Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication.

abstract：:Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

journal_title：Nucleic acids research

authors： Hackney JA,Ehrenkaufer GM,Singh U

更新日期：2007-01-01 00:00:00

abstract：:Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existence of a stable cross...

journal_title：Nucleic acids research

authors： Woppmann A,Rinke J,Lührmann R

更新日期：1988-12-09 00:00:00

abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...

journal_title：Nucleic acids research

authors： Hulla JE,Schneider RP

更新日期：1993-02-11 00:00:00

abstract：:UNITE (https://unite.ut.ee/) is a web-based database and sequence management environment for the molecular identification of fungi. It targets the formal fungal barcode-the nuclear ribosomal internal transcribed spacer (ITS) region-and offers all ∼1 000 000 public fungal ITS sequences for reference. These are clustere...

journal_title：Nucleic acids research

authors： Nilsson RH,Larsson KH,Taylor AFS,Bengtsson-Palme J,Jeppesen TS,Schigel D,Kennedy P,Picard K,Glöckner FO,Tedersoo L,Saar I,Kõljalg U,Abarenkov K

更新日期：2019-01-08 00:00:00

abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...

journal_title：Nucleic acids research

authors： Kreivi JP,Akusjärvi G

更新日期：1994-02-11 00:00:00

abstract：:Heterotrimeric eukaryotic/archaeal translation initiation factor 2 (e/aIF2) binds initiator methionyl-tRNA and plays a key role in the selection of the start codon on messenger RNA. tRNA binding was extensively studied in the archaeal system. The γ subunit is able to bind tRNA, but the α subunit is required to reach h...

journal_title：Nucleic acids research

authors： Naveau M,Lazennec-Schurdevin C,Panvert M,Dubiez E,Mechulam Y,Schmitt E

更新日期：2013-01-01 00:00:00

abstract：:Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...

journal_title：Nucleic acids research

authors： St Laurent G,Vyatkin Y,Antonets D,Ri M,Qi Y,Saik O,Shtokalo D,de Hoon MJ,Kawaji H,Itoh M,Lassmann T,Arner E,Forrest AR,FANTOM consortium.,Nicolas E,McCaffrey TA,Carninci P,Hayashizaki Y,Wahlestedt C,Kapranov P

更新日期：2016-04-20 00:00:00

abstract：:Anisotropic motions of DNA fragments in the size range 6-118 base pairs are studied by the steady-state fluorescence polarization of different excitation transitions in the intercalated ethidium cation. Calculated effective tumbling and twisting times are found to be shorter than predicted for overall motions of rigid...

journal_title：Nucleic acids research

authors： Härd T,Kearns DR

更新日期：1986-05-12 00:00:00

abstract：:Ovarian poly (A) + RNA from Xenopus laevis and Xenopus borealis was used to construct two cDNA libraries which were screened for histone sequences. cDNA clones to H4 mRNA were obtained from both species and an H3 cDNA clone from Xenopus laevis. The complete DNA sequences of these clones have been determined and are pr...

journal_title：Nucleic acids research

authors： Turner PC,Woodland HR

更新日期：1982-06-25 00:00:00

abstract：:REBASE is a comprehensive database of information about restriction enzymes and their associated methylases, including their recognition and cleavage sites and their commercial availability. Also included is a listing of homing endonucleases. Information from REBASE is available via monthly electronic mailings as well...

journal_title：Nucleic acids research

authors： Roberts RJ,Macelis D

更新日期：1998-01-01 00:00:00

abstract：:The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucle...

journal_title：Nucleic acids research

authors： Fuke M,Busch H

更新日期：1977-02-01 00:00:00

abstract：:As more and more high-throughput data has been produced by next-generation sequencing, it is still a challenge to classify RNA transcripts into protein-coding or non-coding, especially for poorly annotated species. We upgraded our original coding potential calculator, CNCI (Coding-Non-Coding Index), to CNIT (Coding-No...

journal_title：Nucleic acids research

authors： Guo JC,Fang SS,Wu Y,Zhang JH,Chen Y,Liu J,Wu B,Wu JR,Li EM,Xu LY,Sun L,Zhao Y

更新日期：2019-07-02 00:00:00

abstract：:The small proline-rich protein genes ( SPRRs ) code for precursors of the cornified cell envelope, and are specifically expressed during keratinocyte terminal differentiation. The single intron of SPRR2A enhanced the activity of the SPRR2A promoter in transient transfection assays. This enhancement was position depend...

journal_title：Nucleic acids research

authors： Fischer DF,van Drunen CM,Winkler GS,van de Putte P,Backendorf C

更新日期：1998-12-01 00:00:00

abstract：:We describe snap-to-it probes, a novel probe technology to enhance the hybridization specificity of natural and unnatural nucleic acid oligomers using a simple and readily introduced structural motif. Snap-to-it probes were prepared from peptide nucleic acid (PNA) oligomers by modifying each terminus with a coordinati...

journal_title：Nucleic acids research

authors： Morgan JR,Lyon RP,Maeda DY,Zebala JA

更新日期：2008-06-01 00:00:00

abstract：:Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that D...

journal_title：Nucleic acids research

authors： Roberti M,Polosa PL,Bruni F,Musicco C,Gadaleta MN,Cantatore P

更新日期：2003-03-15 00:00:00

abstract：:In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show t...

journal_title：Nucleic acids research

authors： Chevrier-Miller M,Jacques N,Raibaud O,Dreyfus M

更新日期：1990-10-11 00:00:00

abstract：:During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We have cloned a new RNA helicase gene, designated DBP2 (DEAH-boxprotein), homologous to the Schizosaccaromyces pombe cdc28(+...

journal_title：Nucleic acids research

authors： Imamura O,Saiki K,Tani T,Ohshima Y,Sugawara M,Furuichi Y

更新日期：1998-05-01 00:00:00

abstract：:Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter PLtetO-1 allows the regulation of ge...

journal_title：Nucleic acids research

authors： Lutz R,Bujard H

更新日期：1997-03-15 00:00:00

abstract：:The variation of the proton chemical shifts due to the formation intermolecular hydrogen bonds is computed for a number of complexes which can be formed between the bases of the nucleic acids. The shifts expected for the isolated base pairs, in particular for the G-N1 H, T(or U)-N3H protons and the protons of the amin...

journal_title：Nucleic acids research

authors： Giessner-Prettre C,Pullman B,Caillet J

更新日期：1977-01-01 00:00:00

abstract：:A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing...

journal_title：Nucleic acids research

authors： Rommens J,MacKnight D,Pomeroy-Cloney L,Jay E

更新日期：1983-09-10 00:00:00

abstract：:Accumulating evidence has demonstrated that transcriptional regulation is affected by DNA methylation. Understanding the perturbation of DNA methylation-mediated regulation between transcriptional factors (TFs) and targets is crucial for human diseases. However, the global landscape of DNA methylation-mediated transcr...

journal_title：Nucleic acids research

authors： Wang Z,Yin J,Zhou W,Bai J,Xie Y,Xu K,Zheng X,Xiao J,Zhou L,Qi X,Li Y,Li X,Xu J

更新日期：2020-03-18 00:00:00

abstract：:Live-cell measurement of protein binding to chromatin allows probing cellular biochemistry in physiological conditions, which are difficult to mimic in vitro. However, different studies have yielded widely discrepant predictions, and so it remains uncertain how to make the measurements accurately. To establish a bench...

journal_title：Nucleic acids research

authors： Mazza D,Abernathy A,Golob N,Morisaki T,McNally JG

更新日期：2012-08-01 00:00:00

abstract：:The Escherichia coli AlkB protein catalyzes the direct reversal of alkylation damage to DNA; primarily 1-methyladenine (1mA) and 3-methylcytosine (3mC) lesions created by endogenous or environmental alkylating agents. AlkB is a member of the non-heme iron (II) alpha-ketoglutarate-dependent dioxygenase superfamily, whi...

journal_title：Nucleic acids research

authors： Roy TW,Bhagwat AS

更新日期：2007-01-01 00:00:00

abstract：:We have used paired-end sequencing of yeast nucleosomal DNA to obtain accurate genomic maps of nucleosome positions and occupancies in control cells and cells treated with 3-aminotriazole (3AT), an inducer of the transcriptional activator Gcn4. In control cells, 3AT-inducible genes exhibit a series of distinct nucleos...

journal_title：Nucleic acids research

authors： Cole HA,Howard BH,Clark DJ

更新日期：2011-12-01 00:00:00

abstract：:Increasing experimental evidence shows a prominent role of histone modifications in the coordinated control of gene expression in the human malaria parasite Plasmodium falciparum. The search for the histone-mark-reading machinery that translates histone modifications into biological processes, such as formation of het...

journal_title：Nucleic acids research

authors： Pérez-Toledo K,Rojas-Meza AP,Mancio-Silva L,Hernández-Cuevas NA,Delgadillo DM,Vargas M,Martínez-Calvillo S,Scherf A,Hernandez-Rivas R

更新日期：2009-05-01 00:00:00

abstract：:In eukaryotic ribosome, the N domain of polypeptide release factor eRF1 is involved in decoding stop signals in mRNAs. However, structure of the decoding site remains obscure. Here, we specifically altered the stop codon recognition pattern of human eRF1 by point mutagenesis of the invariant Glu55 and Tyr125 residues ...

journal_title：Nucleic acids research

authors： Kolosov P,Frolova L,Seit-Nebi A,Dubovaya V,Kononenko A,Oparina N,Justesen J,Efimov A,Kisselev L

更新日期：2005-11-10 00:00:00

abstract：:We present evidence of formation of an intramolecular parallel triple helix with T*A.T and G*G.C base triplets (where * represents the hydrogen bonding interaction between the third strand and the duplex while. represents the Watson-Crick interactions which stabilize the duplex). The third GT strand, containing seven ...

journal_title：Nucleic acids research

authors： Khomyakova EB,Gousset H,Liquier J,Huynh-Dinh T,Gouyette C,Takahashi M,Florentiev VL,Taillandier E

更新日期：2000-09-15 00:00:00

abstract：:Identification of common sub-sequences for a group of functionally related DNA sequences can shed light on the role of such elements in cell-specific gene expression. In the megakaryocytic lineage, no one single unique transcription factor was described as linage specific, raising the possibility that a cluster of gen...

journal_title：Nucleic acids research

authors： Hazony Y,Lu J,St Hilaire C,Ravid K

更新日期：2006-01-01 00:00:00

abstract：:RNase P RNA (RPR), the catalytic subunit of the essential RNase P ribonucleoprotein, removes the 5' leader from precursor tRNAs. The ancestral eukaryotic RPR is a Pol III transcript generated with mature termini. In the branch of the arthropod lineage that led to the insects and crustaceans, however, a new allele aros...

journal_title：Nucleic acids research

authors： Palsule G,Gopalan V,Simcox A

更新日期：2019-09-19 00:00:00

abstract：:Oligoribonucleotides containing a 5'-phosphorothiolate linkage have provided effective tools to study the mechanisms of RNA catalysis, allowing resolution of kinetic ambiguity associated with mechanistic dissection and providing a strategy to establish linkage between catalysis and specific functional groups. However,...

journal_title：Nucleic acids research

authors： Li NS,Frederiksen JK,Koo SC,Lu J,Wilson TJ,Lilley DM,Piccirilli JA

更新日期：2011-03-01 00:00:00