Abstract:
:In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show that neither cell viability nor growth rate is affected by the T7-driven transcription of these genes, provided that they are present as single chromosomal copy. Furthermore, transcription is now completely uncoupled from translation, allowing large amounts of even completely untranslated mRNAs to be synthesized. Taking advantage of these features, we discuss the influence of the frequency of translation upon the processing and degradation of the lac message.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chevrier-Miller M,Jacques N,Raibaud O,Dreyfus Mdoi
10.1093/nar/18.19.5787subject
Has Abstractpub_date
1990-10-11 00:00:00pages
5787-92issue
19eissn
0305-1048issn
1362-4962journal_volume
18pub_type
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