Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.

Abstract:

:LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally heterogenous VL30 LTRs displayed highly variable promoter/enhancer activities. The most active VL30 LTR (designated VL3) promoted CAT activity to levels six-fold higher than the LTR of the strongly transforming retrovirus, MSV. This VL30 transcription unit, containing a unique U3 region, was further characterized by S1 nuclease mapping. VL3 LTR functioned as an enhancer in CAT constructs containing a SV40 promoter. In addition, a defined U3 segment was shown to augment expression of CAT in an orientation independent manner. VL3 LTR also served as an efficient promoter and enhancer in heterologous monkey cells. These results suggest that certain resident LTRs possess promoter and enhancer capacities greater than those possessed by LTRs of infectious retroviruses.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Rotman G,Itin A,Keshet E

doi

10.1093/nar/14.2.645

subject

Has Abstract

pub_date

1986-01-24 00:00:00

pages

645-58

issue

2

eissn

0305-1048

issn

1362-4962

journal_volume

14

pub_type

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