Abstract:
:A theoretical approach for linkage mapping the genome of any higher eukaryote is described. It uses the polymerase chain reaction, oligonucleotides of random sequence and single haploid cells. Markers are defined and then the DNA of a single sperm is broken at random (eg by gamma-rays) and physically split into 3 aliquots. Each aliquot is screened for the presence of each marker. Closely-linked markers are more likely to be found in the same aliquot than unlinked markers. The entire process is repeated with further sperm and the frequency that any two markers co-segregate determined. Closely-linked markers co-segregate from most cells; unlinked markers do so rarely. A map can then be constructed from these co-segregation frequencies. A specific application for determining the order and distance between sets of closely-linked and previously-defined markers is also described.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dear PH,Cook PRdoi
10.1093/nar/17.17.6795subject
Has Abstractpub_date
1989-09-12 00:00:00pages
6795-807issue
17eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract::Many genetic manipulations are limited by difficulty in obtaining adequate levels of protein expression. Bioinformatic and experimental studies have identified nucleotide sequence features that may increase expression, however it is difficult to assess the relative influence of these features. Zebrafish embryos are ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv035
更新日期:2015-04-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv959
更新日期:2015-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1195
更新日期:2021-01-11 00:00:00
abstract::In an extensive analysis, using a range of restriction endonucleases, HinfI and TaqI were found to differentiate satellites I, II and III & IV. Satellite I is resistant to digestion by TaqI, but is cleaved by HinfI to yield three major fragments of approximate size 770, 850 and 950bp, associated in a single length of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.2.547
更新日期:1982-01-22 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1146
更新日期:2018-01-25 00:00:00
abstract::About 40% of the genes in the nematode Caenorhabditis elegans have homologs in humans. Based on the history of this model system, it is clear that the application of genetic methods to the study of this set of genes would provide important clues to their function in humans. To facilitate such genetic studies, we are e...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnf051
更新日期:2002-06-15 00:00:00
abstract::With the rapid accumulation of our knowledge on diseases, disease-related genes and drug targets, network-based analysis plays an increasingly important role in systems biology, systems pharmacology and translational science. The new release of VisANT aims to provide new functions to facilitate the convenient network ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt401
更新日期:2013-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.4.1059
更新日期:1983-02-25 00:00:00
abstract::A DNA fragment d(GCGAAAGCT), known to adopt a stable mini-hairpin structure in solution, has been crystallized in the space group I4(1)22 with the unit-cell dimensions a = b = 53.4 A and c = 54.0 A, and the crystal structure has been determined at 2.5 A resolution. The four nucleotide residues CGAA of the first half o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf639
更新日期:2002-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkz1101
更新日期:2020-01-10 00:00:00
abstract::As an increasing number of reliable protein-protein interactions (PPIs) become available and high-throughput experimental methods provide systematic identification of PPIs, there is a growing need for fast and accurate methods for discovering homologous PPIs of a newly determined PPI. PPISearch is a web server that ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp309
更新日期:2009-07-01 00:00:00
abstract::The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq318
更新日期:2010-07-01 00:00:00
abstract::In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by Dahm and Uhlenbeck ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.24.5656
更新日期:1993-12-11 00:00:00
abstract::We present the sequences of five additional human U2 pseudogenes which are very similar to the U2.13 pseudogene reported previously [Van Arsdell et al. (1981) Cell 26, 11-17]. All six U2 pseudogenes preserve the 5' end of the mature U2 snRNA sequence, and all six are flanked by nearly perfect direct repeats that diffe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.3.1463
更新日期:1984-02-10 00:00:00
abstract::Gene finding is complicated in organisms that exhibit insertional RNA editing. Here, we demonstrate how our new algorithm Predictor of Insertional Editing (PIE) can be used to locate genes whose mRNAs are subjected to multiple frameshifting events, and extend the algorithm to include probabilistic predictions for site...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki820
更新日期:2005-09-07 00:00:00
abstract::Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.8.1857
更新日期:1976-08-01 00:00:00
abstract::Explicit solvent molecular dynamics (MD) was used to describe the intrinsic flexibility of the helix 42-44 portion of the 23S rRNA (abbreviated as Kt-42+rGAC; kink-turn 42 and GTPase-associated center rRNA). The bottom part of this molecule consists of alternating rigid and flexible segments. The first flexible segmen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm245
更新日期:2007-01-01 00:00:00
abstract::The combination of RNA interference (RNAi) with the tetracycline-controlled transcription activation (tet) system promises to become a powerful method for conditional gene inactivation in cultured cells and in whole organisms. Here, we tested critical sequence elements that originated from miRNA mR-30 for optimal effi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq616
更新日期:2010-09-01 00:00:00
abstract::The eukaryotic ribosomal protein S26e (rpS26e) lacking eubacterial counterparts is a key component of the ribosomal binding site of mRNA region 5' of the codon positioned at the exit site. Here, we determined the rpS26e oligopeptide neighboring mRNA on the human 80S ribosome using mRNA analogues bearing perfluoropheny...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks372
更新日期:2012-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-04-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2017-03-17 00:00:00
abstract::Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.12.2907
更新日期:1993-06-25 00:00:00
abstract::Non-coding RNA regulatory elements are important for viral replication, making them promising targets for therapeutic intervention. However, regulatory RNA is challenging to detect and characterise using classical structure-function assays. Here, we present in cell Mutational Interference Mapping Experiment (in cell M...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky152
更新日期:2018-05-18 00:00:00
abstract::In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.19.5787
更新日期:1990-10-11 00:00:00
abstract::Arginyl-tRNA synthetase (ArgRS) catalyses AMP- and PPi-independent deacylation of Arg-tRNAArg in the presence of cysteine. A dipeptide, Arg-Cys, is a product of this deacylation reaction. Similar reaction with homocysteine yields Arg-Hcy. Arginine is a noncompetitive inhibitor of the cysteine-dependent deacylation whi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.22.4608
更新日期:1995-11-25 00:00:00
abstract::The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a vi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1070
更新日期:2011-02-01 00:00:00
abstract::Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images a...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1993-12-25 00:00:00
abstract::The vitamin D receptor mediates the signal of 1 alpha, 25-dihydroxyvitamin D3 by binding to vitamin D responsive elements in DNA as a homodimer or as a heterodimer composed of one vitamin D receptor subunit and one retinoid X receptor subunit. We have mapped the dimerization interfaces of the vitamin D receptor that i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.4.606
更新日期:1995-02-25 00:00:00
abstract::We previously determined that a linear co-polymer of histidine and lysine (HK) in combination with liposomes enhanced the transfection efficiency of cationic liposomes. In the current study, we designed a series of HK polymers with increased branching and/or histidine/lysine ratio to determine if either variable affec...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.6.1334
更新日期:2001-03-15 00:00:00