Circular dichroism studies of ethidium bromide binding to the isolated nucleolus.

abstract：:Many, if not all, mRNAs in T.brucei start with the same sequence of 35 nucleotides, separately encoded in clustered so-called mini-exon repeats. From these mini-exon repeats a 141-nt precursor RNA with the 35-nt sequence at its 5' end is transcribed. Indirect evidence suggests that this RNA is linked in a second step ...

journal_title：Nucleic acids research

authors： Kooter JM,Borst P

更新日期：1984-12-21 00:00:00

abstract：:Mixed-lineage leukemia (MLL) maintains the expression of cellular memory genes during development, while leukemic MLL fusion proteins aberrantly maintain expression of hematopoietic stem cell program genes such as HOXA9 to cause leukemia. However, the molecular mechanism of gene activation is unclear. Here we show tha...

journal_title：Nucleic acids research

authors： Okuda H,Kawaguchi M,Kanai A,Matsui H,Kawamura T,Inaba T,Kitabayashi I,Yokoyama A

更新日期：2014-04-01 00:00:00

abstract：:The Fos and Jun family of transcription factors contain an invariant sequence motif lysine-cysteine-arginine (KCR) in the highly conserved DNA-binding region. Reduction of the cysteine residue is necessary to facilitate DNA-binding. Here, we examined the potential dual roles of the flanking lysine and arginine residue...

journal_title：Nucleic acids research

authors： Ng L,Forrest D,Curran T

更新日期：1993-12-25 00:00:00

abstract：:The thymine analog 5-chlorouridine, first reported in the 1950s as anti-tumor agent, is known as an effective mutagen, clastogen and toxicant as well as an effective inducer of sister-chromatid exchange. Recently, the first microorganism with a chemically different genome was reported; the selected Escherichia coli st...

journal_title：Nucleic acids research

authors： Patra A,Harp J,Pallan PS,Zhao L,Abramov M,Herdewijn P,Egli M

更新日期：2013-02-01 00:00:00

abstract：:The variation of the proton chemical shifts due to the formation intermolecular hydrogen bonds is computed for a number of complexes which can be formed between the bases of the nucleic acids. The shifts expected for the isolated base pairs, in particular for the G-N1 H, T(or U)-N3H protons and the protons of the amin...

journal_title：Nucleic acids research

authors： Giessner-Prettre C,Pullman B,Caillet J

更新日期：1977-01-01 00:00:00

abstract：:We have previously investigated the role of the N-terminal region of ribosomal protein S4 to participate in 30S ribosome assembly and function (1-3). In this report we extend these studies to the two fragments produced by the chemical cleavage of protein S4 at the tryptophan residue 167. We find that the carboxyl term...

journal_title：Nucleic acids research

authors： Changchien LM,Craven GR

更新日期：1985-09-11 00:00:00

abstract：:Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2...

journal_title：Nucleic acids research

authors： Singer B,Pergolizzi RG,Grunberger D

更新日期：1979-04-01 00:00:00

abstract：:Bile acid biosynthesis is subjected to feedback regulation whereby bile acids down-regulate their own synthesis. The major point of this regulation is at the level of cholesterol 7alpha-hydroxylase (7alpha-hydroxylase), which controls bile acid output from the classic pathway. This regulation is at the level of transc...

journal_title：Nucleic acids research

authors： del Castillo-Olivares A,Gil G

更新日期：2000-09-15 00:00:00

abstract：:By screening a rabbit reticulocyte library, an alternative 15-LOX transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was predominantly expressed in non-erythr...

journal_title：Nucleic acids research

authors： Thiele BJ,Berger M,Huth A,Reimann I,Schwarz K,Thiele H

更新日期：1999-04-15 00:00:00

abstract：:The chromatin structure of yeast ribosomal DNA was analyzed in vivo by crosslinking intact cells with psoralen. We found that in exponentially growing cultures the regions coding for the 35S rRNA precursor fall into two distinct classes. One class was highly accessible to psoralen and associated with nascent RNAs, cha...

journal_title：Nucleic acids research

authors： Dammann R,Lucchini R,Koller T,Sogo JM

更新日期：1993-05-25 00:00:00

abstract：:The products of the RAG-1 and RAG-2 genes are essential for the recombination of the DNA encoding the antigen receptors of the developing immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing loca...

journal_title：Nucleic acids research

authors： Sadofsky MJ,Hesse JE,McBlane JF,Gellert M

更新日期：1993-12-11 00:00:00

abstract：:SIRT6 is critical for activating transcription of Nuclear factor (erythroid-derived 2)-like 2 (NRF2) responsive genes during oxidative stress. However, while the mechanism of SIRT6-mediated silencing is well understood, the mechanism of SIRT6-mediated transcriptional activation is unknown. Here, we employed SIRT6 sepa...

journal_title：Nucleic acids research

authors： Rezazadeh S,Yang D,Tombline G,Simon M,Regan SP,Seluanov A,Gorbunova V

更新日期：2019-09-05 00:00:00

abstract：:The histone variant H2A.Z has been implicated in many biological processes, such as gene regulation and genome stability. Here, we present the identification of H2A.Z.2.2 (Z.2.2), a novel alternatively spliced variant of histone H2A.Z and provide a comprehensive characterization of its expression and chromatin incorpo...

journal_title：Nucleic acids research

authors： Bönisch C,Schneider K,Pünzeler S,Wiedemann SM,Bielmeier C,Bocola M,Eberl HC,Kuegel W,Neumann J,Kremmer E,Leonhardt H,Mann M,Michaelis J,Schermelleh L,Hake SB

更新日期：2012-07-01 00:00:00

abstract：:Understanding the molecular mechanisms that influence transposable element target site preferences is a fundamental challenge in functional and evolutionary genomics. Large-scale transposon insertion projects provide excellent material to study target site preferences in the absence of confounding effects of post-inse...

journal_title：Nucleic acids research

authors： Linheiro RS,Bergman CM

更新日期：2008-11-01 00:00:00

abstract：:We have investigated the attachment of the DNA to the nuclear matrix during the division cycle of the plasmodial slime mold Physarum polycephalum. The DNA of plasmodia was pulse labelled at different times during the S phase and the label distribution was studied by graded DNase digestion of the matrix-DNA complexes p...

journal_title：Nucleic acids research

authors： Aelen JM,Opstelten RJ,Wanka F

更新日期：1983-02-25 00:00:00

abstract：:Here we report that the poor binding of methylphosphonate oligodeoxynucleosides (MP-ODNs) to their nucleic acid targets can be improved by additional inversion of the anomeric configuration (from beta to alpha) in the sugar moieties to give a new class of analogs, MP alpha-oligonucleosides. MP alpha-dT12and MP 5' alph...

journal_title：Nucleic acids research

authors： Debart F,Meyer A,Vasseur JJ,Rayner B

更新日期：1998-10-15 00:00:00

abstract：:enod40 is a plant gene that participates in the regulation of symbiotic interaction between leguminous plants and bacteria or fungi. Furthermore, it has been suggested to play a general role in non-symbiotic plant development. Although enod40 seems to have multiple functions, being present in many land plants, the mol...

journal_title：Nucleic acids research

authors： Gultyaev AP,Roussis A

更新日期：2007-01-01 00:00:00

abstract：:Previous studies have established a critical role of both TFIIB and RNA polymerase II (RNAPII) in start site selection in the yeast Saccharomyces cerevisiae. However, it remains unclear how the TFIIB-RNAPII interaction impacts on this process since such an interaction can potentially influence both preinitiation compl...

journal_title：Nucleic acids research

authors： Zhang DY,Carson DJ,Ma J

更新日期：2002-07-15 00:00:00

abstract：:Termination of protein synthesis is promoted in ribosomes by proper stop codon discrimination by class 1 polypeptide release factors (RFs). A large set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational metho...

journal_title：Nucleic acids research

authors： Oparina NJ,Kalinina OV,Gelfand MS,Kisselev LL

更新日期：2005-09-14 00:00:00

abstract：:A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction...

journal_title：Nucleic acids research

authors： Watkins PC,Tanzi RE,Gibbons KT,Tricoli JV,Landes G,Eddy R,Shows TB,Gusella JF

更新日期：1985-09-11 00:00:00

abstract：:Specific targeting of protein–nucleic acid interactions is an area of current interest, for example, in the regulation of gene-expression. Most transcription factor proteins bind in the DNA major groove; however, we are interested in an approach using small molecules to target the minor groove to control expression by...

journal_title：Nucleic acids research

authors： Laughlin-Toth S,Carter EK,Ivanov I,Wilson WD

更新日期：2017-02-17 00:00:00

abstract：:A simple, reproducible affinity chromatography method has been adapted for separation of high molecular weight supercoiled circular molecules from mammalian cells. Electron microscopic analysis of EB viral DNA obtained by this method, from the non-producer Burkitt's lymphoma line Raji, revealed monomer-sized viral mol...

journal_title：Nucleic acids research

authors： Kinchington D,Griffin BE

更新日期：1987-12-23 00:00:00

abstract：:Bacterial genomes encode a plethora of small RNAs (sRNAs), which are heterogeneous in size, structure and function. Most sRNAs act as post-transcriptional regulators by means of specific base pairing interactions with the 5'-untranslated region of mRNA transcripts, thereby modifying the stability of the target transcr...

journal_title：Nucleic acids research

authors： Eggenhofer F,Tafer H,Stadler PF,Hofacker IL

更新日期：2011-07-01 00:00:00

abstract：:The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational effi...

journal_title：Nucleic acids research

authors： Gallie DR,Feder JN,Schimke RT,Walbot V

更新日期：1991-09-25 00:00:00

abstract：:RNase D was recently reported as a new enzymatic activity associated with HIV-1 reverse transcriptase (RT), cleaving RNA at two positions within the double-stranded region of the tRNA primer-viral RNA template complex (Ben-Artzi et al., Proc. Natl. Acad. Sci. USA 89 (1992) 927-931). This would make RNase D a fourth di...

journal_title：Nucleic acids research

authors： Hostomsky Z,Hudson GO,Rahmati S,Hostomska Z

更新日期：1992-11-11 00:00:00

abstract：:The nucleotide sequences of the Streptomyces transposable element IS110 and its insertion site in the DNA of a derivative of the temperate phage luminal diameter C31 were determined. The element is inserted about 460 bp from the right-hand end of luminal diameter C31 DNA, in a region of apparently non-coding DNA. The ...

journal_title：Nucleic acids research

authors： Bruton CJ,Chater KF

更新日期：1987-09-11 00:00:00

abstract：:To confirm the activity of an initial small molecule 'hit compound' from an activity screening, one needs to probe the structure-activity relationships by testing close analogs. The multi-fingerprint browser presented here (http://dcb-reymond23.unibe.ch:8080/MCSS/) enables one to rapidly identify such close analogs am...

journal_title：Nucleic acids research

authors： Awale M,Reymond JL

更新日期：2014-07-01 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli K12 available from the GENBANK and EMBO databases and over a period of several years independently from the literature. We have introduced all available genetic map data and have arranged the sequences accordingly. As far as possible the overlaps are deleted and a tot...

journal_title：Nucleic acids research

authors： Kröger M

更新日期：1989-01-01 00:00:00

abstract：:Recombinant plasmids which contain EcoRI fragments of tobacco chloroplast DNA carrying tRNA genes were constructed. Plasmids pTC211 and pTC293 contain the base sequences for tRNAAsn in their 1.4 and 1.1 Md EcoRI fragments, respectively. These two tRNA sequences are identical and are; 5'-TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGC...

journal_title：Nucleic acids research

authors： Kato A,Shimada H,Kusuda M,Sugiura M

更新日期：1981-11-11 00:00:00

abstract：:Orthology is a widely used concept in comparative and evolutionary genomics. In addition to prokaryotic orthology, delineating eukaryotic orthology has provided insight into the evolution of higher organisms. Indeed, many eukaryotic ortholog databases have been established for this purpose. However, unlike prokaryotes...

journal_title：Nucleic acids research

authors： Ho MR,Jang WJ,Chen CH,Ch'ang LY,Lin WC

更新日期：2008-06-01 00:00:00