Physical map and set of overlapping cosmid clones representing the genome of the archaeon Halobacterium sp. GRB.

abstract：:Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets ...

journal_title：Nucleic acids research

authors： Fedorova I,Arseniev A,Selkova P,Pobegalov G,Goryanin I,Vasileva A,Musharova O,Abramova M,Kazalov M,Zyubko T,Artamonova T,Artamonova D,Shmakov S,Khodorkovskii M,Severinov K

更新日期：2020-02-28 00:00:00

abstract：:A theoretical approach for linkage mapping the genome of any higher eukaryote is described. It uses the polymerase chain reaction, oligonucleotides of random sequence and single haploid cells. Markers are defined and then the DNA of a single sperm is broken at random (eg by gamma-rays) and physically split into 3 aliq...

journal_title：Nucleic acids research

authors： Dear PH,Cook PR

更新日期：1989-09-12 00:00:00

abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...

journal_title：Nucleic acids research

authors： Ohta S,Maruyama S,Nitta K,Sugai S

更新日期：1983-05-25 00:00:00

abstract：:A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents...

journal_title：Nucleic acids research

authors： Patel TP,Millican TA,Bose CC,Titmas RC,Mock GA,Eaton MA

更新日期：1982-09-25 00:00:00

abstract：:Cellular RNA labeling strategies based on bioorthogonal chemical reactions are much less developed in comparison to glycan, protein and DNA due to its inherent instability and lack of effective methods to introduce bioorthogonal reactive functionalities (e.g. azide) into RNA. Here we report the development of a simple...

journal_title：Nucleic acids research

authors： Sawant AA,Tanpure AA,Mukherjee PP,Athavale S,Kelkar A,Galande S,Srivatsan SG

更新日期：2016-01-29 00:00:00

abstract：:In this paper, we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy and electrophoresis methods, on three oligonucleotide sequences, each containing one 3'-3' and two 5'-5' inversion of polarity sites, and four G-runs with a variable number of residues, namely two, three and f...

journal_title：Nucleic acids research

authors： Virgilio A,Russo A,Amato T,Russo G,Mayol L,Esposito V,Galeone A

更新日期：2017-08-21 00:00:00

abstract：:The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...

journal_title：Nucleic acids research

authors： Iioka H,Loiselle D,Haystead TA,Macara IG

更新日期：2011-04-01 00:00:00

abstract：:NRPS-PKS is web-based software for analysing large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin. NRPS-PKS has been developed based on a comprehensive analysis of the sequence and structur...

journal_title：Nucleic acids research

authors： Ansari MZ,Yadav G,Gokhale RS,Mohanty D

更新日期：2004-07-01 00:00:00

abstract：:Telomeres play a central role in human cancer, cardiovascular aging and possibly longevity. However, present methods to measure telomere length are fraught with shortcomings that limit their use. Here, we describe a novel method to measure the relative telomere DNA content by dot blot analysis. In each dot, the DNA co...

journal_title：Nucleic acids research

authors： Kimura M,Aviv A

更新日期：2011-07-01 00:00:00

abstract：:Chinese hamster genomic DNA sequences from the region downstream of the dihydrofolate reductase (DHFR) gene reported to contain a chromosomal origin of bidirectional DNA replication (OBR-1) were tested for their ability to support autonomous DNA replication in human cells. A 13.3 kilobase fragment containing OBR-1 and...

journal_title：Nucleic acids research

authors： Caddle MS,Calos MP

更新日期：1992-11-25 00:00:00

abstract：:Negative feedback is known to enable biological and man-made systems to perform reliably in the face of uncertainties and disturbances. To date, synthetic biological feedback circuits have primarily relied upon protein-based, transcriptional regulation to control circuit output. Small RNAs (sRNAs) are non-coding RNA m...

journal_title：Nucleic acids research

authors： Kelly CL,Harris AWK,Steel H,Hancock EJ,Heap JT,Papachristodoulou A

更新日期：2018-10-12 00:00:00

abstract：:Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...

journal_title：Nucleic acids research

authors： Mlambo T,Nitsch S,Hildenbeutel M,Romito M,Müller M,Bossen C,Diederichs S,Cornu TI,Cathomen T,Mussolino C

更新日期：2018-05-18 00:00:00

abstract：:A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5'-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5'-end of DNA. This linkage proved to be comp...

journal_title：Nucleic acids research

authors： Fang S,Bergstrom DE

更新日期：2003-01-15 00:00:00

abstract：:Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...

journal_title：Nucleic acids research

authors： Bramsen JB,Pakula MM,Hansen TB,Bus C,Langkjær N,Odadzic D,Smicius R,Wengel SL,Chattopadhyaya J,Engels JW,Herdewijn P,Wengel J,Kjems J

更新日期：2010-09-01 00:00:00

abstract：:Herein, we describe a case study into the population dynamics of in vitro selection, using RNA-cleaving DNAzymes as a model system. We sought to understand how the composition of the population can change over time in response to different levels of selection pressure, and how well these changes are correlated with se...

journal_title：Nucleic acids research

authors： Schlosser K,Lam JC,Li Y

更新日期：2009-06-01 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:A small family of DNA sequences is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which consti...

journal_title：Nucleic acids research

authors： Allitto BA,Karrer KM

更新日期：1986-10-24 00:00:00

abstract：:Apolipoprotein B (apoB) mRNA editing is a nuclear event that minimally requires the RNA substrate, APOBEC-1 and APOBEC-1 Complementation Factor (ACF). The co-localization of these macro-molecules within the nucleus and the modulation of hepatic apoB mRNA editing activity have been described following a variety of meta...

journal_title：Nucleic acids research

authors： Lehmann DM,Galloway CA,Sowden MP,Smith HC

更新日期：2006-07-04 00:00:00

abstract：:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...

journal_title：Nucleic acids research

authors： de Felippes FF,Ott F,Weigel D

更新日期：2011-04-01 00:00:00

abstract：:The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises 42,574 serine, threoni...

journal_title：Nucleic acids research

authors： Dinkel H,Chica C,Via A,Gould CM,Jensen LJ,Gibson TJ,Diella F

更新日期：2011-01-01 00:00:00

abstract：:Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation of double-strand breaks (DSB) and, thus, to deleterious events. The first repair event occurring at a multiply damaged site (MDS) is of major importance and will largely contribute to the hazardousness of MD...

journal_title：Nucleic acids research

authors： Eot-Houllier G,Gonera M,Gasparutto D,Giustranti C,Sage E

更新日期：2007-01-01 00:00:00

abstract：:Gene expression circuitries, which enable cells to detect precise levels within a morphogen concentration gradient, have a pivotal impact on biological processes such as embryonic pattern formation, paracrine and autocrine signalling, and cellular migration. We present the rational synthesis of a synthetic genetic cir...

journal_title：Nucleic acids research

authors： Greber D,Fussenegger M

更新日期：2010-10-01 00:00:00

abstract：:Unrepaired DNA damage may arrest ongoing replication forks, potentially resulting in fork collapse, increased mutagenesis and genomic instability. Replication through DNA lesions depends on mono- and polyubiquitylation of proliferating cell nuclear antigen (PCNA), which enable translesion synthesis (TLS) and template ...

journal_title：Nucleic acids research

authors： Juhasz S,Balogh D,Hajdu I,Burkovics P,Villamil MA,Zhuang Z,Haracska L

更新日期：2012-11-01 00:00:00

abstract：:Thiol-activated neocarzinostatin chromophore abstracts tritium from the 5', but not from the 1' or 2' positions of deoxyribose in DNA and incorporates it into a stable, non-exchangeable form. The abstracted tritium remains covalently associated with the chromophore or its degradation product after treatment with acid ...

journal_title：Nucleic acids research

authors： Kappen LS,Goldberg IH

更新日期：1985-03-11 00:00:00

abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...

journal_title：Nucleic acids research

authors： Mitxelena J,Apraiz A,Vallejo-Rodríguez J,Malumbres M,Zubiaga AM

更新日期：2016-07-08 00:00:00

abstract：:Using sedimentation and cryo electron tomography techniques, the conformations of eukaryotic polyribosomes formed in a long-term cell-free translation system were analyzed over all the active system lifetime (20-30 translation rounds during 6-8 h in wheat germ extract at 25°C). Three distinct types of the conformation...

journal_title：Nucleic acids research

authors： Afonina ZA,Myasnikov AG,Shirokov VA,Klaholz BP,Spirin AS

更新日期：2015-01-01 00:00:00

abstract：:Negative superhelical strain induces the poly(dG)-poly(dC) sequence to adopt two totally different types of triple-helices, either a dG.dG.dC triplex in the presence of Mg(+)+ at both neutral and acidic pHs or a protonated dC+.dG.dC triplex in the absence of Mg(+)+ ions at acidic pH (1). To examine whether there are s...

journal_title：Nucleic acids research

authors： Kohwi-Shigematsu T,Kohwi Y

更新日期：1991-08-11 00:00:00

abstract：:RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...

journal_title：Nucleic acids research

authors： Berzin V,Cielens I,Jansone I,Gren EJ

更新日期：1982-12-11 00:00:00

abstract：:Bacterial RNase III plays important roles in the processing and degradation of RNA transcripts. A major goal is to identify the cleavage targets of this endoribonuclease at a transcriptome-wide scale and delineate its in vivo cleavage rules. Here we applied to Escherichia coli grown to either exponential or stationary...

journal_title：Nucleic acids research

authors： Altuvia Y,Bar A,Reiss N,Karavani E,Argaman L,Margalit H

更新日期：2018-11-02 00:00:00

abstract：:Synchronous plasmodia of cycloheximide-sensitive and cycloheximide-resistant strains of Physarum polycephalum were labelled with 3[H]-deoxyadenosine in pulse and pulse-chase experiments in presence and absence of cycloheximide. The replication products were studied with alkaline sucrose gradient sedimentation analysis...

journal_title：Nucleic acids research

authors： Funderud S,Haugli F

更新日期：1977-02-01 00:00:00