Abstract:
:The products of the RAG-1 and RAG-2 genes are essential for the recombination of the DNA encoding the antigen receptors of the developing immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination function. In addition, a series of small deletions within the strongly conserved remaining 60% of the coding region was tested. Nine out of ten of these prove unable to provide RAG-1 activity, but one is quite active. Certain peptide sequences were also specifically targeted for mutagenesis. The RAG-1 protein generated from this expression system is transported to the nucleus and is degraded with a 15 minute half-life. The fate of the proteins made by the deletion mutants were also assessed. Transport of RAG-1 protein to the nucleus was found even with the most extensive deletions studied. The functionality of the deleted proteins is discussed with relation to an alignment of RAG-1 sequences from five animal species.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sadofsky MJ,Hesse JE,McBlane JF,Gellert Mdoi
10.1093/nar/21.24.5644subject
Has Abstractpub_date
1993-12-11 00:00:00pages
5644-50issue
24eissn
0305-1048issn
1362-4962journal_volume
21pub_type
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