The regulatory region of the human plasminogen activator inhibitor type-1 (PAI-1) gene.

abstract：:The locus DXS269 (P20) defines a deletion hotspot in the distal part of the Duchenne Muscular Dystrophy gene. We have cloned over 90 kilobase-pairs of genomic DNA from this region in overlapping cosmids. The use of whole cosmids as probes in a competitive DNA hybridization analysis proves a fast and convenient method ...

journal_title：Nucleic acids research

authors： Blonden LA,den Dunnen JT,van Paassen HM,Wapenaar MC,Grootscholten PM,Ginjaar HB,Bakker E,Pearson PL,van Ommen GJ

更新日期：1989-07-25 00:00:00

abstract：:We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and alpha-fetoprotein genes in the fetal liver (which synthesizes albumin and alpha-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes alpha-fetoprotein), and adult kidney (which synthesizes neither). Active...

journal_title：Nucleic acids research

authors： Kunnath L,Locker J

更新日期：1985-01-11 00:00:00

abstract：:I show that the recognition sequences of Type II restriction systems are correlated with the G + C content of the host bacterial DNA. Almost all restriction systems with G + C rich tetranucleotide recognition sequences are found in species with A + T rich genomes, whereas G + C rich hexanucleotide and octanucleotide r...

journal_title：Nucleic acids research

authors： McClelland M

更新日期：1988-03-25 00:00:00

abstract：:Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against natural transformatio...

journal_title：Nucleic acids research

authors： Dwivedi GR,Sharma E,Rao DN

更新日期：2013-03-01 00:00:00

abstract：:HT1080 cells stably expressing green fluorescent protein (GFP) linked to a 3' terminal AU-rich element (ARE) proved to be a convenient system to study the dynamics of mRNA stability, as changes in mRNA levels are reflected in increased or decreased fluorescence intensity. This study examined whether mRNA stability can...

journal_title：Nucleic acids research

authors： Raineri I,Wegmueller D,Gross B,Certa U,Moroni C

更新日期：2004-02-19 00:00:00

abstract：:In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease-exonuclease-phosphatase domain....

journal_title：Nucleic acids research

authors： Maryati M,Kaur I,Jadhav GP,Olotu-Umoren L,Oveh B,Hashmi L,Fischer PM,Winkler GS

更新日期：2014-03-01 00:00:00

abstract：:A strategy for DNA computing on surfaces using linked sets of 'DNA words' that are short oligonucleotides (16mers) is proposed. The 16mer words have the format 5'-FFFFvvvvvvvvFFFF-3' in which 4-8 bits of data are stored in 8 variable ('v') base locations, and the remaining fixed ('F') base locations are used as a word...

journal_title：Nucleic acids research

authors： Frutos AG,Liu Q,Thiel AJ,Sanner AM,Condon AE,Smith LM,Corn RM

更新日期：1997-12-01 00:00:00

abstract：:MeCP2 is a methyl-CpG binding protein that can repress transcription of nearby genes. In humans, mutations in the MECP2 gene are the major cause of Rett syndrome. By searching expressed sequence tag (EST) databases we have found a novel MeCP2 splice isoform (MeCP2alpha) which encodes a distinct N-terminus. We demonstr...

journal_title：Nucleic acids research

authors： Kriaucionis S,Bird A

更新日期：2004-03-19 00:00:00

abstract：:Insertion of the four major nucleotides at the 5'-side of the anticodon triplet of E. coli tRNAMetf was performed by joining of the half molecules obtained by limited digestion with RNase A and the chemically synthesized tetranucleotide pN-C-A-U using RNA ligase. Insertion of U-U at the 5'-side or A and A-A at the 3'-...

journal_title：Nucleic acids research

authors： Doi T,Yamane A,Matsugi J,Ohtsuka E,Ikehara M

更新日期：1985-05-24 00:00:00

abstract：:Retroviral particles contain a dimeric RNA genome, which serves as template for the generation of double-stranded DNA by reverse transcription. Transfer between RNA strands during DNA synthesis is governed by both sequence similarity between templates and structural features of the dimeric RNA. A kissing hairpin, beli...

journal_title：Nucleic acids research

authors： Mikkelsen JG,Rasmussen SV,Pedersen FS

更新日期：2004-01-09 00:00:00

abstract：:Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...

journal_title：Nucleic acids research

authors： Lemaigre FP,Ace CI,Green MR

更新日期：1993-06-25 00:00:00

abstract：:A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amin...

journal_title：Nucleic acids research

authors： Graham RW,Atkinson T,Kilburn DG,Miller RC Jr,Warren RA

更新日期：1993-10-25 00:00:00

abstract：:The RecQL4 helicase is involved in the maintenance of genome integrity and DNA replication. Mutations in the human RecQL4 gene cause the Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. Mouse models and experiments in human and Xenopus have proven the N-terminal part of RecQL4 to be vital for cell growth. We ...

journal_title：Nucleic acids research

authors： Ohlenschläger O,Kuhnert A,Schneider A,Haumann S,Bellstedt P,Keller H,Saluz HP,Hortschansky P,Hänel F,Grosse F,Görlach M,Pospiech H

更新日期：2012-09-01 00:00:00

abstract：:Proper DNA double-strand break (DSB) repair is essential for maintaining genome integrity. Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism, which introduces mutations at break sites and contributes to chromosomal translocations and telomere fusions, thus driving carcinogenesis. Mitotic kin...

journal_title：Nucleic acids research

authors： Wang H,Qiu Z,Liu B,Wu Y,Ren J,Liu Y,Zhao Y,Wang Y,Hao S,Li Z,Peng B,Xu X

更新日期：2018-11-16 00:00:00

abstract：:Understanding the normal state of human tissue transcriptome profiles is essential for recognizing tissue disease states and identifying disease markers. Recently, the Human Protein Atlas and the FANTOM5 consortium have each published extensive transcriptome data for human samples using Illumina-sequenced RNA-Seq and ...

journal_title：Nucleic acids research

authors： Yu NY,Hallström BM,Fagerberg L,Ponten F,Kawaji H,Carninci P,Forrest AR,Fantom Consortium.,Hayashizaki Y,Uhlén M,Daub CO

更新日期：2015-08-18 00:00:00

abstract：:The Yeast Protein Localization database (YPL.db) contains information about the localization patterns of yeast proteins resulting from microscopic analyses. The data and parameters of the experiments to obtain the localization information, together with images from confocal or video microscopy, are stored in a relatio...

journal_title：Nucleic acids research

authors： Habeler G,Natter K,Thallinger GG,Crawford ME,Kohlwein SD,Trajanoski Z

更新日期：2002-01-01 00:00:00

abstract：:In the haploid genome of Xenopus laevis there are two genes coding for the r-protein L14. It is not known if they are located on the same chromosome. cDNA clones deriving from the transcripts of the two genes have been isolated from an oocyte messenger cDNA bank showing that they are both expressed. We have studied th...

journal_title：Nucleic acids research

authors： Beccari E,Mazzetti P,Mileo A,Bozzoni I,Pierandrei-Amaldi P,Amaldi F

更新日期：1986-10-10 00:00:00

abstract：:Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous w...

journal_title：Nucleic acids research

authors： Houghton M,Easton MA,Stewart AG,Smith JC,Doel SM,Catlin GH,Lewis HM,Patel TP,Emtage JS,Carey NH,Porter AG

更新日期：1980-07-11 00:00:00

abstract：:The Transcription Regulatory Regions Database (TRRD) is a curated database designed for accumulation of experimental data on extended regulatory regions of eukaryotic genes, the regulatory elements they contain, i.e., transcription factor binding sites, promoters, enhancers, silencers, etc., and expression patterns of...

journal_title：Nucleic acids research

authors： Kolchanov NA,Ananko EA,Podkolodnaya OA,Ignatieva EV,Stepanenko IL,Kel-Margoulis OV,Kel AE,Merkulova TI,Goryachkovskaya TN,Busygina TV,Kolpakov FA,Podkolodny NL,Naumochkin AN,Romashchenko AG

更新日期：1999-01-01 00:00:00

abstract：:DNA binding proteins that induce structural changes in DNA are common in both prokaryotes and eukaryotes. Integration host factor (IHF) is a multi-functional DNA binding and bending protein of Escherichia coli that can mediate protein-protein and protein-DNA interactions by bending DNA. Previously we have shown that t...

journal_title：Nucleic acids research

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1996-05-01 00:00:00

abstract：:Electrophoretic mobility shift assays (EMSA) were used to define the regions of the INO1 promoter that interact with factors present in extracts prepared from the yeast, Saccharomyces cerevisae. These experiments identified three different types of protein:DNA complexes that assemble with the INO1 promoter. Formation ...

journal_title：Nucleic acids research

authors： Lopes JM,Henry SA

更新日期：1991-07-25 00:00:00

abstract：:Double stranded cDNA of cucumber pale fruit viroid ( CPFV ) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982] and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence o...

journal_title：Nucleic acids research

authors： Sano T,Uyeda I,Shikata E,Ohno T,Okada Y

更新日期：1984-04-25 00:00:00

abstract：:The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) ...

journal_title：Nucleic acids research

authors： Khare D,Orban J

更新日期：1992-10-11 00:00:00

abstract：:The NusA protein is a universally conserved bacterial transcription elongation factor that binds RNA polymerase (RNAP). When functioning independently, NusA enhances intrinsic termination. Paradoxically, NusA stimulates the function of the N and Q antiterminator proteins of bacteriophage λ. The mechanistic basis for N...

journal_title：Nucleic acids research

authors： Wells CD,Deighan P,Brigham M,Hochschild A

更新日期：2016-06-20 00:00:00

abstract：:A vast number of sweet tasting molecules are known, encompassing small compounds, carbohydrates, d-amino acids and large proteins. Carbohydrates play a particularly big role in human diet. The replacement of sugars in food with artificial sweeteners is common and is a general approach to prevent cavities, obesity and ...

journal_title：Nucleic acids research

authors： Ahmed J,Preissner S,Dunkel M,Worth CL,Eckert A,Preissner R

更新日期：2011-01-01 00:00:00

abstract：:Synchronous plasmodia of Physarum polycephalum in DNA synthesis were pulse-labelled with [oH]- thymidine for time periods of 15 seconds up to 9 minutes, or given a 30 seconds pulse followed by chase periods of 9 minutes up to 6 hours. Sedimentation analysis in alkaline sucrose gradients revealed at least five species ...

journal_title：Nucleic acids research

authors： Funderud S,Haugli F

更新日期：1975-08-01 00:00:00

abstract：:Glutaminyl-tRNA synthetase from Deinococcus radiodurans possesses a C-terminal extension of 215 residues appending the anticodon-binding domain. This domain constitutes a paralog of the Yqey protein present in various organisms and part of it is present in the C-terminal end of the GatB subunit of GatCAB, a partner of...

journal_title：Nucleic acids research

authors： Deniziak M,Sauter C,Becker HD,Paulus CA,Giegé R,Kern D

更新日期：2007-01-01 00:00:00

abstract：:Signal Transducers and Activators of Transcription (STAT) 5A/B regulate cytokine-inducible genes upon binding to GAS motifs. It is not known what percentage of genes with GAS motifs bind to and are regulated by STAT5. Moreover, it is not clear whether genome-wide STAT5 binding is modulated by its concentration. To cla...

journal_title：Nucleic acids research

authors： Zhu BM,Kang K,Yu JH,Chen W,Smith HE,Lee D,Sun HW,Wei L,Hennighausen L

更新日期：2012-05-01 00:00:00

abstract：:We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1s-1. We find that GT-containing oligonucleotides bind much fa...

journal_title：Nucleic acids research

authors： Paes HM,Fox KR

更新日期：1997-08-15 00:00:00

abstract：:The Mouse Genome Database (MGD) focuses on the integration of mapping, homology, polymorphism and molecular data about the laboratory mouse. Detailed descriptions of genes including their chromosomal location, gene function, disease associations, mutant phenotypes, molecular polymorphisms and links to representative s...

journal_title：Nucleic acids research

authors： Blake JA,Richardson JE,Davisson MT,Eppig JT

更新日期：1999-01-01 00:00:00