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Evidence for cross-linking DNA by bis-intercalators with rigid and extended linkers is provided by knotting and catenation.

Abstract：

:A new series of DNA bis-intercalators is reported in which acridine moieties are connected by rigid and extended pyridine-based linkers of varied length. Cross-linking of DNA by bis-intercalation is inferred from the unwinding and folding of linear DNA induced by the compounds; after ligation and removal of the bis-intercalator, superhelical circles, catenanes and knots that bear a residual imprint of the bis-intercalator are observed. These novel bis-intercalators are of interest because they can be used to probe the spatial organization of DNA, especially near sites of replication, recombination or topoisomerase action where two duplexes must be in close proximity. Preliminary results on the effects of the various compounds on the cloning efficiency of bacteria and replication by permeabilized human cells are also presented.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Annan NK,Cook PR,Mullins ST,Lowe G

doi

10.1093/nar/20.5.983

keywords:

subject

Has Abstract

pub_date

1992-03-11 00:00:00

pages

983-90

issue

5

eissn

0305-1048

issn

1362-4962

journal_volume

20

pub_type

杂志文章

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