helicase contributes to maintaining genomic integrity by promoting DNA replication through G4-forming DNA regions. Here, we combined single-molecule and ensemble ...

journal_title：Nucleic acids research

authors： Wu CG,Spies M

更新日期：2016-10-14 00:00:00

abstract：:Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket. In addition, it often crucially depends on the stability of peripheral structural elements. The ligand-bound complex of the guanine-sensing riboswitch fr...

journal_title：Nucleic acids research

authors： Buck J,Wacker A,Warkentin E,Wöhnert J,Wirmer-Bartoschek J,Schwalbe H

更新日期：2011-12-01 00:00:00

abstract：:We have developed an effective, easy-to-use two-step system for the site-directed insertion of large genetic constructs into arbitrary positions in the Escherichia coli chromosome. The system uses lambda-Red mediated recombineering accompanied by the introduction of double-strand DNA breaks in the chromosome and a don...

journal_title：Nucleic acids research

authors： Kuhlman TE,Cox EC

更新日期：2010-04-01 00:00:00

abstract：:The enzyme endonuclease V nicks uracil-containing DNA at the second or third phosphodiester bond 3' to uracil sites. I applied the enzyme to random fragmentation of DNA to revise the complex DNA shuffling protocol. The merit of using endonuclease V is that cleavage occurs at random sites and the length of the fragment...

journal_title：Nucleic acids research

authors： Miyazaki K

更新日期：2002-12-15 00:00:00

abstract：:In order to facilitate the direct computation of the sizes of DNA fragments separated by gel electrophoresis, we have written and evaluated programmes for the Hewlett-Packard 41C programmable calculator. The sizes estimated for DNA fragments of known length using some of these programmes were found to be more accurate...

journal_title：Nucleic acids research

authors： Gough EJ,Gough NM

更新日期：1984-01-11 00:00:00

abstract：:In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase ...

journal_title：Nucleic acids research

authors： Fodor I,Matvienko NI,Zlotnikov KM,Tanyashin VI,Solonin AS,Bayev AA

更新日期：1975-05-01 00:00:00

abstract：:Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties simi...

journal_title：Nucleic acids research

authors： Kielanowska M,Shugar D

更新日期：1976-03-01 00:00:00

abstract：:Differential melting profiles and denaturation maps are calculated for fdDNA whose sequence of nucleotides has been determined recently. The melting profiles for the total DNA and a number of its restriction fragments are compared with experimental data taken from literature. The comparison enables one to correlate a ...

journal_title：Nucleic acids research

authors： Vologodskii AV,Frank-Kamenetskii MD

更新日期：1978-07-01 00:00:00

abstract：:In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show t...

journal_title：Nucleic acids research

authors： Chevrier-Miller M,Jacques N,Raibaud O,Dreyfus M

更新日期：1990-10-11 00:00:00

abstract：:The hypoxia-inducible factor-1 (HIF-1) was first described as a DNA binding activity that specifically recognizes an 8 bp motif known to be essential for hypoxia-inducible erythropoietin gene transcription. Subsequently HIF-1 activity has also been found in cell lines which do not express erythropoietin, suggesting th...

journal_title：Nucleic acids research

authors： Kvietikova I,Wenger RH,Marti HH,Gassmann M

更新日期：1995-11-25 00:00:00

abstract：:To bridge the gap between the rising information needs of biological and medical researchers and the rapidly growing number of online bioinformatics resources, we have created the Online Bioinformatics Resources Collection (OBRC) at the Health Sciences Library System (HSLS) at the University of Pittsburgh. The OBRC, c...

journal_title：Nucleic acids research

authors： Chen YB,Chattopadhyay A,Bergen P,Gadd C,Tannery N

更新日期：2007-01-01 00:00:00

abstract：:A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species. ...

journal_title：Nucleic acids research

authors： Kafatos FC,Jones CW,Efstratiadis A

更新日期：1979-11-24 00:00:00

abstract：:Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of MAR-containing vec...

journal_title：Nucleic acids research

authors： Grandjean M,Girod PA,Calabrese D,Kostyrko K,Wicht M,Yerly F,Mazza C,Beckmann JS,Martinet D,Mermod N

更新日期：2011-08-01 00:00:00

abstract：:A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...

journal_title：Nucleic acids research

authors： Wiebers JL

更新日期：1976-11-01 00:00:00

abstract：:T4 polynucleotide kinase (Pnk) is a bifunctional 5'-kinase/3'-phosphatase that aids in the repair of broken termini in RNA by converting 3'-PO4/5'-OH ends into 3'-OH/5'-PO4 ends, which are then sealed by RNA ligase. Here we have employed site-directed mutagenesis (introducing 31 mutations at 16 positions) to locate ca...

journal_title：Nucleic acids research

authors： Wang LK,Shuman S

更新日期：2002-02-15 00:00:00

abstract：:Infrared spectroscopy was used to identify hydration-sensitive structural differences between single- stranded phosphorothioate (PS) and phosphodiester (PO) oligodeoxyribonucleotides. Spectra were recorded in the mid-infrared region, 500-1800 cm-1, at relative humidities between 0 and 98%; the PS and PO spectra are su...

journal_title：Nucleic acids research

authors： White AP,Reeves KK,Snyder E,Farrell J,Powell JW,Mohan V,Griffey RH

更新日期：1996-08-15 00:00:00

abstract：:A highly sensitive method to detect traces of aldehyde-containing apurinic/apyrimidinic (AP) sites in nucleic acids has been developed. Based on this method, a novel approach to detect DNA base mismatches recognized by the mismatch repair glycosylase MutY is demonstrated. Open chain aldehydes generated in nucleic acid...

journal_title：Nucleic acids research

authors： Maulik G,Botchway S,Chakrabarti S,Tetradis S,Price B,Makrigiorgos GM

更新日期：1999-03-01 00:00:00

abstract：:The primary structures of two overlapping novel alpha-tubulin cDNA clones isolated from a Macaca fascicularis testis cDNA library and the corresponding human gene are presented. Although the general structure of the human gene conforms to that of previously described mammalian alpha-tubulin genes, there is a surprisin...

journal_title：Nucleic acids research

authors： Dobner PR,Kislauskis E,Wentworth BM,Villa-Komaroff L

更新日期：1987-01-12 00:00:00

abstract：:A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has ...

journal_title：Nucleic acids research

authors： Gingeras TR,MIlazzo JP,Roberts RJ

更新日期：1978-11-01 00:00:00

abstract：:Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...

journal_title：Nucleic acids research

authors： Ferrando A,Koncz-Kálmán Z,Farràs R,Tiburcio A,Schell J,Koncz C

更新日期：2001-09-01 00:00:00

abstract：:Recent studies have shown that homologous recombination (HR) requires chromatin repression as well as relaxation at DNA double strand breaks (DSBs). HP1 and SUV39H1/2 are repressive factors essential for HR. Here, we identify SETDB1 as an additional compacting factor promoting HR. Depletion of HP1, SUV39, SETDB1 or BR...

journal_title：Nucleic acids research

authors： Alagoz M,Katsuki Y,Ogiwara H,Ogi T,Shibata A,Kakarougkas A,Jeggo P

更新日期：2015-09-18 00:00:00

abstract：:The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...

journal_title：Nucleic acids research

authors： Jones WR,Stone MP

更新日期：1998-02-15 00:00:00

abstract：:FASTQ has emerged as a common file format for sharing sequencing read data combining both the sequence and an associated per base quality score, despite lacking any formal definition to date, and existing in at least three incompatible variants. This article defines the FASTQ format, covering the original Sanger stand...

journal_title：Nucleic acids research

authors： Cock PJ,Fields CJ,Goto N,Heuer ML,Rice PM

更新日期：2010-04-01 00:00:00

abstract：:The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone alpha-factor. We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene was found 5' of an open reading frame ...

journal_title：Nucleic acids research

authors： Burkholder AC,Hartwell LH

更新日期：1985-12-09 00:00:00

abstract：:We have developed a new bioinformatics approach called ECMFinder (Evolutionary Conserved Motif Finder). This program searches for a given DNA motif within the entire genome of one species and uses the gene association information of a potential transcription factor-binding site (TFBS) to screen the homologous regions ...

journal_title：Nucleic acids research

authors： Kang K,Chung JH,Kim J

更新日期：2009-04-01 00:00:00