Abstract：
:During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We have cloned a new RNA helicase gene, designated DBP2 (DEAH-boxprotein), homologous to the Schizosaccaromyces pombe cdc28(+)/prp8(+) gene involved in pre-mRNA splicing and cell cycle progression. The full-length DBP2 contains 3400 nucleotides and codes for a protein of 1041 amino acids with a calculated mol. wt of 119 037 Da. Transfection experiments demonstrated that the GFP-DBP2 gene product, transiently expressed in HeLa cells, was localized in the nucleus. The DBP2 gene was mapped by FISH to the MHC region on human chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Because the expression of DBP2 gene in S.pombe prp8 mutant cells partially rescued the temperature-sensitive phenotype, we conclude that DBP2 is a functional human homolog of the fission yeast Cdc28/Prp8 protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Imamura O,Saiki K,Tani T,Ohshima Y,Sugawara M,Furuichi Ydoi
10.1093/nar/26.9.2063subject
Has Abstractpub_date
1998-05-01 00:00:00pages
2063-8issue
9eissn
0305-1048issn
1362-4962pii
gkb369journal_volume
26pub_type
杂志文章abstract：:Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn918
更新日期：2009-02-01 00:00:00
abstract：:The specific DNA binding ligand netropsin selectively blocks dA-dT base pairs in clusters containing two or more consecutive thymine residues at the dNAase I cleavage sites of DNA. Using CD and UV absorption measurements it is shown, that at various ratios of netropsin to nucleotide concentrations and even at satuatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.13.2999
更新日期：1980-07-11 00:00:00
abstract：:By successively assembling genetic parts such as BioBrick according to grammatical models, complex genetic constructs composed of dozens of functional blocks can be built. However, usually every category of genetic parts includes a few or many parts. With increasing quantity of genetic parts, the process of assembling...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx859
更新日期：2017-12-15 00:00:00
abstract：:Reverse gyrase is a peculiar DNA topoisomerase, specific of hyperthermophilic Archaea and Bacteria, which has the unique ability of introducing positive supercoiling into DNA molecules. Although the function of the enzyme has not been established directly, it has been suggested to be involved in DNA protection and rep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl115
更新日期：2006-04-14 00:00:00
abstract：:Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibrobla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz338
更新日期：2019-07-09 00:00:00
abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx500
更新日期：2017-08-21 00:00:00
abstract：:A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.19.4395
更新日期：1998-10-01 00:00:00
abstract：:GenBank(®) (http://www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for over 300 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing proj...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1216
更新日期：2015-01-01 00:00:00
abstract：:IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm117
更新日期：2007-01-01 00:00:00
abstract：:With recent advancements in techniques for cellular data acquisition, information on cellular processes has been increasing at a dramatic rate. Visualization is critical to analyzing and interpreting complex information; representing cellular processes or pathways is no exception. VISIBIOweb is a free, open-source, we...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq352
更新日期：2010-07-01 00:00:00
abstract：:Proteins interacting with pre-mRNAs during early stages of spliceosome formation in a HeLa nuclear extract were investigated by photochemical RNA-protein crosslinking. The level of protein crosslinking to a beta-globin pre-mRNA was positively correlated with the presence of an intron. Proteins of 110,000, 59,000 and 3...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.11.3307
更新日期：1990-06-11 00:00:00
abstract：:Differences between species have been suggested to largely reside in the network of connections among the genes. Nevertheless, the rate at which these connections evolve has not been properly quantified. Here, we measure the extent to which co-regulation between pairs of genes is conserved over large phylogenetic dist...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh815
更新日期：2004-09-07 00:00:00
abstract：:In 2.5 x 10(-4)M EDTA buffer, the derivative melting curve of calf thymus DNA shows a major band at 47 degrees with a shoulder at about 54 degrees . The fraction of melting area of this shoulder is about 13%. For reconstituted polylysine-calf thymus DNA complexes, in addition to the melting of free DNA regions at abou...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.2.257
更新日期：1974-02-01 00:00:00
abstract：:Remodelling protein nucleic acid interfaces is an important biological task, which is often carried out by nucleic acid stimulated ATPases of the Swi2/Snf2 superfamily. Here we study the mechano-chemical cycle of such an ATPase, namely the catalytic domain of the Sulfolobus solfataricus Rad54 homologue (SsoRad54cd), b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn040
更新日期：2008-04-01 00:00:00
abstract：:Mitochondrial DNA from human tissue culture cells contains heterogeneous sequences located within a previously identified, evolutionarily conserved region termed CSB-2. CSB-2 is located near the origin of heavy-strand mitochondrial DNA synthesis and the major transcriptional promoters for each strand of human mitochon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.22.8093
更新日期：1985-11-25 00:00:00
abstract：:The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional activity of, and the protein-DNA interactions that occur within, the proximal region of the human beta-actin promoter. Eff...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.24.6913
更新日期：1991-12-25 00:00:00
abstract：:The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.2.307
更新日期：1991-01-25 00:00:00
abstract：:The Deciphering the Mechanisms of Developmental Disorders (DMDD) consortium is a research programme set up to identify genes in the mouse, which if mutated (or knocked-out) result in embryonic lethality when homozygous, and initiate the study of why disruption of their function has such profound effects on embryo deve...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1138
更新日期：2016-01-04 00:00:00
abstract：:Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.23.6369
更新日期：1981-12-11 00:00:00
abstract：:The J-binding protein 1 (JBP1) is essential for biosynthesis and maintenance of DNA base-J (β-d-glucosyl-hydroxymethyluracil). Base-J and JBP1 are confined to some pathogenic protozoa and are absent from higher eukaryotes, prokaryotes and viruses. We show that JBP1 recognizes J-containing DNA (J-DNA) through a 160-res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr125
更新日期：2011-07-01 00:00:00
abstract：:Intrinsic energetic and solvation factors contributing to the unusual structural and biochemical properties of N3'-phosphoramidate DNA analogs have been re-examined using a combination of quantum mechanical and molecular dynamics methods. Evaluation of the impact of the N3'-H substitution was performed via comparison ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.15.3219
更新日期：2001-08-01 00:00:00
abstract：:A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.11.4683
更新日期：1986-06-11 00:00:00
abstract：:Nitrated polycyclic aromatic hydrocarbons are common environmental pollutants, of which many are mutagenic and carcinogenic. 1-Nitropyrene is the most abundant nitrated polycyclic aromatic hydrocarbon, which causes DNA damage and is carcinogenic in experimental animals. Error-prone translesion synthesis of 1-nitropyre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1296
更新日期：2013-02-01 00:00:00
abstract：:The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.1.222
更新日期：1997-01-01 00:00:00
abstract：:Organisms growing at elevated temperatures face the challenge of maintaining the integrity of their genetic materials. Archaea possess unique chromatin proteins for gene organization and information processing. We present the solution structure of Sso7c4 from Sulfolobus solfataricus, which has a homodimeric DNA-bindin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr283
更新日期：2011-08-01 00:00:00
abstract：:The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and mai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks968
更新日期：2013-01-07 00:00:00
abstract：:CS23D (chemical shift to 3D structure) is a web server for rapidly generating accurate 3D protein structures using only assigned nuclear magnetic resonance (NMR) chemical shifts and sequence data as input. Unlike conventional NMR methods, CS23D requires no NOE and/or J-coupling data to perform its calculations. CS23D ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn305
更新日期：2008-07-01 00:00:00
abstract：:The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.ed...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki038
更新日期：2005-01-01 00:00:00
abstract：:The complete sequences of three kinetoplast DNA minicircles (B4, D3 and D12) from Leishmania tarentolae are reported. All L. tarentolae minicircles encode single gRNAs localized within the variable region approximately 150 bp from the conserved region. The 5' termini and tentative 3' termini of the new gRNAs were dete...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.22.6277
更新日期：1991-11-25 00:00:00
abstract：:Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.e12
更新日期：2002-02-01 00:00:00
