A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination.

abstract：:Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of t...

journal_title：Nucleic acids research

authors： Huang CH,Baserga R

更新日期：1976-08-01 00:00:00

abstract：:Ribosome recycling factor (RRF), elongation factor G (EF-G) and GTP split 70S ribosomes into subunits. Here, we demonstrated that the splitting was transient and the exhaustion of GTP resulted in re-association of the split subunits into 70S ribosomes unless IF3 (initiation factor 3) was present. However, the splittin...

journal_title：Nucleic acids research

authors： Hirokawa G,Iwakura N,Kaji A,Kaji H

更新日期：2008-12-01 00:00:00

abstract：:The effects of the treatment of cultured human and simian cells with Cadmium (Cd), a toxic and carcinogenic metal, were first assayed on macromolecular synthesis. It was observed that DNA synthesis was inhibited by Cd concentrations considerably lower than those inhibiting protein and RNA synthesis. Because of the nec...

journal_title：Nucleic acids research

authors： Nocentini S

更新日期：1987-05-26 00:00:00

abstract：:Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their...

journal_title：Nucleic acids research

authors： Potter BV,Eckstein F,Uznański B

更新日期：1983-10-25 00:00:00

abstract：:Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisatio...

journal_title：Nucleic acids research

authors： Guerasimova A,Ivanov I,Lehrach H

更新日期：1999-01-15 00:00:00

abstract：:The centromere is a highly specialized chromosomal element that is essential for chromosome segregation during mitosis. Centromere integrity must therefore be properly preserved and is strictly dependent upon the establishment and maintenance of surrounding chromatin structure. Here we identify WDHD1, a WD40-domain an...

journal_title：Nucleic acids research

authors： Hsieh CL,Lin CL,Liu H,Chang YJ,Shih CJ,Zhong CZ,Lee SC,Tan BC

更新日期：2011-05-01 00:00:00

abstract：:MCScan is an algorithm able to scan multiple genomes or subgenomes in order to identify putative homologous chromosomal regions, and align these regions using genes as anchors. The MCScanX toolkit implements an adjusted MCScan algorithm for detection of synteny and collinearity that extends the original software by in...

journal_title：Nucleic acids research

authors： Wang Y,Tang H,Debarry JD,Tan X,Li J,Wang X,Lee TH,Jin H,Marler B,Guo H,Kissinger JC,Paterson AH

更新日期：2012-04-01 00:00:00

abstract：:As proteomics initiatives mature, the need will arise for the multiple visualization of proteins and supramolecular complexes within their true context, in situ. Single-stranded DNA and RNA aptamers can be used for low resolution imaging of cellular receptors and cytoplasmic proteins by light microscopy (LM). These te...

journal_title：Nucleic acids research

authors： Dellaire G,Nisman R,Eskiw CH,Bazett-Jones DP

更新日期：2004-11-23 00:00:00

abstract：:High mobility group protein B1 (HMGB1) binds to the internucleosomal linker DNA in chromatin and abuts the nucleosome. Bending and untwisting of the linker DNA results in transmission of strain to the nucleosome core, disrupting histone/DNA contacts. An interaction between H3 and HMGB1 has been reported. Here we confi...

journal_title：Nucleic acids research

authors： Watson M,Stott K,Fischl H,Cato L,Thomas JO

更新日期：2014-01-01 00:00:00

abstract：:PrimPol is a primase-polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been established. Here, we report that the proposed zinc finger domain ...

journal_title：Nucleic acids research

authors： Keen BA,Jozwiakowski SK,Bailey LJ,Bianchi J,Doherty AJ

更新日期：2014-05-01 00:00:00

abstract：:IGF2 mRNA-binding protein 1 (IMP1) is a key regulator of messenger RNA (mRNA) metabolism and transport in organismal development and, in cancer, its mis-regulation is an important component of tumour metastasis. IMP1 function relies on the recognition of a diverse set of mRNA targets that is mediated by the combinator...

journal_title：Nucleic acids research

authors： Dagil R,Ball NJ,Ogrodowicz RW,Hobor F,Purkiss AG,Kelly G,Martin SR,Taylor IA,Ramos A

更新日期：2019-05-07 00:00:00

abstract：:A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Strongylocentrotus purpuratus has been characterized, and shown to contain the sequence features which indicate that ...

journal_title：Nucleic acids research

authors： Nisson PE,Hickey RJ,Boshar MF,Crain WR Jr

更新日期：1988-02-25 00:00:00

abstract：:The cnjC gene from the protozoan Tetrahymena thermophila was completely sequenced. The deduced gene product was found to have significant sequence similarity to the yeast and prokaryotic RNA polymerase subunits involved with subunit assembly. Since cnjC is active only during the sexual stage (conjugation) of Tetrahyme...

journal_title：Nucleic acids research

authors： Martindale DW

更新日期：1990-05-25 00:00:00

abstract：:The increasing availability of multi-omic platforms poses new challenges to data analysis. Joint visualization of multi-omics data is instrumental in better understanding interconnections across molecular layers and in fully utilizing the multi-omic resources available to make biological discoveries. We present here P...

journal_title：Nucleic acids research

authors： Hernández-de-Diego R,Tarazona S,Martínez-Mira C,Balzano-Nogueira L,Furió-Tarí P,Pappas GJ Jr,Conesa A

更新日期：2018-07-02 00:00:00

abstract：:OGG1 initiated base excision repair (BER) is the major pathway for repair of oxidative DNA base damage 8-oxoguanine (8-oxoG). Here, we report that RECQL4 DNA helicase, deficient in the cancer-prone and premature aging Rothmund-Thomson syndrome, physically and functionally interacts with OGG1. RECQL4 promotes catalytic...

journal_title：Nucleic acids research

authors： Duan S,Han X,Akbari M,Croteau DL,Rasmussen LJ,Bohr VA

更新日期：2020-07-09 00:00:00

abstract：:Transcriptional interference between adjacent genes has been demonstrated in a variety of biological systems. To study this process in RNA polymerase II (pol II) transcribed genes we have analysed the effect of transcription on tandem HIV-1 promoters integrated into the genome of HeLa cells. We show that transcription...

journal_title：Nucleic acids research

authors： Greger IH,Demarchi F,Giacca M,Proudfoot NJ

更新日期：1998-03-01 00:00:00

abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...

journal_title：Nucleic acids research

authors： Castroviejo M,Tarragó-Litvak L,Litvak S

更新日期：1975-11-01 00:00:00

abstract：:The advent of RNA-sequencing and microarray technologies has led to rapid growth of transcriptome data generated for a wide range of organisms, under various cellular, organ and individual conditions. Since the number of possible combinations of intercellular and extracellular conditions is almost unlimited, catalogin...

journal_title：Nucleic acids research

authors： Obayashi T,Kagaya Y,Aoki Y,Tadaka S,Kinoshita K

更新日期：2019-01-08 00:00:00

abstract：:Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...

journal_title：Nucleic acids research

authors： Pech A,Achenbach J,Jahnz M,Schülzchen S,Jarosch F,Bordusa F,Klussmann S

更新日期：2017-04-20 00:00:00

abstract：:The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity...

journal_title：Nucleic acids research

authors： Felber BK,Gerber-Huber S,Meier C,May FE,Westley B,Weber R,Ryffel GU

更新日期：1981-06-11 00:00:00

abstract：:p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...

journal_title：Nucleic acids research

authors： Al-Mohanna MA,Al-Khalaf HH,Al-Yousef N,Aboussekhra A

更新日期：2007-01-01 00:00:00

abstract：:Pathogenic bacteria encounter host-imposed manganese (Mn) limitation during infection. Herein we report that in the human pathogen Streptococcus pyogenes, the adaptive response to Mn limitation is controlled by a DtxR family metalloregulator, MtsR. Genes upregulated by MtsR during Mn limitation include Mn (mtsABC) and...

journal_title：Nucleic acids research

authors： Do H,Makthal N,Chandrangsu P,Olsen RJ,Helmann JD,Musser JM,Kumaraswami M

更新日期：2019-08-22 00:00:00

abstract：:A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activ...

journal_title：Nucleic acids research

authors： Mellado RP,Salas M

更新日期：1983-11-11 00:00:00

abstract：:Folding of group II introns is characterized by a first slow compaction of domain 1 (D1) followed by the rapid docking of other domains to this scaffold. D1 compaction initiates in a small subregion encompassing the κ and ζ elements. These two tertiary elements are also the major interaction sites with domain 5 to for...

journal_title：Nucleic acids research

authors： Donghi D,Pechlaner M,Finazzo C,Knobloch B,Sigel RK

更新日期：2013-02-01 00:00:00

abstract：:Recent research hints at an underappreciated complexity in pre-miRNA processing and regulation. Global profiling of pre-miRNA and its potential to increase understanding of the pre-miRNA landscape is impeded by overlap with highly expressed classes of other non coding (nc) RNA. Here, we present a data set excluding th...

journal_title：Nucleic acids research

authors： Burroughs AM,Kawano M,Ando Y,Daub CO,Hayashizaki Y

更新日期：2012-02-01 00:00:00

abstract：:Members of the basic helix-loop-helix (bHLH) family of transcription factors regulate a wide array of developmental processes in many cell types, including cell fate specification, differentiation and morphogenesis. Our studies describe the cloning of a gene from the nematode Caenorhabditis elegans that is closely rel...

journal_title：Nucleic acids research

authors： Nguyen L,Round J,O'Connell R,Geurts P,Funes-Duran M,Wong J,Jongeward G,Vierra CA

更新日期：2001-11-01 00:00:00

abstract：:In Saccharomyces cerevisiae, RNA polymerase II assembly is probably initiated by the formation of the RPB3-RPB11 heterodimer. RPB3 is encoded by a single copy gene in the yeast, mouse and human genomes. The RPB11 gene is also unique in yeast and mouse, but in humans a gene family has been identified that potentially e...

journal_title：Nucleic acids research

authors： Benga WJ,Grandemange S,Shpakovski GV,Shematorova EK,Kedinger C,Vigneron M

更新日期：2005-06-24 00:00:00

abstract：:Transcription and mRNA export are linked processes. However, the molecular mechanisms of this coordination are not clear. Sus1 (hENY2) participates in this coordination as part of two protein complexes: SAGA, a transcriptional co-activator; TREX-2, which functions in mRNA biogenesis and export. Here, we investigate th...

journal_title：Nucleic acids research

authors： García-Oliver E,Pascual-García P,García-Molinero V,Lenstra TL,Holstege FC,Rodríguez-Navarro S

更新日期：2013-06-01 00:00:00

abstract：:In silico prediction of transcription factor binding sites (TFBSs) is central to the task of gene regulatory network elucidation. Genomic DNA sequence information provides a basis for these predictions, due to the sequence specificity of TF-binding events. However, DNA sequence alone is an impoverished source of infor...

journal_title：Nucleic acids research

authors： Whitington T,Perkins AC,Bailey TL

更新日期：2009-01-01 00:00:00

abstract：:Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic Acids Res., 20, 1691-1693]. Here we describe a multiplex form of SDA that allows two target sequences and an internal amplification control to be co-amplified by...

journal_title：Nucleic acids research

authors： Walker GT,Nadeau JG,Spears PA,Schram JL,Nycz CM,Shank DD

更新日期：1994-07-11 00:00:00