Mediation of the A/B-DNA helix transition by G-tracts in the crystal structure of duplex CATGGGCCCATG.

abstract：:The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR...

journal_title：Nucleic acids research

authors： Bruder JT,Heidecker G,Tan TH,Weske JC,Derse D,Rapp UR

更新日期：1993-11-11 00:00:00

abstract：:Base excision repair (BER), which is initiated by DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. The NTHL1 glycosylase, which excises DNA base damage caused by reactive oxygen species, is thought to be a tumor suppressor. However, in addition to NTHL1 loss-of-function...

journal_title：Nucleic acids research

authors： Limpose KL,Trego KS,Li Z,Leung SW,Sarker AH,Shah JA,Ramalingam SS,Werner EM,Dynan WS,Cooper PK,Corbett AH,Doetsch PW

更新日期：2018-05-18 00:00:00

abstract：:Here, we present an update of Breast Cancer Now Tissue Bank bioinformatics, a rich platform for the sharing, mining, integration and analysis of breast cancer data. Its modalities provide researchers with access to a centralised information gateway from which they can access a network of bioinformatic resources to que...

journal_title：Nucleic acids research

authors： Gadaleta E,Pirrò S,Dayem Ullah AZ,Marzec J,Chelala C

更新日期：2018-01-04 00:00:00

abstract：:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...

journal_title：Nucleic acids research

authors： Pyle LE,Finch LR

更新日期：1988-07-11 00:00:00

abstract：:Multiplexed high-throughput pyrosequencing is currently limited in complexity (number of samples sequenced in parallel), and in capacity (number of sequences obtained per sample). Physical-space segregation of the sequencing platform into a fixed number of channels allows limited multiplexing, but obscures available s...

journal_title：Nucleic acids research

authors： Parameswaran P,Jalili R,Tao L,Shokralla S,Gharizadeh B,Ronaghi M,Fire AZ

更新日期：2007-01-01 00:00:00

abstract：:DNA molecules with covalently sealed (hairpin) ends are probable intermediates in V(D)J recombination. According to current models hairpin ends are opened to produce short single-stranded extensions that are thought to be precursors of a particular type of extra nucleotides, termed P nucleotides, which are frequently ...

journal_title：Nucleic acids research

authors： Kabotyanski EB,Zhu C,Kallick DA,Roth DB

更新日期：1995-10-11 00:00:00

abstract：:The sequence of 110 nucleotides adjacent to the 3'-end of Neurospora crassa 25S-rRNA has been derived by chemical sequencing methods. Sequences present between 40 and 85 nucleotides of the 3'-end were found to complement sequences at the 3'- and 5'-ends of 5.8S-rRNA. Interaction was shown to occur between 5.8S-rRNA an...

journal_title：Nucleic acids research

authors： Kelly JM,Cox RA

更新日期：1981-03-11 00:00:00

abstract：:E. coli C6 rel- met- cys- was cultured in a fully supplemented medium and in media lacking cysteine or methionine. tRNA isolated from the three cultures containted, respectively, a normal complement of modified nucleosides; a deficiency in thiolated nucleosides and a deficiency in methylated nucleosides. Both sulfur-d...

journal_title：Nucleic acids research

authors： Agris PF,Armstrong DJ,Schäfer KP,Söll D

更新日期：1975-05-01 00:00:00

abstract：:Cisplatin analogues were synthesised that consisted of platinum(II) diamine complexes tethered via a polymethylene chain ( n = 3, 5, 8 and 10) to a phenanthridinium cation. Both chloro and iodo leaving groups were examined. DNA adduct formation was quantitatively analysed using a linear amplification system with the p...

journal_title：Nucleic acids research

authors： Whittaker J,McFadyen WD,Wickham G,Wakelin LP,Murray V

更新日期：1998-09-01 00:00:00

abstract：:The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiatio...

journal_title：Nucleic acids research

authors： Pickett GG,Peabody DS

更新日期：1993-09-25 00:00:00

abstract：:Plasmodium falciparum origin recognition complex 1 (ORC1) protein has been implicated in DNA replication and silencing var gene family. However, the mechanism and the domain structure of ORC1 related to the regulation of var gene family are unknown. Here we show that the unique N-terminus of PfORC1 (PfORC1N(1-238)) is...

journal_title：Nucleic acids research

authors： Deshmukh AS,Srivastava S,Herrmann S,Gupta A,Mitra P,Gilberger TW,Dhar SK

更新日期：2012-07-01 00:00:00

abstract：:DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable 'scar' sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinato...

journal_title：Nucleic acids research

authors： Taylor GM,Mordaka PM,Heap JT

更新日期：2019-02-20 00:00:00

abstract：:We present a new coarse grained method for the simulation of duplex DNA. The algorithm uses a generalized multi-harmonic model that can represent any multi-normal distribution of helical parameters, thus avoiding caveats of current mesoscopic models for DNA simulation and representing a breakthrough in the field. The ...

journal_title：Nucleic acids research

authors： Walther J,Dans PD,Balaceanu A,Hospital A,Bayarri G,Orozco M

更新日期：2020-03-18 00:00:00

abstract：:As proteomics initiatives mature, the need will arise for the multiple visualization of proteins and supramolecular complexes within their true context, in situ. Single-stranded DNA and RNA aptamers can be used for low resolution imaging of cellular receptors and cytoplasmic proteins by light microscopy (LM). These te...

journal_title：Nucleic acids research

authors： Dellaire G,Nisman R,Eskiw CH,Bazett-Jones DP

更新日期：2004-11-23 00:00:00

abstract：:Potential promoters in the genome of Escherichia coli were searched by pattern recognition software PlatProm and classified on the basis of positions relative to gene borders. Beside the expected promoters located in front of the coding sequences we found a considerable amount of intragenic promoter-like signals with ...

journal_title：Nucleic acids research

authors： Shavkunov KS,Masulis IS,Tutukina MN,Deev AA,Ozoline ON

更新日期：2009-08-01 00:00:00

abstract：:Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...

journal_title：Nucleic acids research

authors： Stephens DC,Poon GM

更新日期：2016-10-14 00:00:00

abstract：:A normal human population has been screened for the existence of further restriction fragment length polymorphisms (RFLPs) in the clotting factor IX gene in addition to the TaqI polymorphism already characterised (1,2). Two polymorphic loci were found, both within 6 Kb of the TaqI polymorphism within the body of the f...

journal_title：Nucleic acids research

authors： Winship PR,Anson DS,Rizza CR,Brownlee GG

更新日期：1984-12-11 00:00:00

abstract：:Plants have large diverse families of small secreted proteins (SSPs) that play critical roles in the processes of development, differentiation, defense, flowering, stress response, symbiosis, etc. Oryza sativa is one of the major crops worldwide and an excellent model for monocotyledonous plants. However, there had no...

journal_title：Nucleic acids research

authors： Pan B,Sheng J,Sun W,Zhao Y,Hao P,Li X

更新日期：2013-01-01 00:00:00

abstract：:Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal ...

journal_title：Nucleic acids research

authors： Kermekchiev MB,Tzekov A,Barnes WM

更新日期：2003-11-01 00:00:00

abstract：:Competition binding and UV melting studies of a DNA model system consisting of three, four or five mutually complementary oligonucleotides demonstrate that unpaired bases at the branch point stabilize three- and five-way junction loops but destabilize four-way junctions. The inclusion of unpaired nucleotides permits t...

journal_title：Nucleic acids research

authors： Kadrmas JL,Ravin AJ,Leontis NB

更新日期：1995-06-25 00:00:00

abstract：:Gap closure is a challenging phase in microbial random shotgun genome sequencing projects, particularly since genome assemblies are often complicated by the presence of repeat elements, insertion sequences and other similar factors that contribute to sequence misassemblies. While it is well recognized that the conserv...

journal_title：Nucleic acids research

authors： Herron-Olson L,Freeman J,Zhang Q,Retzel EF,Kapur V

更新日期：2003-09-01 00:00:00

abstract：:Recent studies show that the region of DNA brought into close proximity to the fetal gamma-globin genes in deletional forms of hereditary persistence of fetal hemoglobin (HPFH) is selectively hypomethylated (and presumably active) in normal erythroid tissue. This region is normally located approximately 100 kilobases ...

journal_title：Nucleic acids research

authors： Poncz M,Sutton M,Delgrosso K,Schwartz E,Surrey S

更新日期：1987-07-10 00:00:00

abstract：:Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange™ mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom ...

journal_title：Nucleic acids research

authors： Bond SR,Naus CC

更新日期：2012-07-01 00:00:00

abstract：:The 5' and 3' untranslated regions of eukaryotic mRNAs (UTRs) play crucial roles in the post-transcriptional regulation of gene expression through the modulation of nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and message stability. UTRdb is a curated database of 5' and 3' untran...

journal_title：Nucleic acids research

authors： Grillo G,Turi A,Licciulli F,Mignone F,Liuni S,Banfi S,Gennarino VA,Horner DS,Pavesi G,Picardi E,Pesole G

更新日期：2010-01-01 00:00:00

abstract：:Proteins interacting with pre-mRNAs during early stages of spliceosome formation in a HeLa nuclear extract were investigated by photochemical RNA-protein crosslinking. The level of protein crosslinking to a beta-globin pre-mRNA was positively correlated with the presence of an intron. Proteins of 110,000, 59,000 and 3...

journal_title：Nucleic acids research

authors： Mayrand SH,Pederson T

更新日期：1990-06-11 00:00:00

abstract：:Strategies to regulate gene function frequently use small interfering RNAs (siRNAs) that can be made from their shRNA precursors via Dicer. However, when the duplex components of these siRNA effectors are expressed from their respective coding genes, the RNA interference (RNAi) activity is much reduced. Here, we explo...

journal_title：Nucleic acids research

authors： Jin X,Sun T,Zhao C,Zheng Y,Zhang Y,Cai W,He Q,Taira K,Zhang L,Zhou D

更新日期：2012-02-01 00:00:00

abstract：:Protein p56 (56 amino acids) from the Bacillus subtilis phage 29 inactivates the host uracil-DNA glycosylase (UDG), an enzyme involved in the base excision repair pathway. At present, p56 is the only known example of a UDG inhibitor encoded by a non-uracil containing viral DNA. Using analytical ultracentrifugation met...

journal_title：Nucleic acids research

authors： Serrano-Heras G,Ruiz-Masó JA,del Solar G,Espinosa M,Bravo A,Salas M

更新日期：2007-01-01 00:00:00

abstract：:A fundamental problem with applying Consensus, Weight-Matrix or hidden Markov models as search tools for biosequences is that there is no way to know, from the model, if the modeled sequences display any dependencies between positional identities. In some instances, these dependencies are crucial in correctly acceptin...

journal_title：Nucleic acids research

authors： Ray WC

更新日期：2005-07-01 00:00:00

abstract：:Mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (PLRV) P1 (ORF1) protein were applied to the analysis of P1 expression during PLRV replication in planta. Western analyses detected P1 and a protein of approximately 25 kDa (P1-C25) that accumulated to readily detectab...

journal_title：Nucleic acids research

authors： Prüfer D,Kawchuk L,Monecke2 M,Nowok S,Fischer R,Rohde W

更新日期：1999-01-15 00:00:00

abstract：:We have studied the effects of the nucleotide analogue, 2',3'-dideoxythymidine-5'-triphosphate (ddTTP) on replicative DNA synthesis in HeLa cell lysates. As previously demonstrated (1), such lysates carry out extensive DNA synthesis in vitro, at rates and in a fashion similar to in vivo DNA replication. We report here...

journal_title：Nucleic acids research

authors： Waqar MA,Evans MJ,Huberman JA

更新日期：1978-06-01 00:00:00