Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging.

abstract：:We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II inhibitors in naked versus nucleosome-reconstituted SV40 DNA. DNA fragments were reconstituted with histone octamers from HeLa c...

journal_title：Nucleic acids research

authors： Capranico G,Jaxel C,Roberge M,Kohn KW,Pommier Y

更新日期：1990-08-11 00:00:00

abstract：:Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleoso...

journal_title：Nucleic acids research

authors： Nizovtseva EV,Clauvelin N,Todolli S,Polikanov YS,Kulaeva OI,Wengrzynek S,Olson WK,Studitsky VM

更新日期：2017-04-07 00:00:00

abstract：:A general approach for the synthesis of oligonucleotide-triplet phosphoramidites and the synthesis of four such blocks are described. A strategy was devised to minimize the number of dimer precursors needed for synthesis of a complete set of triplet-amidite blocks encoding all 20 amino acids. Whereas synthesis of 20 t...

journal_title：Nucleic acids research

authors： Ono A,Matsuda A,Zhao J,Santi DV

更新日期：1995-11-25 00:00:00

abstract：:The formation of interstrand cross-links in nucleic acids can have a strong impact on biological function of nucleic acids; therefore, many cross-linking agents have been developed for biological applications. Despite numerous studies, there remains a need for cross-linking agents that exhibit both efficiency and sele...

journal_title：Nucleic acids research

authors： Nishimoto A,Jitsuzaki D,Onizuka K,Taniguchi Y,Nagatsugi F,Sasaki S

更新日期：2013-07-01 00:00:00

abstract：:A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mitochondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs"). The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the co...

journal_title：Nucleic acids research

authors： Hensgens LA,Brakenhoff J,De Vries BF,Sloof P,Tromp MC,Van Boom JH,Benne R

更新日期：1984-10-11 00:00:00

abstract：:The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5...

journal_title：Nucleic acids research

authors： Hashimoto H,Pais JE,Dai N,Corrêa IR Jr,Zhang X,Zheng Y,Cheng X

更新日期：2015-12-15 00:00:00

abstract：:Transcription factor IIIA (TFIIIA) is specifically required for transcription of 5S rRNA genes and is the archetypal C2H2 zinc finger protein. All known vertebrate TFIIIAs have a similar organization: nine zinc fingers, followed by a C-terminal domain of unknown structure. The zinc fingers of Saccharomyces cerevisiae ...

journal_title：Nucleic acids research

authors： Schulman DB,Setzer DR

更新日期：2002-07-01 00:00:00

abstract：:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...

journal_title：Nucleic acids research

authors： Cornelissen M,Vandewiele M

更新日期：1989-01-11 00:00:00

abstract：:Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That ...

journal_title：Nucleic acids research

authors： Kim H,Kang N,Chon KW,Kim S,Lee N,Koo J,Kim MS

更新日期：2015-11-16 00:00:00

abstract：:mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Us...

journal_title：Nucleic acids research

authors： Gupta R,Thomas P,Beddington RS,Rigby PW

更新日期：1998-10-01 00:00:00

abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...

journal_title：Nucleic acids research

authors： Hulla JE,Schneider RP

更新日期：1993-02-11 00:00:00

abstract：:The growing application of gene expression profiling demands powerful yet user-friendly bioinformatics tools to support systems-level data understanding. NetworkAnalyst was first released in 2014 to address the key need for interpreting gene expression data within the context of protein-protein interaction (PPI) netwo...

journal_title：Nucleic acids research

authors： Zhou G,Soufan O,Ewald J,Hancock REW,Basu N,Xia J

更新日期：2019-07-02 00:00:00

abstract：:The alpha/beta-hydrolase fold is characterized by a beta-sheet core of five to eight strands connected by alpha-helices to form a alpha/beta/alpha sandwich. In most of the family members the beta-strands are parallels, but some show an inversion in the order of the first strands, resulting in antiparallel orientation....

journal_title：Nucleic acids research

authors： Hotelier T,Renault L,Cousin X,Negre V,Marchot P,Chatonnet A

更新日期：2004-01-01 00:00:00

abstract：:IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to t...

journal_title：Nucleic acids research

authors： Shi L,Qiu D,Zhao G,Corthesy B,Lees-Miller S,Reeves WH,Kao PN

更新日期：2007-01-01 00:00:00

abstract：:Perturbation and time-course data sets, in combination with computational approaches, can be used to infer transcriptional regulatory networks which ultimately govern the developmental pathways and responses of cells. Here, we individually knocked down the four transcription factors PU.1, IRF8, MYB and SP1 in the huma...

journal_title：Nucleic acids research

authors： Vitezic M,Lassmann T,Forrest AR,Suzuki M,Tomaru Y,Kawai J,Carninci P,Suzuki H,Hayashizaki Y,Daub CO

更新日期：2010-12-01 00:00:00

abstract：:The base excision repair DNA glycosylase MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro-8-oxo-guanine (8-oxoG), thereby preventing G:C to T:A mutations. Biallelic germline mutations in the human MYH gene predispose individuals to multiple colorectal ade...

journal_title：Nucleic acids research

authors： Bai H,Jones S,Guan X,Wilson TM,Sampson JR,Cheadle JP,Lu AL

更新日期：2005-01-26 00:00:00

abstract：:High-throughput sequencing technologies enable direct approaches to catalog and analyze snapshots of the total small RNA content of living cells. Characterization of high-throughput sequencing data requires bioinformatic tools offering a wide perspective of the small RNA transcriptome. Here we present SeqBuster, a hig...

journal_title：Nucleic acids research

authors： Pantano L,Estivill X,Martí E

更新日期：2010-03-01 00:00:00

abstract：:Oligonucleotide-directed mutagenesis was used to introduce an A to C transversion at position 523 in the 16S ribosomal RNA gene of Escherichia coli rrnB operon cloned in plasmid pKK3535. E. coli cells transformed with the mutated plasmid were resistant to streptomycin. The mutated ribosomes isolated from these cells w...

journal_title：Nucleic acids research

authors： Melançon P,Lemieux C,Brakier-Gingras L

更新日期：1988-10-25 00:00:00

abstract：:We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...

journal_title：Nucleic acids research

authors： De BK,Perrault J

更新日期：1982-11-11 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes and maintains telomeric DNA. Studies of telomeres and telomerase are facilitated by the large number of linear DNA molecules found in ciliated protozoa, such as Tetrahymena thermophila. To examine the expression of telomerase, we investigated the...

journal_title：Nucleic acids research

authors： Hargrove BW,Bhattacharyya A,Domitrovich AM,Kapler GM,Kirk K,Shippen DE,Kunkel GR

更新日期：1999-11-01 00:00:00

abstract：:Large-scale automatic annotation of protein sequences remains challenging in postgenomics era. E1DS is designed for annotating enzyme sequences based on a repository of 1D signatures. The employed sequence signatures are derived using a novel pattern mining approach that discovers long motifs consisted of several sequ...

journal_title：Nucleic acids research

authors： Chien TY,Chang DT,Chen CY,Weng YZ,Hsu CM

更新日期：2008-07-01 00:00:00

abstract：:Mammalian gene promoters for transcription by RNA polymerase II are typically organized in the following order: upstream sequence motif(s)/TATA box/initiation site. Here we report studies in which the order, orientation and DNA sequences of these three elements are varied to determine how these affect polarity of tran...

journal_title：Nucleic acids research

authors： Xu LC,Thali M,Schaffner W

更新日期：1991-12-25 00:00:00

abstract：:A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends. ...

journal_title：Nucleic acids research

authors： Steponaviciene D,Maneliene Z,Petrusyte M,Janulaitis A

更新日期：1999-07-01 00:00:00

abstract：:UHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto repli...

journal_title：Nucleic acids research

authors： Li T,Wang L,Du Y,Xie S,Yang X,Lian F,Zhou Z,Qian C

更新日期：2018-04-06 00:00:00

abstract：:Muscleblind-like proteins (MBNLs) are regulators of RNA metabolism. During tissue differentiation the level of MBNLs increases, while their functional insufficiency plays a crucial role in myotonic dystrophy (DM). Deep sequencing of RNA molecules cross-linked to immunoprecipitated protein particles (CLIP-seq) revealed...

journal_title：Nucleic acids research

authors： Konieczny P,Stepniak-Konieczna E,Taylor K,Sznajder LJ,Sobczak K

更新日期：2017-02-28 00:00:00

abstract：:Invertebrate RNA viruses are targets of the host RNA interference (RNAi) pathway, which limits virus infection by degrading viral RNA substrates. Several insect RNA viruses encode suppressor proteins to counteract this antiviral response. We recently demonstrated that the dsDNA virus Invertebrate iridescent virus 6 (I...

journal_title：Nucleic acids research

authors： Bronkhorst AW,van Cleef KW,Venselaar H,van Rij RP

更新日期：2014-10-29 00:00:00

abstract：:The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...

journal_title：Nucleic acids research

authors： Burland V,Plunkett G 3rd,Sofia HJ,Daniels DL,Blattner FR

更新日期：1995-06-25 00:00:00

abstract：:REBASE is a comprehensive database of information about restriction enzymes and their associated methylases, including their recognition and cleavage sites and their commercial availability. Also included is a listing of homing endonucleases. Information from REBASE is available via monthly electronic mailings as well...

journal_title：Nucleic acids research

authors： Roberts RJ,Macelis D

更新日期：1998-01-01 00:00:00

abstract：:Interferon regulatory factors IRF-3 and IRF-7 are transcription factors essential in the activation of interferon-β (IFN-β) gene in response to viral infections. Although, both proteins recognize the same consensus IRF binding site AANNGAAA, they have distinct DNA binding preferences for sites in vivo. The X-ray struc...

journal_title：Nucleic acids research

authors： De Ioannes P,Escalante CR,Aggarwal AK

更新日期：2011-09-01 00:00:00

abstract：:We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...

journal_title：Nucleic acids research

authors： Tsukahara T,Casciato C,Helfman DM

更新日期：1994-06-25 00:00:00