DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system.

abstract：:It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-le...

journal_title：Nucleic acids research

authors： Kataoka N,Ohno M,Moda I,Shimura Y

更新日期：1995-09-25 00:00:00

abstract：:The design and synthesis of metal complexes that can specifically target DNA secondary structure has attracted considerable attention. Chiral metallosupramolecular complexes (e.g. helicates) in particular display unique DNA-binding behavior, however until recently few examples which are both water-compatible and enant...

journal_title：Nucleic acids research

authors： Zhao A,Howson SE,Zhao C,Ren J,Scott P,Wang C,Qu X

更新日期：2017-05-19 00:00:00

abstract：:We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly purified 5-MeC-DNA glycosylase complex were identified by Nanoelectrospray ionisation mass spectrometry to be identical to the...

journal_title：Nucleic acids research

authors： Jost JP,Schwarz S,Hess D,Angliker H,Fuller-Pace FV,Stahl H,Thiry S,Siegmann M

更新日期：1999-08-15 00:00:00

abstract：:Significant differences are observed between the CD spectra of eu- and heterochromatin prepared by fractionating mechanically sheared rat liver chromatin on linear sucrose density gradients. Heterochromatin has a broad positive CD at 270--280 nm, a cross-over point at 256 nmn, and a negative shoulder at 248 nm. Euchro...

journal_title：Nucleic acids research

authors： Moyer GH,Kay E,Austin GE

更新日期：1979-04-01 00:00:00

abstract：:The products of the RAG-1 and RAG-2 genes are essential for the recombination of the DNA encoding the antigen receptors of the developing immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing loca...

journal_title：Nucleic acids research

authors： Sadofsky MJ,Hesse JE,McBlane JF,Gellert M

更新日期：1993-12-11 00:00:00

abstract：:BRCA1 expression is repressed by aberrant cytosine methylation in sporadic breast cancer. We hypothesized that aberrant cytosine methylation of the BRCA1 promoter was associated with the transcriptionally repressive effects of histone hypoacetylation and chromatin condensation. To address this question, we developed a...

journal_title：Nucleic acids research

authors： Rice JC,Futscher BW

更新日期：2000-09-01 00:00:00

abstract：:Cytokines are cell-to-cell signaling proteins that play a central role in immune development, pathogen responses, and diseases. Cytokines are highly regulated at the transcriptional level by combinations of transcription factors (TFs) that recruit cofactors and the transcriptional machinery. Here, we mined through thr...

journal_title：Nucleic acids research

authors： Carrasco Pro S,Dafonte Imedio A,Santoso CS,Gan KA,Sewell JA,Martinez M,Sereda R,Mehta S,Fuxman Bass JI

更新日期：2018-10-12 00:00:00

abstract：:The stable intermediates formed in the reaction of cytosine, cytidine and adenosine with chloracetaldehyde were isolated. The -CH2CH/OH/- bridge between the exo and endo nitrogen atoms of the parent base was found in these compounds by means of PMR spectroscopy. Their acid-induced dehydration resulted in formation of ...

journal_title：Nucleic acids research

authors： Biernat J,Ciesiołka J,Górnicki P,Adamiak RW,Kryzosiak WJ,Wiewiórowski M

更新日期：1978-03-01 00:00:00

abstract：:It is a central issue to elucidate the new type of molecular recognition accompanied by a global structural change of a molecule upon binding to its targets. Here we investigate the driving force for the binding of R12 (a ribonucleic acid aptamer) and P16 (a partial peptide of a prion protein) during which P16 exhibit...

journal_title：Nucleic acids research

authors： Hayashi T,Oshima H,Mashima T,Nagata T,Katahira M,Kinoshita M

更新日期：2014-06-01 00:00:00

abstract：:UMSBP is a CCHC-type zinc finger protein, which functions during replication initiation of kinetoplast DNA minicircles and the segregation of kinetoplast DNA networks. Interactions of UMSBP with origin sequences, as well as the protein oligomerization, are affected by its redox state. Reduction yields UMSBP monomers a...

journal_title：Nucleic acids research

authors： Sela D,Shlomai J

更新日期：2009-01-01 00:00:00

abstract：:Mammalian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation. Phosphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication. Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. The ...

journal_title：Nucleic acids research

authors： Estève PO,Zhang G,Ponnaluri VK,Deepti K,Chin HG,Dai N,Sagum C,Black K,Corrêa IR Jr,Bedford MT,Cheng X,Pradhan S

更新日期：2016-02-29 00:00:00

abstract：:Gene expression patterns have been demonstrated to be highly variable between similar cell types, for example lab strains and wild strains of Saccharomyces cerevisiae cultured under identical growth conditions exhibit a wide range of expression differences. We have used a genome-wide approach to characterize transcrip...

journal_title：Nucleic acids research

authors： Llinás M,Bozdech Z,Wong ED,Adai AT,DeRisi JL

更新日期：2006-02-21 00:00:00

abstract：:Adenosine-to-inosine (A-to-I) RNA editing is a common post transcriptional modification. It has a critical role in protecting against false activation of innate immunity by endogenous double stranded RNAs and has been associated with various regulatory processes and diseases such as autoimmune and cardiovascular disea...

journal_title：Nucleic acids research

authors： Schaffer AA,Kopel E,Hendel A,Picardi E,Levanon EY,Eisenberg E

更新日期：2020-06-19 00:00:00

abstract：:A simple, two-step method is described for the synthesis of the 5'-pyro- and triphosphate derivatives of 3'-5' ApA, ApG, GpA and GpG. The readily accessible 2'(3')-5' ApA, ApG, GpA and GpG were converted in one step to the corresponding 5'-phosphoramidate derivatives which were then transformed to the 5'-pyro- and tri...

journal_title：Nucleic acids research

authors： Tomasz J,Simoncsits A,Kajtár M,Krug RM,Shatkin AJ

更新日期：1978-08-01 00:00:00

abstract：:A non-redundant database of nuclear, protein-encoding, genomic DNA sequences highlighting nuclear pre-mRNA introns was constructed using information contained in the SWISS-PROT and GenBank sequence databases. This Intron DataBase (IDB) contains information about (i) introns (including nucleotide sequence, location, ph...

journal_title：Nucleic acids research

authors： Schisler NJ,Palmer JD

更新日期：2000-01-01 00:00:00

abstract：:The BRENDA enzyme information system (http://www.brenda-enzymes.org/) has developed into an elaborate system of enzyme and enzyme-ligand information obtained from different sources, combined with flexible query systems and evaluation tools. The information is obtained by manual extraction from primary literature, text...

journal_title：Nucleic acids research

authors： Chang A,Schomburg I,Placzek S,Jeske L,Ulbrich M,Xiao M,Sensen CW,Schomburg D

更新日期：2015-01-01 00:00:00

abstract：:RNA synthesis and DNA replication cease after DNA damage. We studied RNA synthesis using an in situ run-on assay and found ribosomal RNA (rRNA) synthesis was inhibited 24 h after UV light, gamma radiation or DNA cross-linking by cisplatin in human cells. Cisplatin led to accumulation of cells in S phase. Inhibition of...

journal_title：Nucleic acids research

authors： Calkins AS,Iglehart JD,Lazaro JB

更新日期：2013-08-01 00:00:00

abstract：:DNA double stranded breaks (DSBs) are one of the most deleterious types of DNA lesions. The main pathways responsible for repairing these breaks in eukaryotic cells are homologous recombination (HR) and non-homologous end-joining (NHEJ). However, a third group of still poorly characterized DSB repair pathways, collect...

journal_title：Nucleic acids research

authors： Kostyrko K,Mermod N

更新日期：2016-04-07 00:00:00

abstract：:A minor tRNA-Glu1 constituent of a preparation of E. coli B tRNA-Glu (Oak Ridge) has a guanosine residue at position 66 rather than an adenosine as in the tRNA-Glu2 described by Ohashi et al. (3). Automated sequential degradation was used to sequence this region. ...

journal_title：Nucleic acids research

authors： Uziel M,Weinberger AJ

更新日期：1975-04-01 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:Genome browsers are widely used for individually exploring various types of genomic data. A handful of genome browsers offer limited tools for collaboration among multiple users. Here, we describe PBrowse, an integrated real-time collaborative genome browser that enables multiple users to simultaneously view and acces...

journal_title：Nucleic acids research

authors： Szot PS,Yang A,Wang X,Parsania C,Röhm U,Wong KH,Ho JWK

更新日期：2017-05-19 00:00:00

abstract：:As large-scale re-sequencing of genomes reveals many protein mutations, especially in human cancer tissues, prediction of their likely functional impact becomes important practical goal. Here, we introduce a new functional impact score (FIS) for amino acid residue changes using evolutionary conservation patterns. The ...

journal_title：Nucleic acids research

authors： Reva B,Antipin Y,Sander C

更新日期：2011-09-01 00:00:00

abstract：:We have examined the transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 in UV irradiated cells, in the mitotic cell cycle, and during meiosis. Levels of RAD18 mRNA increased upon UV irradiation, but remained constant during the mitotic cell cycle. During meiosis, RAD18 mRNA levels rose about 4 fol...

journal_title：Nucleic acids research

authors： Jones JS,Prakash L

更新日期：1991-02-25 00:00:00

abstract：:The European Nucleotide Archive (ENA; https://www.ebi.ac.uk/ena), provided by the European Molecular Biology Laboratory's European Bioinformatics Institute (EMBL-EBI), has for almost forty years continued in its mission to freely archive and present the world's public sequencing data for the benefit of the entire scie...

journal_title：Nucleic acids research

authors： Harrison PW,Ahamed A,Aslam R,Alako BTF,Burgin J,Buso N,Courtot M,Fan J,Gupta D,Haseeb M,Holt S,Ibrahim T,Ivanov E,Jayathilaka S,Balavenkataraman Kadhirvelu V,Kumar M,Lopez R,Kay S,Leinonen R,Liu X,O'Cathail C,Pa

更新日期：2021-01-08 00:00:00

abstract：:The NusA protein is a universally conserved bacterial transcription elongation factor that binds RNA polymerase (RNAP). When functioning independently, NusA enhances intrinsic termination. Paradoxically, NusA stimulates the function of the N and Q antiterminator proteins of bacteriophage λ. The mechanistic basis for N...

journal_title：Nucleic acids research

authors： Wells CD,Deighan P,Brigham M,Hochschild A

更新日期：2016-06-20 00:00:00

abstract：:The Homeodomain Resource is a comprehensive collection of sequence, structure and genomic information on the homeodomain protein family. Available through the Resource are both full-length and domain-only sequence data, as well as X-ray and NMR structural data for proteins and protein-DNA complexes. Also available is ...

journal_title：Nucleic acids research

authors： Banerjee-Basu S,Ferlanti ES,Ryan JF,Baxevanis AD

更新日期：1999-01-01 00:00:00

abstract：:We have created a database of comparatively derived group I intron secondary structure diagrams. This collection currently contains a broad sampling of phylogenetically and structurally similar and diverse structures from over 200 publicly available intron sequences. As more group I introns are sequenced and added to ...

journal_title：Nucleic acids research

authors： Damberger SH,Gutell RR

更新日期：1994-09-01 00:00:00

abstract：:As the formation of ribonucleoprotein complexes is a major mechanism of angiotensin II type 1 receptor (AT1R) regulation, we sought to identify novel AT1R mRNA binding proteins. By affinity purification and mass spectroscopy, we identified TIA-1. This interaction was confirmed by colocalization of AT1R mRNA and TIA-1 ...

journal_title：Nucleic acids research

authors： Backlund M,Paukku K,Kontula KK,Lehtonen JY

更新日期：2016-04-20 00:00:00

abstract：:Alignment-based programs are valuable tools for finding potential homologs in genome sequences. Previously, it has been shown that partition function posterior probabilities attuned to local alignment achieve a high accuracy in identifying distantly similar non-coding RNA sequences that are hidden in a large genome. H...

journal_title：Nucleic acids research

authors： Chikkagoudar S,Livesay DR,Roshan U

更新日期：2010-07-01 00:00:00

abstract：:Three-dimensional protein structures provide invaluable information for understanding and regulating biological functions of proteins. The GalaxyWEB server predicts protein structure from sequence by template-based modeling and refines loop or terminus regions by ab initio modeling. This web server is based on the met...

journal_title：Nucleic acids research

authors： Ko J,Park H,Heo L,Seok C

更新日期：2012-07-01 00:00:00