The Pathway Tools cellular overview diagram and Omics Viewer.

abstract：:The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was esta...

journal_title：Nucleic acids research

authors： Earp DJ,Lowe B,Baker B

更新日期：1990-06-11 00:00:00

abstract：:The ability to induce gene expression in a small molecule dependent manner has led to many applications in target discovery, functional elucidation and bio-production. To date these applications have relied on a limited set of protein-based control mechanisms operating at the level of transcription initiation. The dis...

journal_title：Nucleic acids research

authors： Morra R,Shankar J,Robinson CJ,Halliwell S,Butler L,Upton M,Hay S,Micklefield J,Dixon N

更新日期：2016-02-18 00:00:00

abstract：:Intramolecular RNA cross-links were induced within the large ribosomal subunit of E. coli by mild ultraviolet irradiation. Regions of the 23S RNA previously implicated in interactions with ribosomal-bound tRNA were then specifically excised by addressed cleavage using ribonuclease H, in conjunction with synthetic comp...

journal_title：Nucleic acids research

authors： Mitchell P,Osswald M,Schueler D,Brimacombe R

更新日期：1990-08-11 00:00:00

abstract：:The dhp1+ gene of Schizosaccharomyces pombe is a homolog of Saccharomyces cerevisiae HKE1/RAT1/TAP1 gene that is involved in RNA metabolism such as RNA trafficking and RNA synthesis. dhp1+ is also related to S. cerevisiae DST2 (SEP1) that encodes a DNA strand exchange protein required for sporulation and homologous re...

journal_title：Nucleic acids research

authors： Shobuike T,Sugano S,Yamashita T,Ikeda H

更新日期：1995-02-11 00:00:00

abstract：:Double-stranded (ds) oligodeoxynucleotides (29mers) containing an O6-ethylguanine (O6-EtGua) flanked 5' and 3' by different bases (5'..TGT..3'; 5'..CGG..3', 5'..GGT..3'; 5'..GGG..3'; 5'..GGA..3') were synthesized to investigate the binding and repair characteristics of recombinant human O6-alkylguanine-DNA alkyltransf...

journal_title：Nucleic acids research

authors： Bender K,Federwisch M,Loggen U,Nehls P,Rajewsky MF

更新日期：1996-06-01 00:00:00

abstract：:Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2...

journal_title：Nucleic acids research

authors： Singer B,Pergolizzi RG,Grunberger D

更新日期：1979-04-01 00:00:00

abstract：:Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...

journal_title：Nucleic acids research

authors： Oomoto I,Suzuki-Hirano A,Umeshima H,Han YW,Yanagisawa H,Carlton P,Harada Y,Kengaku M,Okamoto A,Shimogori T,Wang DO

更新日期：2015-10-30 00:00:00

abstract：:Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet the mechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATA-binding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcrip...

journal_title：Nucleic acids research

authors： Baumann DG,Gilmour DS

更新日期：2017-10-13 00:00:00

abstract：:Nuclei from frozen calf thymus suspended in buffer were analyzed for metal content prior to and after repeated washing. After three such extractions about 0.1 micrograms Zn/mg DNA and 0.025 micrograms Cu/mg DNA remained tightly associated with chromatin. This level of metal was essentially unchanged with subsequent wa...

journal_title：Nucleic acids research

authors： Bryan SE,Vizard DL,Beary DA,LaBiche RA,Hardy KJ

更新日期：1981-11-11 00:00:00

abstract：:Two factors are mainly responsible for the stability of the DNA double helix: base pairing between complementary strands and stacking between adjacent bases. By studying DNA molecules with solitary nicks and gaps we measure temperature and salt dependence of the stacking free energy of the DNA double helix. For the fi...

journal_title：Nucleic acids research

authors： Yakovchuk P,Protozanova E,Frank-Kamenetskii MD

更新日期：2006-01-31 00:00:00

abstract：:Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...

journal_title：Nucleic acids research

authors： Ilyin Y,Chmeliauskaite VG,Kulguskin VV,Georgiev GP

更新日期：1980-11-25 00:00:00

abstract：:When the function of origins of replication in yeast was compromised by placing ARS sequences downstream of strong promoters, ARS activity might have been affected either by transcription or by an altered chromatin configuration induced by the construct. To distinguish between these possibilities, derivatives of the y...

journal_title：Nucleic acids research

authors： Tanaka S,Halter D,Livingstone-Zatchej M,Reszel B,Thoma F

更新日期：1994-09-25 00:00:00

abstract：:Extracts of the human glioma cell line A1235 (lacking O(6)-methylguanine-DNA methyltransferase) are known to restore a G:T mismatch to a normal G:C pair in a G:T-containing model (45 bp) DNA substrate. Herein we demonstrate that substitution of G:T with O(6)-methylguanine:T (m6G:T) results in extract-induced intra-str...

journal_title：Nucleic acids research

authors： Lari SU,Day RS,Dobler K,Paterson MC

更新日期：2001-06-01 00:00:00

abstract：:Recent studies indicate that controlling the nuclear decondensation and intra-nuclear localization of plasmid DNA (pDNA) would result in an increased transfection efficiency. In the present study, we established a technology for imaging the nuclear condensation/decondensation status of pDNA in nuclear subdomains using...

journal_title：Nucleic acids research

authors： Shaheen SM,Akita H,Yamashita A,Katoono R,Yui N,Biju V,Ishikawa M,Harashima H

更新日期：2011-04-01 00:00:00

abstract：:The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution fol...

journal_title：Nucleic acids research

authors： Jordán-Pla A,Gupta I,de Miguel-Jiménez L,Steinmetz LM,Chávez S,Pelechano V,Pérez-Ortín JE

更新日期：2015-01-01 00:00:00

abstract：:An oligodeoxyribonucleotide, d(GCTCACAAT-X-ATTGTGAGC), where X represents a hexaethylene glycol chain, was studied using circular dichroism spectroscopy. Its conformation and conformational stability were compared to those of compounds where X was replaced by four thymines and to the duplex of same sequence without lo...

journal_title：Nucleic acids research

authors： Durand M,Chevrie K,Chassignol M,Thuong NT,Maurizot JC

更新日期：1990-11-11 00:00:00

abstract：:Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...

journal_title：Nucleic acids research

authors： Jegga AG,Gupta A,Gowrisankar S,Deshmukh MA,Connolly S,Finley K,Aronow BJ

更新日期：2005-07-01 00:00:00

abstract：:Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chro...

journal_title：Nucleic acids research

authors： Kubli E,Schmidt T

更新日期：1978-05-01 00:00:00

abstract：:The MalaCards human disease database (http://www.malacards.org/) is an integrated compendium of annotated diseases mined from 68 data sources. MalaCards has a web card for each of ∼20 000 disease entries, in six global categories. It portrays a broad array of annotation topics in 15 sections, including Summaries, Symp...

journal_title：Nucleic acids research

authors： Rappaport N,Twik M,Plaschkes I,Nudel R,Iny Stein T,Levitt J,Gershoni M,Morrey CP,Safran M,Lancet D

更新日期：2017-01-04 00:00:00

abstract：:In addition to maintaining the GenBank® nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data made available through the NCBI Web site. NCBI resources include Entrez, the Entrez Programming Ut...

journal_title：Nucleic acids research

authors： Sayers EW,Barrett T,Benson DA,Bolton E,Bryant SH,Canese K,Chetvernin V,Church DM,DiCuccio M,Federhen S,Feolo M,Fingerman IM,Geer LY,Helmberg W,Kapustin Y,Landsman D,Lipman DJ,Lu Z,Madden TL,Madej T,Maglott DR,Ma

更新日期：2011-01-01 00:00:00

abstract：:Using total internal reflection fluorescence microscopy, we directly visualize in real-time, the 1D Brownian motion and transcription elongation of T7 RNA polymerase along aligned DNA molecules bound to substrates by molecular combing. We fluorescently label T7 RNA polymerase with antibodies and use flow to convect th...

journal_title：Nucleic acids research

authors： Kim JH,Larson RG

更新日期：2007-01-01 00:00:00

abstract：:A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminop...

journal_title：Nucleic acids research

authors： Damha MJ,Giannaris PA,Zabarylo SV

更新日期：1990-07-11 00:00:00

abstract：:The beta-globin gene of a patient with mRNA-deficient beta o-thalassemia has been sequenced. We find a single nucleotide deletion in amino acid codon 44 that produces a UGA terminator at codon 60. We have previously shown that the beta-globin mRNA of this patient is correctly spliced and polyadenylated, but rapidly tu...

journal_title：Nucleic acids research

authors： Kinniburgh AJ,Maquat LE,Schedl T,Rachmilewitz E,Ross J

更新日期：1982-09-25 00:00:00

abstract：:Structural changes of reconstituted SV 40 minichromosomes have been studied in relation to the salt concentration and addition of histone H1 by sedimentation and electron microscopy. Sedimentation data are represented as functions of the NaCl concentration and the Debye-Hückel electrostatic screening radius 1/alpha. T...

journal_title：Nucleic acids research

authors： Böttger M,Scherneck S,von Mickwitz CU,Fenske H,Lindigkeit R

更新日期：1979-08-10 00:00:00

abstract：:Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...

journal_title：Nucleic acids research

authors： Sparrow DB,Latinkic B,Mohun TJ

更新日期：2000-02-15 00:00:00

abstract：:Natural antisense transcripts are at least partially complementary to their sense transcripts. Cis-Sense/Antisense pairs (cis-SAs) have been extensively characterized and known to play diverse regulatory roles, whereas trans-Sense/Antisense pairs (trans-SAs) in animals are poorly studied. We identified long trans-SAs ...

journal_title：Nucleic acids research

authors： Li JT,Zhang Y,Kong L,Liu QR,Wei L

更新日期：2008-09-01 00:00:00

abstract：:PASMet (Prediction, Analysis and Simulation of Metabolic networks) is a web-based platform for proposing and verifying mathematical models to understand the dynamics of metabolism. The advantages of PASMet include user-friendliness and accessibility, which enable biologists and biochemists to easily perform mathematic...

journal_title：Nucleic acids research

authors： Sriyudthsak K,Mejia RF,Arita M,Hirai MY

更新日期：2016-07-08 00:00:00

abstract：:Myotonic dystrophy type 1 (DM1) is caused by the expansion of a (CTG).(CAG) repeat in the DMPK gene on chromosome 19q13.3. At least 17 neurological diseases have similar genetic mutations, the expansion of DNA repeats. In most of these disorders, the disease severity is related to the length of the repeat expansion, a...

journal_title：Nucleic acids research

authors： Hashem VI,Pytlos MJ,Klysik EA,Tsuji K,Khajavi M,Ashizawa T,Sinden RR

更新日期：2004-12-01 00:00:00

abstract：:In prokaryotes and archaea transfer ribonucleic acid (tRNA) stability as well as cellular UV protection relies on the post-transcriptional modification of uracil at position 8 (U8) of tRNAs by the 4-thiouridine synthetase ThiI. Here, we report three crystal structures of ThiI from Thermotoga maritima in complex with a...

journal_title：Nucleic acids research

authors： Neumann P,Lakomek K,Naumann PT,Erwin WM,Lauhon CT,Ficner R

更新日期：2014-06-01 00:00:00

abstract：:Properties of a mutant bacteriophage T2 DNA [N:(6)-adenine] methyltransferase (T2 Dam MTase) have been investigated for its potential utilization in RecA-assisted restriction endonuclease (RARE) cleavage. Steady-state kinetic analyses with oligonucleotide duplexes revealed that, compared to wild-type T4 Dam, both wild...

journal_title：Nucleic acids research

authors： Minko I,Hattman S,Lloyd RS,Kossykh V

更新日期：2001-04-01 00:00:00