Abstract:
:Naturally occurring RNAs are known to exhibit a high degree of modularity, whereby specific structural modules (or motifs) can be mixed and matched to create new molecular architectures. The modular nature of RNA also affords researchers the ability to characterize individual structural elements in controlled synthetic contexts in order to gain new and critical insights into their particular structural features and overall performance. Here, we characterized the binding affinity of a unique loop-receptor interaction found in the tetrahydrofolate (THF) riboswitch using rationally designed self-assembling tectoRNAs. Our work suggests that the THF loop-receptor interaction has been fine-tuned for its particular role as a riboswitch component. We also demonstrate that the thermodynamic stability of this interaction can be modulated by the presence of folinic acid, which induces a local structural change at the level of the loop-receptor. This corroborates the existence of a THF binding site within this tertiary module and paves the way for its potential use as a THF responsive module for RNA nanotechnology and synthetic biology.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Mitchell C,Polanco JA,DeWald L,Kress D,Jaeger L,Grabow WWdoi
10.1093/nar/gkz304subject
Has Abstractpub_date
2019-07-09 00:00:00pages
6439-6451issue
12eissn
0305-1048issn
1362-4962pii
5482502journal_volume
47pub_type
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