Abstract：
:In vitro selection of RNA-cleaving DNAzymes is a powerful method for isolating metal-specific DNA. A few successful examples are known, but it is still difficult to target some thiophilic metals such as Cd(2+) due to limited functional groups in DNA. While using modified bases expands the chemical functionality of DNA, a single phosphorothioate modification might boost its affinity for thiophilic metals without complicating the selection process or using bases that are not commercially available. In this work, the first such in vitro selection for Cd(2+) is reported. After using a blocking DNA and negative selections to rationally direct the library outcome, a highly specific DNAzyme with only 12 nucleotides in the catalytic loop is isolated. This DNAzyme has a cleavage rate of 0.12 min(-1) with 10 μM Cd(2+) at pH 6.0. The Rp form of the substrate is cleaved ∼100-fold faster than the Sp form. The DNAzyme is most active with Cd(2+) and its selectivity against Zn(2+) is over 100 000-fold. Its application in detecting Cd(2+) is also demonstrated. The idea of introducing single modifications in the fixed region expands the scope of DNA/metal interactions with minimal perturbation of DNA structure and property.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Huang PJ,Liu Jdoi
10.1093/nar/gkv519subject
Has Abstractpub_date
2015-07-13 00:00:00pages
6125-33issue
12eissn
0305-1048issn
1362-4962pii
gkv519journal_volume
43pub_type
杂志文章abstract：:Mitome is a specialized mitochondrial genome database designed for easy comparative analysis of various features of metazoan mitochondrial genomes such as base frequency, A+T skew, codon usage and gene arrangement pattern. A particular function of the database is the automatic reconstruction of phylogenetic relationsh...
journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2011-08-01 00:00:00
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2008-04-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.5.1265
更新日期：1993-03-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.18.3765
更新日期：1994-09-11 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/11.1.11
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更新日期：2004-04-19 00:00:00
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journal_title：Nucleic acids research
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