Abstract:
:The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J. Mol. Biol. 184: 413-428, 1985). Here we show that coI intron 1 is a typical Group II intron, the only one identified to date in Neurospora. The differential effect of the cyt18-1 mutation suggests that splicing of certain introns could be regulated independently of others by nuclear-encoded proteins. The intron contains a long open reading frame (ORF) resembling that of the Neurospora Mauriceville mitochondrial plasmid. The intron and plasmid ORFs share unusual features of codon usage that suggest both evolved outside of the Neurospora mitochondrial genetic system.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Field DJ,Sommerfield A,Saville BJ,Collins RAdoi
10.1093/nar/17.22.9087subject
Has Abstractpub_date
1989-11-25 00:00:00pages
9087-99issue
22eissn
0305-1048issn
1362-4962journal_volume
17pub_type
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