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The gene encoding DNA polymerase alpha from Plasmodium falciparum.

Abstract：

:The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA polymerases. The predicted protein also contains sequences which are reminiscent of Plasmodium proteins but absent from other DNA polymerases. These include four blocks of additional amino acids interspersed with the conserved motifs of the DNA polymerases, four asparagine rich sequences and a novel carboxy-terminal extension. Repetitive sequences similar to those found in other malarial proteins are also present. cDNA-directed PCR was used to establish the presence of these features in the approximately 7kb mRNA. The coding sequence contains a single intron. The gene for DNAPol alpha is located on chromosome 4 and is transcribed in both asexual and sexual erythrocytic stages of the parasite.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

White JH,Kilbey BJ,de Vries E,Goman M,Alano P,Cheesman S,McAleese S,Ridley RG

doi

10.1093/nar/21.16.3643

subject

Has Abstract

pub_date

1993-08-11 00:00:00

pages

3643-6

issue

16

eissn

0305-1048

issn

1362-4962

journal_volume

21

pub_type

杂志文章

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