Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.

abstract：:Recent studies show that the region of DNA brought into close proximity to the fetal gamma-globin genes in deletional forms of hereditary persistence of fetal hemoglobin (HPFH) is selectively hypomethylated (and presumably active) in normal erythroid tissue. This region is normally located approximately 100 kilobases ...

journal_title：Nucleic acids research

authors： Poncz M,Sutton M,Delgrosso K,Schwartz E,Surrey S

更新日期：1987-07-10 00:00:00

abstract：:Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little informati...

journal_title：Nucleic acids research

authors： Babiano R,de la Cruz J

更新日期：2010-08-01 00:00:00

abstract：:Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore, DNA double-strand break signalling has been pinpointed as a key inducer of cellular senescence. Several recent findings s...

journal_title：Nucleic acids research

authors： Grillari J,Katinger H,Voglauer R

更新日期：2007-01-01 00:00:00

abstract：:Apolipoprotein B (apoB) is a major protein component of low density and very low density lipoproteins. Because of its large size and heterogeneity, molecular studies of apoB have been difficult, and its structure and regulation remain poorly understood. We now report the identification of human apoB cDNA clones by ant...

journal_title：Nucleic acids research

authors： Mehrabian M,Schumaker VN,Fareed GC,West R,Johnson DF,Kirchgessner T,Lin HC,Wang XB,Ma YH,Mendiaz E

更新日期：1985-10-11 00:00:00

abstract：:The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA ...

journal_title：Nucleic acids research

authors： White JH,Kilbey BJ,de Vries E,Goman M,Alano P,Cheesman S,McAleese S,Ridley RG

更新日期：1993-08-11 00:00:00

abstract：:Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...

journal_title：Nucleic acids research

authors： Agris PF,Schmidt PG

更新日期：1980-05-10 00:00:00

abstract：:Rad51 protein promotes homologous recombination in eukaryotes. Recombination activities are activated by Rad51 filament assembly on ssDNA. Previous studies of yeast Rad51 showed that His352 occupies an important position at the filament interface, where it could relay signals between subunits and active sites. To inve...

journal_title：Nucleic acids research

authors： Chen J,Villanueva N,Rould MA,Morrical SW

更新日期：2010-08-01 00:00:00

abstract：:LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally he...

journal_title：Nucleic acids research

authors： Rotman G,Itin A,Keshet E

更新日期：1986-01-24 00:00:00

abstract：:Using two primers corresponding to helix 1 and helix 3 regions in the homeodomain, we subjected genomic DNA from Caenorhabditis elegans to amplification by the polymerase chain reaction. Sequence analysis of the amplified products revealed a new homeobox-containing gene, designated ceh-19. This gene was located betwee...

journal_title：Nucleic acids research

authors： Naito M,Kohara Y,Kurosawa Y

更新日期：1992-06-25 00:00:00

abstract：:LocDB is a manually curated database with experimental annotations for the subcellular localizations of proteins in Homo sapiens (HS, human) and Arabidopsis thaliana (AT, thale cress). Currently, it contains entries for 19,604 UniProt proteins (HS: 13,342; AT: 6262). Each database entry contains the experimentally der...

journal_title：Nucleic acids research

authors： Rastogi S,Rost B

更新日期：2011-01-01 00:00:00

abstract：:As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the fam...

journal_title：Nucleic acids research

authors： Massad T,Skaar K,Nilsson H,Damberg P,Henriksson-Peltola P,Haggård-Ljungquist E,Högbom M,Stenmark P

更新日期：2010-11-01 00:00:00

abstract：:The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...

journal_title：Nucleic acids research

authors： Pack GR,Wong L,Prasad CV

更新日期：1986-02-11 00:00:00

abstract：:Not I linking clones contain sequences flanking Not I recognition sites and were previously shown to be tightly associated with CpG islands and genes. To directly assess the value of Not I clones in genome research, high density grids with 50 000 Not I linking clones originating from six representative Not I linking l...

journal_title：Nucleic acids research

authors： Zabarovsky ER,Gizatullin R,Podowski RM,Zabarovska VV,Xie L,Muravenko OV,Kozyrev S,Petrenko L,Skobeleva N,Li J,Protopopov A,Kashuba V,Ernberg I,Winberg G,Wahlestedt C

更新日期：2000-04-01 00:00:00

abstract：:Hepatocyte Nuclear Factor 4 (HNF-4), a liver-enriched orphan receptor of the nuclear receptor superfamily, is required for the expression of a wide variety of liver-specific genes including apoAI. To explore the possibility that site A of the apoAI gene enhancer might also be the target for HNF-4 without the interfere...

journal_title：Nucleic acids research

authors： Fuernkranz HA,Wang Y,Karathanasis SK,Mak P

更新日期：1994-12-25 00:00:00

abstract：:The interaction of antibodies directed toward N6-(delta2-isopentenyl)adenosine, i6Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoassay to quantitate th...

journal_title：Nucleic acids research

authors： Milstone DS,Vold BS,Glitz DG,Shutt N

更新日期：1978-09-01 00:00:00

abstract：:The TWIST1 bHLH transcription factor controls embryonic development and cancer processes. Although molecular and genetic analyses have provided a wealth of data on the role of bHLH transcription factors, very little is known on the molecular mechanisms underlying their binding affinity to the E-box sequence of the pro...

journal_title：Nucleic acids research

authors： Bouard C,Terreux R,Honorat M,Manship B,Ansieau S,Vigneron AM,Puisieux A,Payen L

更新日期：2016-06-20 00:00:00

abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...

journal_title：Nucleic acids research

authors： Arnott S,Bond PJ,Selsing E,Smith PJ

更新日期：1976-10-01 00:00:00

abstract：:Xenopus laevis 5S rRNA isolated from 7S particles or transcribed in vitro is found to adopt two alternative conformations. These two conformers contain different structural elements within the major TF III A binding domain, which, when isolated as RNA fragments, still interact with the transcription factor. Chemical m...

journal_title：Nucleic acids research

authors： Pieler T,Guddat U,Oei SL,Erdmann VA

更新日期：1986-08-11 00:00:00

abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...

journal_title：Nucleic acids research

authors： Kojima T,Takei Y,Ohtsuka M,Kawarasaki Y,Yamane T,Nakano H

更新日期：2005-10-06 00:00:00

abstract：:The Pk-rec gene, encoding a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcussp. KOD1, was expressed in Escherichia coli. The recombinant Pk-REC was purified to homogeneity and was shown to be in a dimeric form. A striking property of the purified recombinant Pk-REC was the unusual DNase activity on ...

journal_title：Nucleic acids research

authors： Rashid N,Morikawa M,Nagahisa K,Kanaya S,Imanaka T

更新日期：1997-02-15 00:00:00

abstract：:Bcg I and Bcg I-like restriction endonucleases cleave double stranded DNA specifically on both sides of their asymmetric recognition sequences which are interrupted by several ambiguous base pairs. Their heterosubunit structure, bifunctionality and stimulation by AdoMet make them different from other classified restri...

journal_title：Nucleic acids research

authors： Vitkute J,Maneliene Z,Petrusyte M,Janulaitis A

更新日期：1997-11-15 00:00:00

abstract：:Expression of a proof-reading deficient form of mitochondrial DNA (mtDNA) polymerase gamma, POLG, causes early death accompanied by features of premature ageing in mouse. However, the mechanism of cellular senescence remains unresolved. In addition to high levels of point mutations of mtDNA, the POLG mutator mouse har...

journal_title：Nucleic acids research

authors： Bailey LJ,Cluett TJ,Reyes A,Prolla TA,Poulton J,Leeuwenburgh C,Holt IJ

更新日期：2009-04-01 00:00:00

abstract：:Genes in the germline (micronuclear) genome of hypotrichous ciliates are interrupted by multiple, short, non-coding, AT-rich sequences called internal eliminated segments, or IESs. During conversion of a micronucleus to a somatic nucleus (macronucleus) after cell mating, all IESs are excised from the germline genes an...

journal_title：Nucleic acids research

authors： Prescott DM

更新日期：1999-03-01 00:00:00

abstract：:Most human genes generate multiple transcript isoforms. The differential expression of these isoforms can help specify cell types. Diverse transcript isoforms arise from the use of alternative transcription start sites, polyadenylation sites and splice sites; however, the relative contribution of these processes to is...

journal_title：Nucleic acids research

authors： Reyes A,Huber W

更新日期：2018-01-25 00:00:00

abstract：:In order to investigate the possible role of RNA secondary structure in determining the efficiency and specificity of mRNA splicing, the structures of sequences at three acceptor splice sites in adenovirus were studied. Transcripts spanning intron-exon junctions were synthesized using SP6 RNA polymerase and analyzed u...

journal_title：Nucleic acids research

authors： Munroe SH

更新日期：1984-11-26 00:00:00

abstract：:The deoxyoligonucleotide 5' AATCGGGCATGGATT (15-mer) was synthesized containing 12 phosphoramidate linkages derived from 2 primary and 2 secondary amines. The oligonucleotides were purified by reverse phase HPLC and characterized by PAGE. The thermal stability of the duplexes derived from these compounds, when hybridi...

journal_title：Nucleic acids research

authors： Froehler B,Ng P,Matteucci M

更新日期：1988-06-10 00:00:00

abstract：:DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exch...

journal_title：Nucleic acids research

authors： Seitz EM,Kowalczykowski SC

更新日期：2006-05-24 00:00:00

abstract：:Decay of (125)I produces a shower of low energy electrons (Auger electrons) that cause strand breaks in DNA in a distance-dependent manner with 90% of the breaks located within 10 bp from the decay site. We studied strand breaks in RNA molecules produced by decay of (125)I incorporated into complementary DNA oligonucl...

journal_title：Nucleic acids research

authors： Gaidamakova EK,Neumann RD,Panyutin IG

更新日期：2002-11-15 00:00:00

abstract：:In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...

journal_title：Nucleic acids research

authors： Okuda K,Shikanai T

更新日期：2012-06-01 00:00:00

abstract：:Clones carrying sequences expressed at altered abundance levels in dunce mutants were isolated by differentially screening a genomic library with cDNA probes representing the RNA population from dunce+ flies and the RNA population from dunce mutant flies. These mutants have an elevated cAMP content, so some isolates p...

journal_title：Nucleic acids research

authors： Yun YD,Davis RL

更新日期：1989-10-25 00:00:00