A simple and efficient procedure for the synthesis of 5'-aminoalkyl oligodeoxynucleotides.

abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is Europe's primary resource for nucleotide sequence information. With the growing volume and diversity of public sequencing data comes the need for increased sophistication in data organisation, presentation and search services so as to maximise its disc...

journal_title：Nucleic acids research

authors： Silvester N,Alako B,Amid C,Cerdeño-Tárraga A,Cleland I,Gibson R,Goodgame N,Ten Hoopen P,Kay S,Leinonen R,Li W,Liu X,Lopez R,Pakseresht N,Pallreddy S,Plaister S,Radhakrishnan R,Rossello M,Senf A,Smirnov D,Toribio A

更新日期：2015-01-01 00:00:00

abstract：:Using a bio-oligo pull-down DNA-binding assay we investigated the binding capacity of endogenous, DNA damage-induced p53 in human diploid fibroblasts to several p53-responsive elements (REs) present in p53-regulated genes. During the course of p53 accumulation, we observed a decrease in p53 binding to the GADD45 but n...

journal_title：Nucleic acids research

authors： Buzek J,Latonen L,Kurki S,Peltonen K,Laiho M

更新日期：2002-06-01 00:00:00

abstract：:To generate long arrays of nucleosomes within a topologically defined chromatin domain we have assembled minichromosomes on negatively supercoiled plasmid DNA with extracts from Drosophila preblastoderm embryos. These minichromosomes are dynamic substrates for energy-dependent nucleosome remodeling machines that facil...

journal_title：Nucleic acids research

authors： Venditti P,Di Croce L,Kauer M,Blank T,Becker PB,Beato M

更新日期：1998-08-15 00:00:00

abstract：:Spectroscopic studies of the complexes of double-stranded (ds) DNA with the polymethylene-amine linked heterodimers thiazole orange-thiazole blue, thiazole orange-ethidium, and fluorescein-ethidium, in each case show efficient energy transfer from donor to acceptor chromophores (Benson, S.C., Singh, P. and Glazer, A.N...

journal_title：Nucleic acids research

authors： Benson SC,Mathies RA,Glazer AN

更新日期：1993-12-11 00:00:00

abstract：:The frequencies of occurrence of four bases in the first, second and third codon positions and in the total coding sequences have been calculated by the codon usage table published in 1990 by Ikemura et al. The distribution of frequencies are further analysed in detail by a graphic technique presented recently by us. ...

journal_title：Nucleic acids research

authors： Zhang CT,Zhang R

更新日期：1991-11-25 00:00:00

abstract：:RNA-based therapeutics, i.e. the utilization of synthetic RNA molecules to alter cellular functions, have the potential to address targets which are currently out of scope for traditional drug design pipelines. This potential however hinges on the ability to selectively deliver and internalize therapeutic RNAs into ce...

journal_title：Nucleic acids research

authors： Wang Y,Hoinka J,Liang Y,Adamus T,Swiderski P,Przytycka TM

更新日期：2018-09-19 00:00:00

abstract：:Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these...

journal_title：Nucleic acids research

authors： Xie Y,Rosser JM,Thompson TL,Boeke JD,An W

更新日期：2011-02-01 00:00:00

abstract：:GGRNA (http://GGRNA.dbcls.jp/) is a Google-like, ultrafast search engine for genes and transcripts. The web server accepts arbitrary words and phrases, such as gene names, IDs, gene descriptions, annotations of gene and even nucleotide/amino acid sequences through one simple search box, and quickly returns relevant Re...

journal_title：Nucleic acids research

authors： Naito Y,Bono H

更新日期：2012-07-01 00:00:00

abstract：:Influenza viruses have a segmented viral RNA (vRNA) genome, which is replicated by the viral RNA-dependent RNA polymerase (RNAP). Replication initiates on the vRNA 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the synthesis of new vRNA. RNAP structures show the 3' termi...

journal_title：Nucleic acids research

authors： Robb NC,Te Velthuis AJ,Wieneke R,Tampé R,Cordes T,Fodor E,Kapanidis AN

更新日期：2016-12-01 00:00:00

abstract：:The primary function of microRNAs (miRNAs) is to maintain cell homeostasis. In cancerous tissues miRNAs' expression undergo drastic alterations. In this study, we use miRNA expression profiles from The Cancer Genome Atlas of 24 cancer types and 3 healthy tissues, collected from >8500 samples. We seek to classify the c...

journal_title：Nucleic acids research

authors： Rasnic R,Linial N,Linial M

更新日期：2017-05-19 00:00:00

abstract：:We describe LincSNP 2.0 (http://bioinfo.hrbmu.edu.cn/LincSNP), an updated database that is used specifically to store and annotate disease-associated single nucleotide polymorphisms (SNPs) in human long non-coding RNAs (lncRNAs) and their transcription factor binding sites (TFBSs). In LincSNP 2.0, we have updated the ...

journal_title：Nucleic acids research

authors： Ning S,Yue M,Wang P,Liu Y,Zhi H,Zhang Y,Zhang J,Gao Y,Guo M,Zhou D,Li X,Li X

更新日期：2017-01-04 00:00:00

abstract：:The cDNA encoding Chinese hamster O6-alkylguanine-DNA-alkyltransferase (ATase) has been isolated from a library prepared from RNA isolated from V79 lung fibroblasts which had an upregulated level of this repair activity following stepwise selection with a chloroethylating agent (1, 2). Expression of the cDNA in E. col...

journal_title：Nucleic acids research

authors： Rafferty JA,Elder RH,Watson AJ,Cawkwell L,Potter PM,Margison GP

更新日期：1992-04-25 00:00:00

abstract：:Folding of group II introns is characterized by a first slow compaction of domain 1 (D1) followed by the rapid docking of other domains to this scaffold. D1 compaction initiates in a small subregion encompassing the κ and ζ elements. These two tertiary elements are also the major interaction sites with domain 5 to for...

journal_title：Nucleic acids research

authors： Donghi D,Pechlaner M,Finazzo C,Knobloch B,Sigel RK

更新日期：2013-02-01 00:00:00

abstract：:Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone...

journal_title：Nucleic acids research

authors： Gabsalilow L,Schierling B,Friedhoff P,Pingoud A,Wende W

更新日期：2013-04-01 00:00:00

abstract：:The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation bet...

journal_title：Nucleic acids research

authors： Suda T,Mishima Y,Takayanagi K,Asakura H,Odani S,Kominami R

更新日期：1996-12-01 00:00:00

abstract：:Insertion of the four major nucleotides at the 5'-side of the anticodon triplet of E. coli tRNAMetf was performed by joining of the half molecules obtained by limited digestion with RNase A and the chemically synthesized tetranucleotide pN-C-A-U using RNA ligase. Insertion of U-U at the 5'-side or A and A-A at the 3'-...

journal_title：Nucleic acids research

authors： Doi T,Yamane A,Matsugi J,Ohtsuka E,Ikehara M

更新日期：1985-05-24 00:00:00

abstract：:The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcrip...

journal_title：Nucleic acids research

authors： Arnosti DN,Preston CM,Hagmann M,Schaffner W,Hope RG,Laughlan G,Luisi BF

更新日期：1993-12-11 00:00:00

abstract：:DNA bearing lambda operator sequences was methylated by dimethyl sulfate (DMS) in the presence or absence of lambda repressor. Under the experimental conditions, DMS methylates only the purine residues. The presence of lambda repressor affects only the methylation of certain G residues in the operators. Repressor bloc...

journal_title：Nucleic acids research

authors： Humayun Z,Kleid D,Ptashne M

更新日期：1977-01-01 00:00:00

abstract：:ArrayExpress is a public repository for microarray data that supports the MIAME (Minimum Information About a Microarray Experiment) requirements and stores well-annotated raw and normalized data. As of November 2004, ArrayExpress contains data from approximately 12,000 hybridizations covering 35 species. Data can be s...

journal_title：Nucleic acids research

authors： Parkinson H,Sarkans U,Shojatalab M,Abeygunawardena N,Contrino S,Coulson R,Farne A,Lara GG,Holloway E,Kapushesky M,Lilja P,Mukherjee G,Oezcimen A,Rayner T,Rocca-Serra P,Sharma A,Sansone S,Brazma A

更新日期：2005-01-01 00:00:00

abstract：:The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational effi...

journal_title：Nucleic acids research

authors： Gallie DR,Feder JN,Schimke RT,Walbot V

更新日期：1991-09-25 00:00:00

abstract：:The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...

journal_title：Nucleic acids research

authors： Ren Q,Au HH,Wang QS,Lee S,Jan E

更新日期：2014-08-01 00:00:00

abstract：:Using two primers corresponding to helix 1 and helix 3 regions in the homeodomain, we subjected genomic DNA from Caenorhabditis elegans to amplification by the polymerase chain reaction. Sequence analysis of the amplified products revealed a new homeobox-containing gene, designated ceh-19. This gene was located betwee...

journal_title：Nucleic acids research

authors： Naito M,Kohara Y,Kurosawa Y

更新日期：1992-06-25 00:00:00

abstract：:We have reported that an upstream stimulatory factor (USF) binding site is functional in transcription of the heme oxygenase-1 gene. In this study, we examined the role of USF in the induced state. By transient expression analyses with the chloramphenicol acetyl-transferase gene, we found that the USF binding site pla...

journal_title：Nucleic acids research

authors： Maeshima H,Sato M,Ishikawa K,Katagata Y,Yoshida T

更新日期：1996-08-01 00:00:00

abstract：:The beta protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been proposed that the DNA region to which the beta recombinase binds to mediate recombination includes a promoter from which o...

journal_title：Nucleic acids research

authors： Rojo F,Alonso JC

更新日期：1994-05-25 00:00:00

abstract：:We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the human papillomavirus E6...

journal_title：Nucleic acids research

authors： Liu WL,Stevenson M,Seymour LW,Fisher KD

更新日期：2009-01-01 00:00:00

abstract：:Genomic DNA of African trypanosomes contains a hypermodified thymidine residue termed base J (beta-d-glucosyl-HOMedU). This modified base is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. The base is synthesized in a two-step pathway. Initially,...

journal_title：Nucleic acids research

authors： Cliffe LJ,Kieft R,Southern T,Birkeland SR,Marshall M,Sweeney K,Sabatini R

更新日期：2009-04-01 00:00:00

abstract：:Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complement...

journal_title：Nucleic acids research

authors： Takahashi M,Nagai C,Hatakeyama H,Minakawa N,Harashima H,Matsuda A

更新日期：2012-07-01 00:00:00

abstract：:ADGO 2.0 is a web-based tool that provides composite interpretations for microarray data comparing two sample groups as well as lists of genes from diverse sources of biological information. Some other tools also incorporate composite annotations solely for interpreting lists of genes but usually provide highly redund...

journal_title：Nucleic acids research

authors： Chi SM,Kim J,Kim SY,Nam D

更新日期：2011-07-01 00:00:00

abstract：:The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...

journal_title：Nucleic acids research

authors： Reinert KE,Geller D,Stutter E

更新日期：1981-05-25 00:00:00

abstract：:The emergence of massively parallel sequencing technology has revolutionized microbial profiling, allowing the unprecedented comparison of microbial diversity across time and space in a wide range of host-associated and environmental ecosystems. Although the high-throughput nature of such methods enables the detection...

journal_title：Nucleic acids research

authors： Amir A,Zeisel A,Zuk O,Elgart M,Stern S,Shamir O,Turnbaugh PJ,Soen Y,Shental N

更新日期：2013-12-01 00:00:00