A diminutive and specific RNA binding site for L-tryptophan.

Abstract:

:Selection for amino acid affinity by elution of RNAs from tryptophan-Sepharose using free L-tryptophan evokes one sequence predominantly (K(D) = 12 microM), a symmetrical internal loop of 3 nt per side. Though we have also isolated larger sequences with affinity for tryptophan, successively squeezed selection in randomized tracts of 70, 60, 40, 20 and 17 nt show that this internal loop is the simplest sequence that can meet the column affinity selection. From sequence variation in approximately 50 independent isolates, only 26 bits of information are required to describe this loop (equivalent to only 13 fully conserved nucleotides). Thus, it is among the simplest amino acid binding sites known, as well as selective among hydrophobic side chains. Among site sequences defined as essential to affinity by conservation, protection and modification-interference, there is a recurring CCA sequence (a tryptophan anticodon triplet) which apparently forms one side of the binding site. Such conserved juxtaposition of tryptophan with a cognate coding triplet supports a stereochemical origin for the genetic code.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Majerfeld I,Yarus M

doi

10.1093/nar/gki861

keywords:

subject

Has Abstract

pub_date

2005-09-25 00:00:00

pages

5482-93

issue

17

eissn

0305-1048

issn

1362-4962

pii

33/17/5482

journal_volume

33

pub_type

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