Abstract：
:These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and Cfo I, are reduced with Z DNA as compared to B DNA. The ability of both restriction endonucleases to react with an aggregate form of Z DNA (Z* DNA) is found to depend upon how the Z* DNA is formed. When Z* DNA is induced by low concentrations of Co (50 microM), the endonucleases remain active. In the presence of 100 microM Co, which causes increased aggregation, the endonucleases are inactive. The Hha I DNA methyltransferase reacts at equal rates with the B, Z and low cobalt Z* forms and at a greatly reduced rate with the high cobalt Z* form. These results are significantly different than those observed with Z form dGdC tracts inserted into circular DNA molecules.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Soslau G,Parker J,Nelson JWdoi
10.1093/nar/14.18.7237subject
Has Abstractpub_date
1986-09-25 00:00:00pages
7237-52issue
18eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract：:Activating sequence factor 1 (ASF-1) is a nuclear DNA-binding activity that is found in monocots and dicots. It interacts with several TGACG-containing elements that have been characterized from viral and T-DNA genes, the prototypes of which are the as-1 element of the CaMV 35S promoter and the ocs element from the oc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.18.3778
更新日期：1995-09-25 00:00:00
abstract：:CCG repeats are highly over-represented in exons of the human genome. Usually they are located in the 5' UTR but are also abundant in translated sequences. The CCG repeats are associated with three tri-nucleotide repeat disorders: Huntington's disease, myotonic dystrophy type 1 and chromosome X-linked mental retardati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks557
更新日期：2012-09-01 00:00:00
abstract：:Mitochondrial transcription factor A (mtTFA/TFAM) is a nucleus-encoded, high-mobility-group-box (HMG-box) protein that regulates transcription of the mitochondrial genome by specifically recognizing light-strand and heavy-strand promoters (LSP, HSP1). TFAM also binds mitochondrial DNA in a non-sequence specific (NSS) ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr787
更新日期：2012-01-01 00:00:00
abstract：:The advent of jellyfish green fluorescent protein and its spectral variants, together with promising new fluorescent proteins from other classes of the Cnidarian phylum (coral and anemones), has greatly enhanced and promises to further boost the detection and localization of proteins in cell biology. It has been less ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/29.5.1013
更新日期：2001-03-01 00:00:00
abstract：:Aside from classical loops among G-quadruplexes, the unique leaped V-shape scaffold spans over three G-tetrads, without any intervening residues. This scaffold enables a sharp reversal of two adjacent strand directions and simultaneously participates in forming the G-tetrad core. These features make this scaffold itse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1167
更新日期：2019-02-20 00:00:00
abstract：:A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.3.599
更新日期：1990-02-11 00:00:00
abstract：:Based on the secondary structural model of MS2 RNA, it is shown that, in base-pairing regions of the RNA, there is a bias in the use of synonymous codons which favours C and/or G over U and/or A in the third codon positions, and that in non-pairing regions, there is an opposite bias which favours U and/or A over C and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.7.2073
更新日期：1979-12-11 00:00:00
abstract：:We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a gua...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.15.5093
更新日期：1983-08-11 00:00:00
abstract：:The Drosophila mophogenetic protein Bicoid (Bcd) can activate transcription in a concentration-dependent manner in embryos. It contains a self-inhibitory domain that can interact with the co-repressor Sin3A. In this report, we study a Bcd mutant, Bcd(A57-61), which has a strengthened self-inhibitory function and is un...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki691
更新日期：2005-07-19 00:00:00
abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.14.4793
更新日期：1983-07-25 00:00:00
abstract：:Approaches to characterize the nucleic acid-binding properties of drugs and druglike small molecules are crucial to understanding the behavior of these compounds in cellular systems. Here, we use a Small Molecule Microarray (SMM) profiling approach to identify the preferential interaction between chlorhexidine, a wide...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky084
更新日期：2018-04-06 00:00:00
abstract：:We have examined the absorption and circular dichroism spectra of intact Penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded RNA. The absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded RNA and free viral protein, ind...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.1.175
更新日期：1983-01-11 00:00:00
abstract：:For the first time mosaic nucleic acids composed of 50% RNA and 50% DNA can be obtained as transcripts with T7 RNA polymerase. Two NTPs could be replaced simultaneously in a transcription reaction. This means more than 40 deoxynucleotides were inserted in one transcript. Previously, a maximum of two deoxynucleotides c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.11.1845
更新日期：1995-06-11 00:00:00
abstract：:Infrared spectroscopy was used to identify hydration-sensitive structural differences between single- stranded phosphorothioate (PS) and phosphodiester (PO) oligodeoxyribonucleotides. Spectra were recorded in the mid-infrared region, 500-1800 cm-1, at relative humidities between 0 and 98%; the PS and PO spectra are su...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.16.3261
更新日期：1996-08-15 00:00:00
abstract：:The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort among model plant genome databases and plant researchers that aims to create, maintain and facilitate the use of a controlled vocabulary (ontology) for plants. The ontology allows users to ascribe attributes of plant structure ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm908
更新日期：2008-01-01 00:00:00
abstract：:The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB protein is 3543 nucleotides long and would encode a polypeptide of 1180 amino acids with a calculated molecular weight of 13...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.21.8573
更新日期：1986-11-11 00:00:00
abstract：:The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.5.1539
更新日期：1977-01-01 00:00:00
abstract：:Short interfering RNAs (siRNAs) are valuable reagents for sequence-specific inhibition of gene expression via the RNA interference (RNAi) pathway. Although it has been proposed that the relative thermodynamic stability at the 5'-ends of siRNAs plays a crucial role in siRNA strand selection, we demonstrate here that a ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn584
更新日期：2008-10-01 00:00:00
abstract：:Synthetic deoxyoligonucleotides have been 5'-aminoalkylated at the end of step-wise synthesis on the polymer support. This was achieved through the activation of the 5'-hydroxyl group as its 5'-imidazolyl derivative using carbonyldiimidazole, which was subsequently displaced with hexamethylene diamine to yield the tit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.20.7985
更新日期：1986-10-24 00:00:00
abstract：:Glucocorticoid (GC) signaling synchronizes the circadian rhythm of individual peripheral cells and induces the expression of circadian genes, including Period1 (Per1) and Period2 (Per2). However, no GC response element (GRE) has been reported in the Per2 promoter region. Here we report the molecular mechanisms of Per2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt307
更新日期：2013-07-01 00:00:00
abstract：:Post-translational modifications (PTMs) play a key role in numerous cellular processes by directly affecting structure, dynamics and interaction networks of target proteins. Despite their importance, our understanding of protein PTMs at the atomistic level is still largely incomplete. Molecular dynamics (MD) simulatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt416
更新日期：2013-07-01 00:00:00
abstract：:The Network of Cancer Genes (NCG) collects and integrates data on 736 human genes that are mutated in various types of cancer. For each gene, NCG provides information on duplicability, orthology, evolutionary appearance and topological properties of the encoded protein in a comprehensive version of the human protein-p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp957
更新日期：2010-01-01 00:00:00
abstract：:The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.5.1449
更新日期：1977-01-01 00:00:00
abstract：:Poly (ADP-ribose) polymerase 1 (PARP1) has emerged as an attractive target for cancer therapy due to its key role in DNA repair processes. Inhibition of PARP1 in BRCA-mutated cancers has been observed to be clinically beneficial. Recent genome-mapping experiments have identified a non-canonical G-quadruplex-forming se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1179
更新日期：2019-02-20 00:00:00
abstract：:Naturally occurring RNAs are known to exhibit a high degree of modularity, whereby specific structural modules (or motifs) can be mixed and matched to create new molecular architectures. The modular nature of RNA also affords researchers the ability to characterize individual structural elements in controlled syntheti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz304
更新日期：2019-07-09 00:00:00
abstract：:Among four types of bacterial restriction enzymes that cleave a foreign DNA depending on its methylation status, type I enzymes composed of three subunits are interesting because of their unique DNA cleavage and translocation mechanisms performed by the restriction subunit (HsdR). The elucidated N-terminal fragment st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp603
更新日期：2009-11-01 00:00:00
abstract：:Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. Cellular PAcP functions as a neutral protein tyrosine phosphatase and is involved in regulating androgen-promoted prostate cancer cell proliferation. Despite the fact that the promoter of the PAcP gene has been cloned, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh677
更新日期：2004-07-07 00:00:00
abstract：:Structural information on interacting proteins is important for understanding life processes at the molecular level. Genome-wide docking database is an integrated resource for structural studies of protein-protein interactions on the genome scale, which combines the available experimental data with models obtained by ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp944
更新日期：2010-01-01 00:00:00
abstract：:Understanding the molecular mechanisms that influence transposable element target site preferences is a fundamental challenge in functional and evolutionary genomics. Large-scale transposon insertion projects provide excellent material to study target site preferences in the absence of confounding effects of post-inse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn563
更新日期：2008-11-01 00:00:00
abstract：:During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw302
更新日期：2016-07-08 00:00:00
