Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1.

abstract：:Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected...

journal_title：Nucleic acids research

authors： Sharp PM,Tuohy TM,Mosurski KR

更新日期：1986-07-11 00:00:00

abstract：:Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGC...

journal_title：Nucleic acids research

authors： de Vroom E,Broxterman HJ,Sliedregt LA,van der Marel GA,van Boom JH

更新日期：1988-05-25 00:00:00

abstract：:The MalaCards human disease database (http://www.malacards.org/) is an integrated compendium of annotated diseases mined from 68 data sources. MalaCards has a web card for each of ∼20 000 disease entries, in six global categories. It portrays a broad array of annotation topics in 15 sections, including Summaries, Symp...

journal_title：Nucleic acids research

authors： Rappaport N,Twik M,Plaschkes I,Nudel R,Iny Stein T,Levitt J,Gershoni M,Morrey CP,Safran M,Lancet D

更新日期：2017-01-04 00:00:00

abstract：:The nucleotide sequence of the portion of a Bacillus subtilis (strain PAP115) 3 kb Pst I fragment which contains an endo-beta-1, 4-glucanase gene has been determined. This gene encodes a protein of 499 amino acid residues (Mr = 55,234) with a typical B. subtilis signal peptide. Escherichia coli which has been transfor...

journal_title：Nucleic acids research

authors： MacKay RM,Lo A,Willick G,Zuker M,Baird S,Dove M,Moranelli F,Seligy V

更新日期：1986-11-25 00:00:00

abstract：:Available evidence suggests that Mg2+ ions are involved in reactions catalyzed by hammerhead ribozymes. However, the activity in the presence of exclusively monovalent ions led us to question whether divalent metal ions really function as catalysts when they are present. We investigated ribozyme activity in the presen...

journal_title：Nucleic acids research

authors： Inoue A,Takagi Y,Taira K

更新日期：2004-08-09 00:00:00

abstract：:Histone modifications are fundamental to chromatin structure and transcriptional regulation, and are recognized by a limited number of protein folds. Among these folds are PHD fingers, which are present in most chromatin modification complexes. To date, about 15 PHD finger domains have been structurally characterized,...

journal_title：Nucleic acids research

authors： Slama P,Geman D

更新日期：2011-03-01 00:00:00

abstract：:Over 50 introns have been reported in archaeal rRNA genes (rDNAs), a subset of which nests putative homing endonuclease (HEase) genes. Here, we report the identification and characterization of a novel archaeal LAGLIDADG-type HEase, I-ApeKI [corrected], encoded by the ApeK1.S908 intron within the 16S rDNA of Aeropyrum...

journal_title：Nucleic acids research

authors： Nomura N,Morinaga Y,Shirai N,Sako Y

更新日期：2005-07-26 00:00:00

abstract：:The oligomerization state and mode of binding to DNA of the GIY-YIG endonuclease II (EndoII) from bacteriophage T4 was studied using gel filtration and electrophoretic mobility shift assays with a set of mutants previously found to have altered enzyme activity. At low enzyme/DNA ratios all mutants except one bound to ...

journal_title：Nucleic acids research

authors： Lagerbäck P,Andersson E,Malmberg C,Carlson K

更新日期：2009-10-01 00:00:00

abstract：:We previously constructed a multiribozyme expression vector by combining cis- and trans-acting ribozymes and we showed that several ribozymes, each directed against a different target in the HIV genome and acting independently in a 'shotgun' manner, markedly increased the efficiency of cleavage of HIV RNA in vitro [Oh...

journal_title：Nucleic acids research

authors： Yuyama N,Ohkawa J,Koguma T,Shirai M,Taira K

更新日期：1994-11-25 00:00:00

abstract：:Genetic screens in Saccharomyces cerevisiae have identified the roles of ribosome components, tRNAs and translation factors in translational fidelity. These screens rely on the suppression of altered start codons, nonsense codons or frameshift mutations in genes involved in amino acid or nucleotide metabolism. Many of...

journal_title：Nucleic acids research

authors： Magazinnik T,Anand M,Sattlegger E,Hinnebusch AG,Kinzy TG

更新日期：2005-08-12 00:00:00

abstract：:A screening for human proteins capable of interacting with influenza virus NS1 has been carried out using the two-hybrid genetic trap in yeast. A cDNA corresponding to the human homologue of Drosophila melanogaster Staufen protein (hStaufen) was isolated that fulfilled all genetic controls of the two-hybrid protocol. ...

journal_title：Nucleic acids research

authors： Falcón AM,Fortes P,Marión RM,Beloso A,Ortín J

更新日期：1999-06-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures rapid degradation of mRNAs containing premature translation termination codons (PTCs) in eukaryotes. NMD has been shown to also regulate normal gene expression and thus emerged as one of the key post-transcriptional mechanisms ...

journal_title：Nucleic acids research

authors： Viegas MH,Gehring NH,Breit S,Hentze MW,Kulozik AE

更新日期：2007-01-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes and maintains telomeric DNA. Studies of telomeres and telomerase are facilitated by the large number of linear DNA molecules found in ciliated protozoa, such as Tetrahymena thermophila. To examine the expression of telomerase, we investigated the...

journal_title：Nucleic acids research

authors： Hargrove BW,Bhattacharyya A,Domitrovich AM,Kapler GM,Kirk K,Shippen DE,Kunkel GR

更新日期：1999-11-01 00:00:00

abstract：:Symmetry considerations of the kind of structures which can be generated when dimers of histones f2al-f3 and f2a2-f2b interact lead to the following conclusions: chromatin subunits based on closed-shell structures give rise to discrete, non-interacting nucleoprotein subunits with the histones arranged at random along ...

journal_title：Nucleic acids research

authors： Hyde JE,Walker IO

更新日期：1975-03-01 00:00:00

abstract：:Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...

journal_title：Nucleic acids research

authors： Roca X,Sachidanandam R,Krainer AR

更新日期：2003-11-01 00:00:00

abstract：:We studied fluorescence intensity, polarization and lifetime of some commonly used fluorophores conjugated to oligodeoxyribonucleotides with different primary and secondary structures. We found that fluorescence intensity can increase or decrease upon hybridization of the labeled strand to its complement depending on ...

journal_title：Nucleic acids research

authors： Nazarenko I,Pires R,Lowe B,Obaidy M,Rashtchian A

更新日期：2002-05-01 00:00:00

abstract：:Pyrimidine adducts in cellular DNA arise from modification of the pyrimidine 5,6-double bond by oxidation, reduction or hydration. The biological outcome includes increased mutation rate and potential lethality. A major DNA N:-glycosylase responsible for the excision of modified pyrimidine bases is the base excision r...

journal_title：Nucleic acids research

authors： Yang H,Phan IT,Fitz-Gibbon S,Shivji MK,Wood RD,Clendenin WM,Hyman EC,Miller JH

更新日期：2001-02-01 00:00:00

abstract：:Despite the development of new high-throughput sequencing techniques, microarrays are still attractive tools to study small genome organisms, thanks to sample multiplexing and high-feature densities. However, the oligonucleotide design remains a delicate step for most users. A vast array of software is available to de...

journal_title：Nucleic acids research

authors： Jourdren L,Duclos A,Brion C,Portnoy T,Mathis H,Margeot A,Le Crom S

更新日期：2010-06-01 00:00:00

abstract：:Escherichia coli MutY is an adenine and a weak guanine DNA glycosylase involved in reducing mutagenic effects of 7,8-dihydro-8-oxo-guanine (8-oxoG). The C-terminal domain of MutY is required for 8-oxoG recognition and is critical for mutation avoidance of oxidative damage. To determine which residues of this domain ar...

journal_title：Nucleic acids research

authors： Li L,Lu AL

更新日期：2003-06-15 00:00:00

abstract：:Protein-protein interactions regulate many essential enzymatic processes in the cell. Somatic mutations outside of an enzyme active site can therefore impact cellular function by disruption of critical protein-protein interactions. In our investigation of the cellular impact of the T304I cancer mutation of DNA Polymer...

journal_title：Nucleic acids research

authors： Fang Q,Andrews J,Sharma N,Wilk A,Clark J,Slyskova J,Koczor CA,Lans H,Prakash A,Sobol RW

更新日期：2019-07-09 00:00:00

abstract：:Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication prob...

journal_title：Nucleic acids research

authors： Huang SH,Kobryn K

更新日期：2016-06-20 00:00:00

abstract：:The transcription factor HIV-TF1, which binds to a region about 60 bp upstream from the enhancer of the human immunodeficiency virus-1 (HIV-1), was purified from human B cells. HIV-TF1 had a molecular weight of 39,000. Binding of HIV-TF1 to the HIV long terminal repeat (LTR) activated transcription from the HIV promot...

journal_title：Nucleic acids research

authors： Maekawa T,Sudo T,Kurimoto M,Ishii S

更新日期：1991-09-11 00:00:00

abstract：:The Pathway Tools cellular overview diagram is a visual representation of the biochemical network of an organism. The overview is automatically created from a Pathway/Genome Database describing that organism. The cellular overview includes metabolic, transport and signaling pathways, and other membrane and periplasmic...

journal_title：Nucleic acids research

authors： Paley SM,Karp PD

更新日期：2006-08-07 00:00:00

abstract：:MethBank (http://bigd.big.ac.cn/methbank) is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multi...

journal_title：Nucleic acids research

authors： Li R,Liang F,Li M,Zou D,Sun S,Zhao Y,Zhao W,Bao Y,Xiao J,Zhang Z

更新日期：2018-01-04 00:00:00

abstract：:Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single strand...

journal_title：Nucleic acids research

authors： Cartwright IL,Hutchinson DW

更新日期：1980-04-11 00:00:00

abstract：:Ditercalinium, an antitumor bifunctional intercalator which forms a high affinity reversible complex with DNA, was found to be specifically cytotoxic for polA and lig7 E. coli strains. In the polA strain, the cytotoxic effect of ditercalinium was suppressed by the uvrA mutation. DNA single strand breaks accumulated in...

journal_title：Nucleic acids research

authors： Lambert B,Roques BP,Le Pecq JB

更新日期：1988-02-11 00:00:00

abstract：:DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restric...

journal_title：Nucleic acids research

authors： Simpson VJ,Johnson TE,Hammen RF

更新日期：1986-08-26 00:00:00

abstract：:The human transcription factor EF-1A binds to the purine-rich E1A core enhancer sequence in the adenovirus E1A and E4 and polyomavirus enhancer regions. The consensus binding site for EF-1A resembles that of members of the ets domain protein family. EF-1A activation of transcription requires a dimeric binding site. An...

journal_title：Nucleic acids research

authors： Bolwig GM,Bruder JT,Hearing P

更新日期：1992-12-25 00:00:00

abstract：:In nematodes, the 22 nucleotide (nt) spliced leader (SL) is normally encoded by a multi-copy, tandemly reiterated SL gene and is trans-spliced from SL-RNA onto the 5' end of a subset of mRNAs. We have found that the SL is also encoded at multiple (> 100) orphon genomic sites in the parasitic nematode Angiostrongylus c...

journal_title：Nucleic acids research

authors： Joshua GW,Perler FB,Wang CC

更新日期：1995-03-25 00:00:00

abstract：:The regulation of the Sso II restriction-modification system from Shigella sonnei was studied in vivo and in vitro . In lacZ fusion experiments, Sso II methyltransferase (M. Sso II) was found to repress its own synthesis but stimulate expression of the cognate restriction endonuclease (ENase). The N-terminal 72 amino ...

journal_title：Nucleic acids research

authors： Karyagina A,Shilov I,Tashlitskii V,Khodoun M,Vasil'ev S,Lau PC,Nikolskaya I

更新日期：1997-06-01 00:00:00