Abstract:
:A novel class of RNA-binding proteins, Puf, regulates translation and RNA stability by binding to specific sequences in the 3'-untranslated region of target mRNAs. Members of this protein family share a conserved Puf domain consisting of eight 36 amino acid imperfect repeats. Here we report two Puf family member genes, PfPuf1 and PfPuf2, from the human malaria parasite Plasmodium falciparum. Both genes are spliced with four and three introns clustered within or near the Puf domains, respectively. Northern and RT-PCR analysis indicated that both genes were differentially expressed in gametocytes during erythrocytic development of the parasite. Except for similarities in the Puf domain and expression profile, the deduced PfPuf1 and PfPuf2 proteins differ considerably in size and structure. PfPuf1 has 1894 amino acids and a central Puf domain, whereas PfPuf2 is much smaller with a C-terminal Puf domain. The presence of at least two Puf members in other Plasmodium species suggests that these proteins play evolutionarily similar roles during parasite development. Both in vivo studies using the yeast three-hybrid system and in vitro binding assays using the recombinant Puf domain of PfPuf1 expressed in bacteria demonstrated intrinsic binding activity of the PfPuf1 Puf domain to the NRE sequences in the hunchback RNA, the target sequence for Drosophila Pumilio protein. Altogether, these results suggest that PfPufs might function during sexual differentiation and development in Plasmodium through a conserved mechanism of translational regulation of their target mRNAs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cui L,Fan Q,Li Jdoi
10.1093/nar/gkf600keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
4607-17issue
21eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract::DNA dodecamers have been designed with two cytosines on each end and intervening A and T stretches, such that the oligomers have fully complementary A:T base pairs when aligned in the parallel orientation. Spectroscopic (UV, CD and IR), NMR and molecular dynamics studies have shown that oligomers having the sequences ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.7.1500
更新日期:2002-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2017-11-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.6.1395
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:2018-11-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.16.3325
更新日期:1999-08-15 00:00:00
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pub_type: 杂志文章
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更新日期:1988-10-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2002-10-01 00:00:00
abstract::Three Z-DNA-binding proteins of Mr 31, 33 and 58 kD were isolated from mature bull testis. They were obtained in a native state suitable for binding studies. These are the first examples of Z-DNA-binding proteins from a mammalian tissue. Purification involved tissue extraction with 0.35 M NaCl, cation exchange chromat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.23.9691
更新日期:1987-12-10 00:00:00
abstract::One-Block CYRCA is an automated procedure for identifying multiple-block alignments from single block queries (http://bioinfo.weizmann.ac.il/blocks/OneCYRCA). It is based on the LAMA and CYRCA block-to-block alignment methods. The procedure identifies whether the query blocks can form new multiple-block alignments (bl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki488
更新日期:2005-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1164
更新日期:2007-01-01 00:00:00
abstract::Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.4.1059
更新日期:1983-02-25 00:00:00
abstract::E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The proce...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.13.6127
更新日期:1988-07-11 00:00:00
abstract::The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1316
更新日期:2011-04-01 00:00:00
abstract::ProPortal (http://proportal.mit.edu/) is a database containing genomic, metagenomic, transcriptomic and field data for the marine cyanobacterium Prochlorococcus. Our goal is to provide a source of cross-referenced data across multiple scales of biological organization--from the genome to the ecosystem--embracing the f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1022
更新日期:2012-01-01 00:00:00
abstract::The MED1/TRAP220 subunit of the Mediator plays a key role in facilitating ligand-dependent interactions of this multisubunit coactivator complex with nuclear receptors through their ligand binding domains. The isolated MED1/TRAP220 protein previously was shown to interact with glucocorticoid receptor (GR) in a ligand-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm661
更新日期:2007-01-01 00:00:00
abstract::Non-homologous DNA end joining (NHEJ) is considered the major pathway of double-strand break (DSB) repair in mammalian cells and depends, among other things, on the DNA end-binding Ku70/80 hetero-dimer. To investigate the function of Ku in NHEJ we have compared the ability of cell-free extracts from wild-type CHO-K1 c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.13.2585
更新日期:2000-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1117
更新日期:2016-01-04 00:00:00
abstract::Small disulfide-bonded proteins (SDPs) are rich sources for therapeutic drugs. Designing drugs from these proteins requires three-dimensional structural information, which is only available for a subset of these proteins. SDPMOD addresses this deficit in structural information by providing a freely available automated...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh394
更新日期:2004-07-01 00:00:00
abstract::The HAMSTeRS WWW site was set up in 1996 in order to facilitate easy access to, and aid understanding of, the causes of haemophilia A at the molecular level; previously, the first and second text editions of the database have been published in Nucleic Acids Research. This report describes the facilities originally ava...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.128
更新日期:1997-01-01 00:00:00
abstract::A search of sequence information in the GenBank files shows that tracts of 15-30 contiguous purines are greatly overrepresented in all eukaryotic species examined, ranging from yeast to human. Such an overabundance does not occur in prokaryotic sequences. The large increase in the number of oligopurine tracts cannot b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.4.689
更新日期:1995-02-25 00:00:00
abstract::Light is a major external factor in regulating seed germination. Photoreceptor phytochrome B (PHYB) plays a predominant role in promoting seed germination in the initial phase after imbibition, partially by repressing phytochrome-interacting factor1 (PIF1). However, the mechanism underlying the PHYB-PIF1-mediated tran...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx283
更新日期:2017-07-07 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.4.1883
更新日期:1986-02-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.6.1243
更新日期:1991-03-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.5.995
更新日期:1997-03-01 00:00:00
abstract::We have studied the structure of recombinant rat UBF (rrUBF), an RNA polymerase I transcription factor, by electron microscopy and image analysis of single particles contrasted with methylamine tungstate. Recombinant rat UBF appeared to be a flat, U-shaped protein with a central region of low density. In the dominant ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.8.1472
更新日期:1996-04-15 00:00:00
abstract::This article describes recent developments of Europe PMC (http://europepmc.org), the leading database for life science literature. Formerly known as UKPMC, the service was rebranded in November 2012 as Europe PMC to reflect the scope of the funding agencies that support it. Several new developments have enriched Europ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1061
更新日期:2015-01-01 00:00:00
abstract::The Integrated Microbial Genomes (IMG) system serves as a community resource for comparative analysis of publicly available genomes in a comprehensive integrated context. IMG integrates publicly available draft and complete genomes from all three domains of life with a large number of plasmids and viruses. IMG provide...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1044
更新日期:2012-01-01 00:00:00