Abstract:
:Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By multimerizing the MDB domain of Mbd1, we engineered a poly-MBD protein that displays methyl-CpG-specific binding in vitro with a dissociation constant that is >50-fold higher than that of a monomeric MBD. Poly-MBD proteins also localize to methylated foci in cells and can deliver a functional domain to reporter constructs in vivo. We propose that poly-MBD proteins are sensitive reagents for the detection of DNA methylation levels in isolated native DNA and for cytological detection of chromosomal CpG methylation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Jørgensen HF,Adie K,Chaubert P,Bird APdoi
10.1093/nar/gkl527subject
Has Abstractpub_date
2006-08-07 00:00:00pages
e96issue
13eissn
0305-1048issn
1362-4962pii
34/13/e96journal_volume
34pub_type
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