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Protection of (dA.dT) cluster regions in the DNAase I cleavage of DNA by specific interaction with netropsin.

Abstract：

:The specific DNA binding ligand netropsin selectively blocks dA-dT base pairs in clusters containing two or more consecutive thymine residues at the dNAase I cleavage sites of DNA. Using CD and UV absorption measurements it is shown, that at various ratios of netropsin to nucleotide concentrations and even at satuation of ligand interaction the enzyme cuts along regions containing dG-dC pairs sandwiched between dA-dT pairs. This follows a slow kinetics and is associated with a release of netropsin from those segments. These facts suggests the usefulness of the partial protection of certain DNA sequences in DNAase I cleavage sites in producing DNA fragments in structural studies of the genome. A possible interpretation of the effect of netropsin binding on the enzymatic hydrolysis of phosphodiester bonds of the helix is discussed.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Zimmer C,Luck G,Nüske R

doi

10.1093/nar/8.13.2999

subject

Has Abstract

pub_date

1980-07-11 00:00:00

pages

2999-3010

issue

13

eissn

0305-1048

issn

1362-4962

journal_volume

8

pub_type

杂志文章

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