Abstract:
:The beta protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been proposed that the DNA region to which the beta recombinase binds to mediate recombination includes a promoter from which orf alpha and the beta gene are transcribed. We have determined the sites at which transcription of the orf alpha and the beta gene initiates in vitro and we have demonstrated that highly purified beta recombinase acts as a repressor of its own synthesis. The promoters are located within the beta recombinase binding site, which we have defined previously. The binding of the beta recombinase to its target site does not seem to exclude RNA polymerase from the promoter, despite the overlapping of their binding sites. Therefore, it is likely that the beta recombinase does not repress transcription by a mere steric hindrance on RNA polymerase binding.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rojo F,Alonso JCdoi
10.1093/nar/22.10.1855subject
Has Abstractpub_date
1994-05-25 00:00:00pages
1855-60issue
10eissn
0305-1048issn
1362-4962journal_volume
22pub_type
杂志文章abstract::It is a central issue to elucidate the new type of molecular recognition accompanied by a global structural change of a molecule upon binding to its targets. Here we investigate the driving force for the binding of R12 (a ribonucleic acid aptamer) and P16 (a partial peptide of a prion protein) during which P16 exhibit...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-07-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/nar/23.21.4234
更新日期:1995-11-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-10-27 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-09-26 00:00:00
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journal_title:Nucleic acids research
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更新日期:2003-07-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx487
更新日期:2017-07-07 00:00:00
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pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00