Abstract:
:The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation between DNA containing the (GGA/TCC)11 repeat and oligonucleotides of d(GGA)11 probably due to G:G base pairing. The activity is to reduce association time and requirements of Mg2+ and oligonucleotide concentrations. The HMG box of SRY, the protein determining male-sex differentiation, also has the activity, suggesting that it is not restricted to the HMG-box domains derived from HMG1/2 but is common to those from other members of the HMG-box family of proteins. Interestingly, the box-AB and box-B of HMG1 bend DNA containing the repeat, but SRY fails to bend in a circularization assay. The difference suggests that the two activities of association-promotion and DNA bending are distinct. These results suggest that the HMG-box domain has a novel activity of promoting the association between GGA repeats which might be involved in higher-order architecture of chromatin.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Suda T,Mishima Y,Takayanagi K,Asakura H,Odani S,Kominami Rdoi
10.1093/nar/24.23.4733subject
Has Abstractpub_date
1996-12-01 00:00:00pages
4733-40issue
23eissn
0305-1048issn
1362-4962pii
6w0150journal_volume
24pub_type
杂志文章abstract::The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays a role in transcription and RNA processing. Yeast ESS1, a peptidyl-prolyl cis/trans isomerase, is involved in RNA processing and can associate with the CTD. Using several types of assays we could not find any evidence of an effect of Pin1, the...
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pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:1987-01-12 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1992-07-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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更新日期:1992-07-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1997-08-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2015-05-19 00:00:00
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