Abstract：
:The Aspergillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of argB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ans1 or by cotransforming selectable plasmids (containing argB, trpC, or pyr4) with the non-selectable ans1 sequence. The preponderance of evidence suggests that the mechanism of ans1 activity does not involve homologous recombination events, in spite of the presence of multiple regions of homology in the A. nidulans genome. Genetic mapping localized ans1 to the vicinity of the centromere of linkage group I. The nucleotide sequence of a 1.8 Kb functional subclone of ans1 was determined and found to be highly A + T rich (81%).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cullen D,Wilson LJ,Grey GL,Henner DJ,Turner G,Ballance DJdoi
10.1093/nar/15.22.9163subject
Has Abstractpub_date
1987-11-25 00:00:00pages
9163-75issue
22eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract：:Potential promoters in the genome of Escherichia coli were searched by pattern recognition software PlatProm and classified on the basis of positions relative to gene borders. Beside the expected promoters located in front of the coding sequences we found a considerable amount of intragenic promoter-like signals with ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp490
更新日期：2009-08-01 00:00:00
abstract：:Conventional chemotherapy remains the primary treatment option for triple-negative breast cancer (TNBC). However, the current chemotherapeutic drugs have limited effects on TNBC, and often lead to serious side effects as well as drug resistance. Thus, more effective therapeutic options are sorely needed. As c-MYC onco...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz835
更新日期：2019-11-18 00:00:00
abstract：:Directed molecular evolution and combinatorial methodologies are playing an increasingly important role in the field of protein engineering. The general approach of generating a library of partially randomized genes, expressing the gene library to generate the proteins the library encodes and then screening the protei...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkh315
更新日期：2004-02-27 00:00:00
abstract：:We recently reported that the antitumor triazoloacridone, compound C-1305, is a topoisomerase II poison with unusual properties. In this study we characterize the DNA interactions of C-1305 in vitro, in comparison with other topoisomerase II inhibitors. Our results show that C-1305 binds to DNA by intercalation and po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki904
更新日期：2005-10-27 00:00:00
abstract：:The thermodynamics of 13 hybridization reactions between 10 base DNA sequences of design 5'-ATGCXYATGC-3' with X, Y = A, C, G, T and their complementary PNA and DNA sequences were determined from isothermal titration calorimetry (ITC) measurements at ambient temperature. For the PNA/DNA hybridization reactions, the bi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.24.4792
更新日期：1999-12-15 00:00:00
abstract：:Bacteria adapt to changes in their environment via differential gene expression mediated by DNA binding transcriptional regulators. The PRODORIC2 database hosts one of the largest collections of DNA binding sites for prokaryotic transcription factors. It is the result of the thoroughly redesigned PRODORIC database. PR...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1091
更新日期：2018-01-04 00:00:00
abstract：:The Neurospora VS ribozyme is a catalytic RNA that has the unique ability to specifically recognize and cleave a stem-loop substrate through formation of a highly stable kissing-loop interaction (KLI). In order to explore the engineering potential of the VS ribozyme to cleave alternate substrates, we substituted the w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw401
更新日期：2016-08-19 00:00:00
abstract：:The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx263
更新日期：2017-06-20 00:00:00
abstract：:The function of arginine, cysteine and carboxylic amino acid (glutamic and aspartic) residues of sigma was studied using chemical modification by group specific reagents. Following modification of 3 arginine residues with phenylglyoxal or 3 cysteine residues with N-ethylmaleimide (NEM) sigma activity was lost. Analysi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.9.2701
更新日期：1983-05-11 00:00:00
abstract：:Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx121
更新日期：2017-04-07 00:00:00
abstract：:The SIGnaling Network Open Resource 2.0 (SIGNOR 2.0) is a public repository that stores signaling information as binary causal relationships between biological entities. The captured information is represented graphically as a signed directed graph. Each signaling relationship is associated to an effect (up/down-regul...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz949
更新日期：2020-01-08 00:00:00
abstract：:Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between species by horizontal transfer. Several yeast species contain the VMA1 H...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh734
更新日期：2004-07-27 00:00:00
abstract：:PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.22.10669
更新日期：1988-11-25 00:00:00
abstract：:Well defined biomacromolecular patterns such as binding sites, catalytic sites, specific protein or nucleic acid sequences, etc. precisely modulate many important biological phenomena. We introduce PatternQuery, a web-based application designed for detection and fast extraction of such patterns. The application uses a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv561
更新日期：2015-07-01 00:00:00
abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.16.6607
更新日期：1987-08-25 00:00:00
abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.21.5677
更新日期：1992-11-11 00:00:00
abstract：:The aspartate protease of Mucor pusillus (Mucor pusillus rennin; MPR) is a milk-clotting enzyme used in the cheese industry. The partial amino acid sequence of MPR was determined and oligonucleotide probes were synthesized for cloning of the MPR gene. A clone giving positive hybridization with the probes was selected ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.19.7557
更新日期：1986-10-10 00:00:00
abstract：:The open state of human topoisomerase I has been probed by molecular dynamics simulation, starting from the coordinates of the closed structure of the protein complexed with DNA, after elimination of the 22-bp DNA duplex oligonucleotide. A repulsion force between the two lips of the protein has been introduced for a s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm199
更新日期：2007-01-01 00:00:00
abstract：:Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg272
更新日期：2003-03-15 00:00:00
abstract：:Arabidopsis microRNA (miRNA) genes (MIR) give rise to 20- to 22-nt miRNAs that are generated predominantly by the type III endoribonuclease Dicer-like 1 (DCL1) but do not require any RNA-dependent RNA Polymerases (RDRs) or RNA Polymerase IV (Pol IV). Here, we identify a novel class of non-conserved MIR genes that give...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq590
更新日期：2010-11-01 00:00:00
abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq823
更新日期：2011-02-01 00:00:00
abstract：:Many mutations occur as a result of DNA synthesis past the site of DNA damage by DNA damage bypass polymerases. The frequency and types of mutations not only depend on the nature of the damage, but also on the sequence context, as revealed from analysis of mutation spectra of DNA exposed to mutagens. Herein we report ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn595
更新日期：2008-10-01 00:00:00
abstract：:Recognition of DNA by proteins depends on DNA sequence and structure. Often unanswered is whether the structure of naked DNA persists in a protein-DNA complex, or whether protein binding changes DNA shape. While X-ray structures of protein-DNA complexes are numerous, the structure of naked cognate DNA is seldom availa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky033
更新日期：2018-03-16 00:00:00
abstract：:The hypothesis that DNA strands complementary to the coding strand contain in phase coding sequences has been investigated. Statistical analysis of the 50 genes of bacteriophage T7 shows no significant correlation between patterns of codon usage on the coding and non-coding strands. In Bacillus and yeast genes the cor...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.4.1389
更新日期：1985-02-25 00:00:00
abstract：:The NCBI Assembly database (www.ncbi.nlm.nih.gov/assembly/) provides stable accessioning and data tracking for genome assembly data. The model underlying the database can accommodate a range of assembly structures, including sets of unordered contig or scaffold sequences, bacterial genomes consisting of a single compl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1226
更新日期：2016-01-04 00:00:00
abstract：:Analysis of haplotypes is an important tool in population genetics, familial heredity and gene mapping. Determination of haplotypes of multiple single nucleotide polymorphisms (SNPs) or other simple mutations is time consuming and expensive when analyzing large populations, and often requires the help of computational...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnf062
更新日期：2002-06-15 00:00:00
abstract：:The response to amino acid (AA) limitation of the entire aminoacyl-tRNA synthetase (ARS) gene family revealed that 16/20 of the genes encoding cytoplasmic-localized enzymes are transcriptionally induced by activating transcription factor 4 (Atf4) via C/ebp-Atf-Response-Element (CARE) enhancers. In contrast, only 4/19 ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw667
更新日期：2016-11-16 00:00:00
abstract：:Eukaryotic transcription factor B (TFB) proteins are homologous to KsgA/Dim1 ribosomal RNA (rRNA) methyltransferases. The mammalian TFB1, mitochondrial (TFB1M) factor is an essential protein necessary for mitochondrial gene expression. TFB1M mediates an rRNA modification in the small ribosomal subunit and thus plays a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt547
更新日期：2013-09-01 00:00:00
abstract：:Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5' flaps. It is also involved in the maintenance of telomeres. DNA2 is a gene conserved in eukaryotes, and a putative human ortholog of yeast DNA2 (ScDNA2) ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl070
更新日期：2006-04-04 00:00:00
abstract：:Melting of DNA in a segment bounded at both ends by regions of greater stability during electrophoresis in denaturing gradient gels show complex properties, not accommodated with standard melting theory. Compact bands of some DNA molecules become anomalously broadened at the retardation level in a denaturing gradient,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.14.2775
更新日期：1995-07-25 00:00:00
