Genotypic analysis of multiple loci in somatic cells by whole genome amplification.

abstract：:Gap closure is a challenging phase in microbial random shotgun genome sequencing projects, particularly since genome assemblies are often complicated by the presence of repeat elements, insertion sequences and other similar factors that contribute to sequence misassemblies. While it is well recognized that the conserv...

journal_title：Nucleic acids research

authors： Herron-Olson L,Freeman J,Zhang Q,Retzel EF,Kapur V

更新日期：2003-09-01 00:00:00

abstract：:Structural changes of reconstituted SV 40 minichromosomes have been studied in relation to the salt concentration and addition of histone H1 by sedimentation and electron microscopy. Sedimentation data are represented as functions of the NaCl concentration and the Debye-Hückel electrostatic screening radius 1/alpha. T...

journal_title：Nucleic acids research

authors： Böttger M,Scherneck S,von Mickwitz CU,Fenske H,Lindigkeit R

更新日期：1979-08-10 00:00:00

abstract：:DNA-PKcs and Ku are essential components of the complex that catalyzes non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs). Ku, a heterodimeric protein, binds to DNA ends and facilitates recruitment of the catalytic subunit, DNA-PKcs. We have investigated the effect of DNA strand orientation and seque...

journal_title：Nucleic acids research

authors： Pawelczak KS,Andrews BJ,Turchi JJ

更新日期：2005-01-07 00:00:00

abstract：:Hydroxylapatite chromatographies of mitochondrial and total enzymes from a rho+ yeast, or from the related rho degrees mitochondrial DNA-less mutant, show the occurrence in the mitochondrial enzyme of one Phe-, one Met-, one Leu-tRNA synthetase peak which elutes distinctly from the cytoplasmic counterpart and charges ...

journal_title：Nucleic acids research

authors： Schneller JM,Schneller C,Martin R,Stahl AJ

更新日期：1976-05-01 00:00:00

abstract：:When designed to cleave a target RNA in trans, the hammerhead ribozyme contains two antisense flanks which form helix I and helix III by pairing with the complementary target RNA. The sequences forming helix II are contained on the ribozyme strand and represent a major structural component of the hammerhead structure....

journal_title：Nucleic acids research

authors： Homann M,Tabler M,Tzortzakaki S,Sczakiel G

更新日期：1994-09-25 00:00:00

abstract：:We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by the restriction enzym...

journal_title：Nucleic acids research

authors： Germann MW,Kalisch BW,Lundberg P,Vogel HJ,van de Sande JH

更新日期：1990-03-25 00:00:00

abstract：:Identification of common sub-sequences for a group of functionally related DNA sequences can shed light on the role of such elements in cell-specific gene expression. In the megakaryocytic lineage, no one single unique transcription factor was described as linage specific, raising the possibility that a cluster of gen...

journal_title：Nucleic acids research

authors： Hazony Y,Lu J,St Hilaire C,Ravid K

更新日期：2006-01-01 00:00:00

abstract：:Mutations in the gene of human RNase T2 are associated with white matter disease of the human brain. Although brain abnormalities (bilateral temporal lobe cysts and multifocal white matter lesions) and clinical symptoms (psychomotor impairments, spasticity and epilepsy) are well characterized, the pathomechanism of RN...

journal_title：Nucleic acids research

authors： Thorn A,Steinfeld R,Ziegenbein M,Grapp M,Hsiao HH,Urlaub H,Sheldrick GM,Gärtner J,Krätzner R

更新日期：2012-09-01 00:00:00

abstract：:Strategies to regulate gene function frequently use small interfering RNAs (siRNAs) that can be made from their shRNA precursors via Dicer. However, when the duplex components of these siRNA effectors are expressed from their respective coding genes, the RNA interference (RNAi) activity is much reduced. Here, we explo...

journal_title：Nucleic acids research

authors： Jin X,Sun T,Zhao C,Zheng Y,Zhang Y,Cai W,He Q,Taira K,Zhang L,Zhou D

更新日期：2012-02-01 00:00:00

abstract：:The satellite RNA of tobacco ringspot virus (STobRV RNA) replicates and becomes encapsidated in association with tobacco ringspot virus. Previous results show that the infected tissue produces multimeric STobRV RNAs of both polarities. RNA that is complementary to encapsidated STobRV RNA, designated as having the (-) ...

journal_title：Nucleic acids research

authors： Buzayan JM,Hampel A,Bruening G

更新日期：1986-12-22 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen with a relatively large genome, and has been shown to routinely lose genomic fragments during environmental selection. However, the underlying molecular mechanisms that promote chromosomal deletion are still poorly understood. In a recent study, we showed that by del...

journal_title：Nucleic acids research

authors： Shen M,Zhang H,Shen W,Zou Z,Lu S,Li G,He X,Agnello M,Shi W,Hu F,Le S

更新日期：2018-05-18 00:00:00

abstract：:Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from th...

journal_title：Nucleic acids research

authors： Huang YQ,Rehfuss RP,LaPlante SR,Boudreau E,Borer PN,Lane MJ

更新日期：1988-12-09 00:00:00

abstract：:We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1s-1. We find that GT-containing oligonucleotides bind much fa...

journal_title：Nucleic acids research

authors： Paes HM,Fox KR

更新日期：1997-08-15 00:00:00

abstract：:The X-ray crystal structure of the complex between the synthetic antitumour and antiviral DNA binding ligand SN 7167 and the DNA oligonucleotide d(CGCGAATTCGCG)2 has been determined to an R factor of 18.3% at 2.6 A resolution. The ligand is located within the minor groove and covers almost 6 bp with the 1-methylpyridi...

journal_title：Nucleic acids research

authors： Squire CJ,Clark GR,Denny WA

更新日期：1997-10-15 00:00:00

abstract：:A requirement for specific RNA folding is that the free-energy landscape discriminate against non-native folds. While tertiary interactions are critical for stabilizing the native fold, they are relatively non-specific, suggesting additional mechanisms contribute to tertiary folding specificity. In this study, we use ...

journal_title：Nucleic acids research

authors： Mustoe AM,Al-Hashimi HM,Brooks CL 3rd

更新日期：2016-01-08 00:00:00

abstract：:We have recently isolated a functional promoter encoding the human polypeptide-binding protein (BiP) gene from Burkitt's lymphoma cells by polymerase chain reaction (The EMBL Data Library accession number X59969, 1991). This promoter DNA segment (termed BiP670) was fused to the bacterial chloramphenicol acetyltransfer...

journal_title：Nucleic acids research

authors： Chao CC,Lin-Chao S

更新日期：1992-12-25 00:00:00

abstract：:Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...

journal_title：Nucleic acids research

authors： Han YH,Kim DK,Na TY,Ka NL,Choi HS,Lee MO

更新日期：2016-02-18 00:00:00

abstract：:The antitumour antibiotics bleomycin and actinomycin are commonly used therapeutically in combination. One causes metal ion- and oxygen-dependent oxidative damage to DNA, while the other acts at the level of DNA via intercalation of its phenoxazone chromophore and probable inhibition of topoisomerases. Both drugs bind...

journal_title：Nucleic acids research

authors： Bailly C,Kénani A,Waring MJ

更新日期：1997-04-15 00:00:00

abstract：:The ribosomal frameshifting signal of the mouse embryonal carcinoma differentiation regulated (Edr) gene represents the sole documented example of programmed -1 frameshifting in mammalian cellular genes [Shigemoto,K., Brennan,J., Walls,E,. Watson,C.J., Stott,D., Rigby,P.W. and Reith,A.D. (2001), Nucleic Acids Res., 29...

journal_title：Nucleic acids research

authors： Manktelow E,Shigemoto K,Brierley I

更新日期：2005-03-14 00:00:00

abstract：:The highly charged DNA chain may be either in an extended conformation, the coil, or condensed into a highly dense and ordered structure, the toroid. The transition, also called collapse of the chain, can be triggered in different ways, for example by changing the ionic conditions of the solution. We observe individua...

journal_title：Nucleic acids research

authors： Sung B,Leforestier A,Livolant F

更新日期：2016-02-18 00:00:00

abstract：:The DNA replication, plasmid segregation and transactivation functions of Epstein-Barr nuclear antigen 1 (EBNA1) require the binding of EBNA1 to specific DNA recognition sites in the two non-contiguous functional elements of the Epstein-Barr virus latent origin of replication, oriP . EBNA1 molecules bound to these ele...

journal_title：Nucleic acids research

authors： Avolio-Hunter TM,Frappier L

更新日期：1998-10-01 00:00:00

abstract：:The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...

journal_title：Nucleic acids research

authors： Méjean V,Devedjian JC,Rives I,Alloing G,Claverys JP

更新日期：1991-10-25 00:00:00

abstract：:In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...

journal_title：Nucleic acids research

authors： Okuda K,Shikanai T

更新日期：2012-06-01 00:00:00

abstract：:We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...

journal_title：Nucleic acids research

authors： Mergny JL,Collier D,Rougée M,Montenay-Garestier T,Hélène C

更新日期：1991-04-11 00:00:00

abstract：:An essential coordinator of all DNA metabolic processes is Replication Protein A (RPA). RPA orchestrates these processes by binding to single-stranded DNA (ssDNA) and interacting with several other DNA binding proteins. Determining the real-time kinetics of single players such as RPA in the presence of multiple DNA pr...

journal_title：Nucleic acids research

authors： Pokhrel N,Origanti S,Davenport EP,Gandhi D,Kaniecki K,Mehl RA,Greene EC,Dockendorff C,Antony E

更新日期：2017-09-19 00:00:00

abstract：:The accurate structural modeling of B- and T-cell receptors is fundamental to gain a detailed insight in the mechanisms underlying immunity and in developing new drugs and therapies. The LYRA (LYmphocyte Receptor Automated modeling) web server (http://www.cbs.dtu.dk/services/LYRA/) implements a complete and automated ...

journal_title：Nucleic acids research

authors： Klausen MS,Anderson MV,Jespersen MC,Nielsen M,Marcatili P

更新日期：2015-07-01 00:00:00

abstract：:Antisense oligodeoxynucleotides (ODNs) have biological activity in treating various forms of cancer. The antisense effects of two types of 20mer ODNs, phosphorothioate-modified ODNs (S-ODNs) and S-ODNs with 12 2'-O-methyl groups (Me-S-ODNs), targeted to sites 109 and 277 of bcl-2 mRNA, were compared. Both types were a...

journal_title：Nucleic acids research

authors： Yoo BH,Bochkareva E,Bochkarev A,Mou TC,Gray DM

更新日期：2004-04-02 00:00:00

abstract：:The eukaryotic ribosomal protein S26e (rpS26e) lacking eubacterial counterparts is a key component of the ribosomal binding site of mRNA region 5' of the codon positioned at the exit site. Here, we determined the rpS26e oligopeptide neighboring mRNA on the human 80S ribosome using mRNA analogues bearing perfluoropheny...

journal_title：Nucleic acids research

authors： Sharifulin D,Khairulina Y,Ivanov A,Meschaninova M,Ven'yaminova A,Graifer D,Karpova G

更新日期：2012-04-01 00:00:00

abstract：:Constitutive expression of major histocompatibility complex class II (MHC II) antigens normally occurs in B-lymphocytes and antigen presenting cells of the monocyte/macrophage lineage. However, many malignant tumours and transformed cells express these proteins aberrantly. We demonstrate here that the MHC II DR alpha ...

journal_title：Nucleic acids research

authors： Cox PM,Goding CR

更新日期：1992-09-25 00:00:00

abstract：:Gold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide-nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting tra...

journal_title：Nucleic acids research

authors： Murphy D,Eritja R,Redmond G

更新日期：2004-04-23 00:00:00