Abstract:
:Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Olal D,Dick A,Woods VL Jr,Liu T,Li S,Devignot S,Weber F,Saphire EO,Daumke Odoi
10.1093/nar/gku229subject
Has Abstractpub_date
2014-05-01 00:00:00pages
6025-37issue
9eissn
0305-1048issn
1362-4962pii
gku229journal_volume
42pub_type
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