Abstract:
:Symmetry considerations of the kind of structures which can be generated when dimers of histones f2al-f3 and f2a2-f2b interact lead to the following conclusions: chromatin subunits based on closed-shell structures give rise to discrete, non-interacting nucleoprotein subunits with the histones arranged at random along the DNA chain; open structures based on infinite helices give rise to highly ordered, regular arrangements of dimers. A model is proposed in which helical polymers of f2al-f3 and f2b-f2a2 form a central core with the DNA helically arranged around it. The helical repeat contains 9.6 turns of B-form DNA and one molecule each of f2al, f2a2, f2b, f3 and f1. The pitch of the helix is 53Angstrom and the other diameter 130Angstrom. The protein molecular repeat is 106Angstrom.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hyde JE,Walker IOdoi
10.1093/nar/2.3.405keywords:
subject
Has Abstractpub_date
1975-03-01 00:00:00pages
405-21issue
3eissn
0305-1048issn
1362-4962journal_volume
2pub_type
杂志文章abstract::Exon arrays are regularly used to analyze differential splicing events. GeneChip Gene 1.0 ST Arrays (gene arrays) manufactured by Affymetrix, Inc. are primarily used to determine expression levels of transcripts, although their basic design is rather similar to GeneChip Exon 1.0 ST Arrays (exon arrays). Here, we show ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1110
更新日期:2012-03-01 00:00:00
abstract::We have sequenced the 0.7-kb-long fragment of Drosophila DNA which ensures the autonomous replication of plasmids in yeast. Deletion mapping has shown the ARS element to consist of at least two domains: the core having the consensus 11-bp sequence TAAATATAAAT and the enhancer which is no more than 90 bp long and is lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.3.1169
更新日期:1988-02-11 00:00:00
abstract::An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.20.4098
更新日期:1996-10-15 00:00:00
abstract::In the virion core of retroviruses, the genomic RNA is tightly associated with nucleocapsid (NC) protein molecules, forming the nucleocapsid structure. NC protein, a highly basic protein with two zinc fingers, is indispensable for RNA dimerization, encapsidation and the initiation of reverse transcription in avian, mu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.4.831
更新日期:1993-02-25 00:00:00
abstract::The RIKEN Arabidopsis Genome Encyclopedia (RARGE) database houses information on biological resources ranging from transcriptome to phenome, including RIKEN Arabidopsis full-length (RAFL) complementary DNAs (cDNAs), their promoter regions, Dissociation (Ds) transposon-tagged lines and expression data from microarray e...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki014
更新日期:2005-01-01 00:00:00
abstract::Histone H2A ubiquitination plays critical roles in transcriptional repression and deoxyribonucleic acid (DNA) damage response. More attention has been focused on ubiquitin E3 ligases of H2A, however, less is known about the negative regulators of H2A ubiquitination. Here we identified HSCARG as a new negative regulato...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku230
更新日期:2014-05-01 00:00:00
abstract::Genome-wide association (GWA) studies to map genes for complex traits are powerful yet costly. DNA-pooling strategies have the potential to dramatically reduce the cost of GWA studies. Pooling using Affymetrix arrays has been proposed and used but the efficiency of these arrays has not been quantified. We compared and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1060
更新日期:2008-04-01 00:00:00
abstract::Guanine rich nucleic acid sequences can form G-quadruplex (G4) structures that interfere with DNA replication, repair and RNA transcription. The human FANCJ helicase contributes to maintaining genomic integrity by promoting DNA replication through G4-forming DNA regions. Here, we combined single-molecule and ensemble ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw574
更新日期:2016-10-14 00:00:00
abstract::Glutaminyl-tRNA synthetase from Deinococcus radiodurans possesses a C-terminal extension of 215 residues appending the anticodon-binding domain. This domain constitutes a paralog of the Yqey protein present in various organisms and part of it is present in the C-terminal end of the GatB subunit of GatCAB, a partner of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1164
更新日期:2007-01-01 00:00:00
abstract::When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of mu heavy chain mRNA increases ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.24.10439
更新日期:1989-12-25 00:00:00
abstract::The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.21.7607
更新日期:1985-11-11 00:00:00
abstract::Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1034
更新日期:2011-03-01 00:00:00
abstract::Genomic imbalances are a major cause of constitutional and acquired disorders. Therefore, aneuploidy screening has become the cornerstone of preimplantation, prenatal and postnatal genetic diagnosis, as well as a routine aspect of the diagnostic workup of many acquired disorders. Recently, array comparative genomic hy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl336
更新日期:2006-05-12 00:00:00
abstract::Many nucleic acid-binding proteins and the AAA+ family form hexameric rings, but the mechanism of hexamer assembly is unclear. It is generally believed that the specificity in protein/RNA interaction relies on molecular contact through a surface charge or 3D structure matching via conformational capture or induced fit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn669
更新日期:2008-11-01 00:00:00
abstract::Pfam is a widely used database of protein families, currently containing more than 13,000 manually curated protein families as of release 26.0. Pfam is available via servers in the UK (http://pfam.sanger.ac.uk/), the USA (http://pfam.janelia.org/) and Sweden (http://pfam.sbc.su.se/). Here, we report on changes that ha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1065
更新日期:2012-01-01 00:00:00
abstract::We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1150
更新日期:2010-03-01 00:00:00
abstract::ProPortal (http://proportal.mit.edu/) is a database containing genomic, metagenomic, transcriptomic and field data for the marine cyanobacterium Prochlorococcus. Our goal is to provide a source of cross-referenced data across multiple scales of biological organization--from the genome to the ecosystem--embracing the f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1022
更新日期:2012-01-01 00:00:00
abstract::The Chinese hamster cell line, DC-3F, is heterozygous at the DHFR locus, and each allele can be distinguished on the basis of a unique DNA restriction pattern, protein isoelectric profile and in the abundancy of the DHFR mRNAs it expresses. Although each allele produces four transcripts, 1000, 1650 and 2150 nucleotide...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.24.6597
更新日期:1992-12-25 00:00:00
abstract::The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD act...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.8.3619
更新日期:1984-04-25 00:00:00
abstract::Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.11.2077
更新日期:1975-11-01 00:00:00
abstract::In previous studies, we reported that fractionation of HeLa cell nuclear extracts on glycerol gradients revealed an endogenous ∼10S particle that contained galectin-3 and U1 snRNP and this particle was sufficient to load the galectin polypeptide onto a pre-mRNA substrate. We now document that this interaction between ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw303
更新日期:2016-07-27 00:00:00
abstract::Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.13.5265
更新日期:1984-07-11 00:00:00
abstract::The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variabl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.12.2777
更新日期:1981-06-25 00:00:00
abstract::The microbial genome database for comparative analysis (MBGD) (available at http://mbgd.genome.ad.jp/) is a comprehensive ortholog database for flexible comparative analysis of microbial genomes, where the users are allowed to create an ortholog table among any specified set of organisms. Because of the rapid increase...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1152
更新日期:2015-01-01 00:00:00
abstract::The RNA sequences boxA, boxB and boxC constitute the nut regions of phage lambda. They nucleate the formation of a termination-resistant RNA polymerase complex on the lambda chromosome. The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein. A complex that includes the Nus proteins a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp452
更新日期:2009-08-01 00:00:00
abstract::The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.222
更新日期:1997-01-01 00:00:00
abstract::Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg830
更新日期:2003-11-01 00:00:00
abstract::IslandViewer (http://pathogenomics.sfu.ca/islandviewer) is a web-accessible application for the computational prediction and analysis of genomic islands (GIs) in bacterial and archaeal genomes. GIs are clusters of genes of probable horizontal origin and are of high interest because they disproportionately encode virul...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt394
更新日期:2013-07-01 00:00:00
abstract::A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.19.5765
更新日期:1982-10-11 00:00:00
abstract::In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.16.3141
更新日期:1995-08-25 00:00:00