Abstract：
:Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication problem. Deoxyribonucleic acid replication initiates internally and proceeds bidirectionally toward the hairpin telomeres. The telomere resolvase, ResT, forms the hairpin telomeres from replicated telomere intermediates in a reaction with similarities to those promoted by type IB topoisomerases and tyrosine recombinases. ResT has also been shown to possess DNA single-strand annealing activity. We report here that ResT promotes single-strand annealing of both free DNA strands and ssDNA complexed with single-stranded DNA binding protein (SSB). The annealing of complementary strands bound by SSB requires a ResT-SSB interaction that is mediated by the conserved amphipathic C-terminal tail of SSB. These properties of ResT are similar to those demonstrated for the recombination mediator protein, RecO, of the RecF pathway. Borrelia burgdorferi is unusual in lacking identifiable homologs of the RecFOR proteins. We propose that ResT may provide missing RecFOR functions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Huang SH,Kobryn Kdoi
10.1093/nar/gkw344subject
Has Abstractpub_date
2016-06-20 00:00:00pages
5288-98issue
11eissn
0305-1048issn
1362-4962pii
gkw344journal_volume
44pub_type
杂志文章abstract：:We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Gen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.15.3187
更新日期：1994-08-11 00:00:00
abstract：:DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.2.1227
更新日期：1984-01-25 00:00:00
abstract：:The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl994
更新日期：2007-01-01 00:00:00
abstract：:To study the effect of intron size on splicing efficiency we have varied the size of the avian leukosis virus (ALV) env mRNA intron in a cloned ALV genome. This was accomplished by deletion of ALV sequences or insertion of phage lambda DNA. The effect of these modifications on splicing was analyzed by microinjection o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.19.6177
更新日期：1982-10-11 00:00:00
abstract：:The Aspergillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of argB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ans1 or by cotransforming selectable plasmids (c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.22.9163
更新日期：1987-11-25 00:00:00
abstract：:Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.6.823
更新日期：1974-06-01 00:00:00
abstract：:A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.12.4227
更新日期：1985-06-25 00:00:00
abstract：:We describe a database of macromolecular motions meant to be of general use to the structural community. The database, which is accessible on the World Wide Web with an entry point at http://bioinfo.mbb.yale.edu/MolMovDB , attempts to systematize all instances of protein and nucleic acid movement for which there is at...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.18.4280
更新日期：1998-09-15 00:00:00
abstract：:An immunofluorescence assay was developed to identify proteins specifically binding to oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-kappaB p50 protein was incubated with either bead-bound NF-kappaB consensus sequence or a bead-bound ODN combinatorial library and adso...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng054
更新日期：2003-05-15 00:00:00
abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.24.5477
更新日期：1994-12-11 00:00:00
abstract：:Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1-ORF2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl531
更新日期：2006-01-01 00:00:00
abstract：:We present a www server for AUGUSTUS, a novel software program for ab initio gene prediction in eukaryotic genomic sequences. Our method is based on a generalized Hidden Markov Model with a new method for modeling the intron length distribution. This method allows approximation of the true intron length distribution m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh379
更新日期：2004-07-01 00:00:00
abstract：:A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.12.3681
更新日期：1982-06-25 00:00:00
abstract：:Tomato golden mosaic geminivirus has a genome of two single-stranded (ss) DNA components, A and B. An almost identical 'common' region in DNA A and DNA B is thought to contain sequence elements controlling replication and transcription. Hence investigation of sequences important for DNA replication by in vitro mutagen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.24.10213
更新日期：1989-12-25 00:00:00
abstract：:Toxic metals are part of our environment, and undue exposure to them leads to a variety of pathologies. In response, most organisms adapt their metabolism and have evolved systems to limit this toxicity and to acquire tolerance. Ribosome biosynthesis being central for protein synthesis, we analyzed in yeast the effect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt335
更新日期：2013-07-01 00:00:00
abstract：:Dictyostelium intermediate repeat sequence 1 (DIRS-1) is the founding member of a poorly characterized class of retrotransposable elements that contain inverse long terminal repeats and tyrosine recombinase instead of DDE-type integrase enzymes. In Dictyostelium discoideum, DIRS-1 forms clusters that adopt the functio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1337
更新日期：2014-03-01 00:00:00
abstract：:Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.10.1911
更新日期：1975-10-01 00:00:00
abstract：:Increasingly large-scale expression compendia for different species are becoming available. By exploiting the modularity of the coexpression network, these compendia can be used to identify biological processes for which the expression behavior is conserved over different species. However, comparing module networks ac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1275
更新日期：2011-04-01 00:00:00
abstract：:Identification of MHC-binding peptides is a prerequisite in rational design of T-cell based peptide vaccines. During the past decade a number of computational approaches have been introduced for the prediction of MHC-binding peptides, efficiently reducing the number of candidate binders that need to be experimentally ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl284
更新日期：2006-07-01 00:00:00
abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh400
更新日期：2004-07-01 00:00:00
abstract：:Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.3.1609
更新日期：1984-02-10 00:00:00
abstract：:APOBEC3G (A3G), a host protein that inhibits HIV-1 reverse transcription and replication in the absence of Vif, displays cytidine deaminase and single-stranded (ss) nucleic acid binding activities. HIV-1 nucleocapsid protein (NC) also binds nucleic acids and has a unique property, nucleic acid chaperone activity, whic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm750
更新日期：2007-01-01 00:00:00
abstract：:Two dimensional PEI-cellulose thin layer chromatography can resolve sequentially degraded oligonucleotide fragments of tRNA. This technique entails the sequential degradation of the oligonucleotide with snake venom phosphodiesterase in the presence of bacterial alkaline phosphatase, and periodate oxidation followed by...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.10.3563
更新日期：1977-10-01 00:00:00
abstract：:The sequence of the 3'-terminal 180 and 140 nucleotides of RNAs 2 and 3, respectively, of tobacco streak virus (TSV) was deduced by reverse transcription in the presence of a specific primer and chain terminators. Homology between the two RNAs was found to be restricted to a 3-terminal region of about 45 nucleotides. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.15.3307
更新日期：1980-08-11 00:00:00
abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt589
更新日期：2013-09-01 00:00:00
abstract：:The U2 snRNP complex contains two specific proteins, U2B" and U2A'. We have analysed the interaction of U2A' with U2B" and with U2 RNA. U2A' can form an weak but detectable RNA-protein complex with U2 RNA and a stable protein complex with U2B". This protein-protein complex binds efficiently and specifically to U2 RNA....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.3.455
更新日期：1991-02-11 00:00:00
abstract：:Mismatch repair is a highly conserved cellular pathway responsible for repairing mismatched dsDNA. Errors are detected by the MutS enzyme, which most likely senses altered mechanical property of damaged dsDNA rather than a specific molecular pattern. While the curved shape of dsDNA in crystallographic MutS/DNA structu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa896
更新日期：2020-11-18 00:00:00
abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.10.1758
更新日期：1995-05-25 00:00:00
abstract：:Nematode mitochondria expresses two types of extremely truncated tRNAs that are specifically recognized by two distinct elongation factor Tu (EF-Tu) species named EF-Tu1 and EF-Tu2. This is unlike the canonical EF-Tu molecule that participates in the standard protein biosynthesis systems, which basically recognizes al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki784
更新日期：2005-08-19 00:00:00
abstract：:Mammalian gene promoters for transcription by RNA polymerase II are typically organized in the following order: upstream sequence motif(s)/TATA box/initiation site. Here we report studies in which the order, orientation and DNA sequences of these three elements are varied to determine how these affect polarity of tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.24.6699
更新日期：1991-12-25 00:00:00
