Abstract：
:The tumor suppressor p53 is a sequence-specific transcription factor, which regulates the expression of target genes involved in different stress responses. To understand p53's essential transcriptional functions, unbiased analysis of its DNA-binding repertoire is pivotal. In a genome-wide tiling ChIP-on-chip approach, we have identified and characterized 1546 binding sites of p53 upon Actinomycin D treatment. Among those binding sites were known as well as novel p53 target sites, which included regulatory regions of potentially novel transcripts. Using this collection of genome-wide binding sites, a new high-confidence algorithm was developed, p53scan, to identify the p53 consensus-binding motif. Strikingly, this motif was present in the majority of all bound sequences with 83% of all binding sites containing the motif. In the surrounding sequences of the binding sites, several motifs for potential regulatory cobinders were identified. Finally, we show that the majority of the genome-wide p53 target sites can also be bound by overexpressed p63 and p73 in vivo, suggesting that they can possibly play an important role at p53 binding sites. This emphasizes the possible interplay of p53 and its family members in the context of target gene binding. Our study greatly expands the known, experimentally validated p53 binding site repertoire and serves as a valuable knowledgebase for future research.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Smeenk L,van Heeringen SJ,Koeppel M,van Driel MA,Bartels SJ,Akkers RC,Denissov S,Stunnenberg HG,Lohrum Mdoi
10.1093/nar/gkn232subject
Has Abstractpub_date
2008-06-01 00:00:00pages
3639-54issue
11eissn
0305-1048issn
1362-4962pii
gkn232journal_volume
36pub_type
杂志文章abstract：:By introduction of recombinant plasmids into monkey CV1 cells, we have unambiguously demonstrated that sequences entirely within the 72 bp repeat, which is located upstream of the SV40 early region, are crucial for T-antigen expression in vivo. We have also shown that a DNA fragment containing the 72 bp repeat, insert...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.22.6047
更新日期：1981-11-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh601
更新日期：2004-05-25 00:00:00
abstract：:The crystal structure is reported of a complex between an ethyl derivative of the minor-groove drug furamidine and the dodecanucleotide duplex d(CGCGAATTCGCG)2, which has been refined to 1.85 A resolution and an R factor of 16.6% for data collected at -173 degreesC. An exceptionally large number (220) of water molecul...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.12.2873
更新日期：1998-06-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw146
更新日期：2016-07-08 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq464
更新日期：2010-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp490
更新日期：2009-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku570
更新日期：2014-07-01 00:00:00
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更新日期：2013-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2015-02-27 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2017-09-19 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2015-09-18 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1985-01-11 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/9.1.19
更新日期：1981-01-10 00:00:00
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更新日期：1991-06-25 00:00:00
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更新日期：2017-07-07 00:00:00
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更新日期：1975-08-01 00:00:00
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更新日期：2004-07-01 00:00:00
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更新日期：2003-07-01 00:00:00
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2004-07-27 00:00:00
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更新日期：1993-08-25 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/12.15.6307
更新日期：1984-08-10 00:00:00
