HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation.

abstract：:DNA methylation and chromatin DNaseI sensitivity were analyzed in and adjacent to D4Z4 repeat arrays, which consist of 1 to approximately 100 tandem 3.3-kb units at subtelomeric 4q and 10q. D4Z4 displayed hypomethylation in some cancers and hypermethylation in others relative to normal tissues. Surprisingly, in cancer...

journal_title：Nucleic acids research

authors： Tsumagari K,Qi L,Jackson K,Shao C,Lacey M,Sowden J,Tawil R,Vedanarayanan V,Ehrlich M

更新日期：2008-04-01 00:00:00

abstract：:The human 7SL RNA component of the signal recognition particle can be separated into four major conformers by nondenaturing polyacrylamide gel electrophoresis. We have investigated what sequences give 7SL RNA the property to exist in different conformations. The human 7SL gene 7L30.1 was mutagenized using the random l...

journal_title：Nucleic acids research

authors： Zwieb C,Ullu E

更新日期：1986-06-11 00:00:00

abstract：:LNA oligonucleotides constitute a class of bicyclic RNA analogues having an exceptionally high affinity for their complementary DNA and RNA target molecules. We here report a novel method for highly efficient isolation of intact poly(A)+ RNA using an LNA-substituted oligo(dT) affinity ligand, based on the increased af...

journal_title：Nucleic acids research

authors： Jacobsen N,Nielsen PS,Jeffares DC,Eriksen J,Ohlsson H,Arctander P,Kauppinen S

更新日期：2004-04-19 00:00:00

abstract：:Bleomycin B(2)(BLM) in the presence of iron [Fe(II)] and O(2)catalyzes single-stranded (ss) and double-stranded (ds) cleavage of DNA. Electrospray ionization ion trap mass spectrometry was used to monitor these cleavage processes. Two duplex oligonucleotides containing an ethylene oxide tether between both strands wer...

journal_title：Nucleic acids research

authors： Harsch A,Marzilli LA,Bunt RC,Stubbe J,Vouros P

更新日期：2000-05-01 00:00:00

abstract：:It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their...

journal_title：Nucleic acids research

authors： Elli R,Marcucci L,Bosi R,Oppi C,Battaglia PA,Gigliani F

更新日期：1983-06-11 00:00:00

abstract：:The eukaryotic mismatch repair protein Msh6 shares five domains in common with other MutS members. However, it also contains several hundred additional residues at its N-terminus. A few of these residues bind to PCNA, but the functions of the other amino acids in the N-terminal region (NTR) are unknown. Here we demons...

journal_title：Nucleic acids research

authors： Clark AB,Deterding L,Tomer KB,Kunkel TA

更新日期：2007-01-01 00:00:00

abstract：:Symmetry considerations of the kind of structures which can be generated when dimers of histones f2al-f3 and f2a2-f2b interact lead to the following conclusions: chromatin subunits based on closed-shell structures give rise to discrete, non-interacting nucleoprotein subunits with the histones arranged at random along ...

journal_title：Nucleic acids research

authors： Hyde JE,Walker IO

更新日期：1975-03-01 00:00:00

abstract：:Thiol-activated neocarzinostatin chromophore abstracts tritium from the 5', but not from the 1' or 2' positions of deoxyribose in DNA and incorporates it into a stable, non-exchangeable form. The abstracted tritium remains covalently associated with the chromophore or its degradation product after treatment with acid ...

journal_title：Nucleic acids research

authors： Kappen LS,Goldberg IH

更新日期：1985-03-11 00:00:00

abstract：:The histone variant H2A.Z has been implicated in many biological processes, such as gene regulation and genome stability. Here, we present the identification of H2A.Z.2.2 (Z.2.2), a novel alternatively spliced variant of histone H2A.Z and provide a comprehensive characterization of its expression and chromatin incorpo...

journal_title：Nucleic acids research

authors： Bönisch C,Schneider K,Pünzeler S,Wiedemann SM,Bielmeier C,Bocola M,Eberl HC,Kuegel W,Neumann J,Kremmer E,Leonhardt H,Mann M,Michaelis J,Schermelleh L,Hake SB

更新日期：2012-07-01 00:00:00

abstract：:RNA-Seq is gradually becoming the standard tool for transcriptomic expression studies in biological research. Although considerable progress has been recorded in the development of statistical algorithms for the detection of differentially expressed genes using RNA-Seq data, the list of detected genes can differ signi...

journal_title：Nucleic acids research

authors： Moulos P,Hatzis P

更新日期：2015-02-27 00:00:00

abstract：:MPromDb (Mammalian Promoter Database) is a curated database that strives to annotate gene promoters identified from ChIP-seq results with the goal of providing an integrated resource for mammalian transcriptional regulation and epigenetics. We analyzed 507 million uniquely aligned RNAP-II ChIP-seq reads from 26 differ...

journal_title：Nucleic acids research

authors： Gupta R,Bhattacharyya A,Agosto-Perez FJ,Wickramasinghe P,Davuluri RV

更新日期：2011-01-01 00:00:00

abstract：:Characterization of the epigenetic status of individual cells remains a challenge. Current sequencing approaches have limited coverage, and it is difficult to assign an epigenetic status to the transcription state of individual gene alleles in the same cell. To address these limitations, a targeted microscopy-based ep...

journal_title：Nucleic acids research

authors： Kint S,Van Criekinge W,Vandekerckhove L,De Vos WH,Bomsztyk K,Krause DS,Denisenko O

更新日期：2021-01-28 00:00:00

abstract：:Chelation of divalent cations by 5 mM EDTA and subsequent removal by dialysis from a cytoplasmic HeLa cell extract leads to a complete loss of 5S rRNA transcription without affecting tRNA synthesis. Transcription complexes for 5S RNA can no longer be assembled in such a zinc-depleted extract and this ability can be fu...

journal_title：Nucleic acids research

authors： Wingender E,Dilloo D,Seifart KH

更新日期：1984-12-11 00:00:00

abstract：:Escherichia coli Exonuclease I (ExoI) digests single-stranded DNA (ssDNA) in the 3'-5' direction in a highly processive manner. The crystal structure of ExoI, determined previously in the absence of DNA, revealed a C-shaped molecule with three domains that form a central positively charged groove. The active site is a...

journal_title：Nucleic acids research

authors： Korada SK,Johns TD,Smith CE,Jones ND,McCabe KA,Bell CE

更新日期：2013-06-01 00:00:00

abstract：:The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. This brings the total of contiguous sequence from the E.coli genome project to 725.1 kb (76.0 to 92.8 minutes). We found 191 putative coding genes (ORFs) of which 72 genes w...

journal_title：Nucleic acids research

authors： Sofia HJ,Burland V,Daniels DL,Plunkett G 3rd,Blattner FR

更新日期：1994-07-11 00:00:00

abstract：:We describe the further development of a widely used package of DNA/protein sequence analysis programs (1). Important revisions have been made based on user experience, and new features, multi-user capability, and a set of large scale homology programs have been added. The programs are very user friendly, economical o...

journal_title：Nucleic acids research

authors： Pustell J,Kafatos FC

更新日期：1984-01-11 00:00:00

abstract：:8-Oxo-7,8-dihydroguanine (8-oxoGua) is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia col...

journal_title：Nucleic acids research

authors： Ishibashi T,Hayakawa H,Ito R,Miyazawa M,Yamagata Y,Sekiguchi M

更新日期：2005-07-07 00:00:00

abstract：:GAP-43 is a membrane phosphoprotein that is important for the development and plasticity of neural connections. In undifferentiated PC12 pheochromocytoma cells, GAP-43 mRNA degrades rapidly ( t = 5 h), but becomes stable when cells are treated with nerve growth factor. To identify trans- acting factors that may influe...

journal_title：Nucleic acids research

authors： Irwin N,Baekelandt V,Goritchenko L,Benowitz LI

更新日期：1997-03-15 00:00:00

abstract：:The pyrrole-imidazole (Py-Im) triamide-cyclopropa pyrroloindole (CPI) conjugates ImPyImLDu86 (7) and ImImPyLDu86 (14) were synthesized and their alkylating activities and inhibitory effects on DNA hydrolysis by restriction endonucleases were examined. Sequencing gel analysis demonstrated that conjugates 7 and 14 speci...

journal_title：Nucleic acids research

authors： Fujimoto K,Iida H,Kawakami M,Bando T,Tao ZF,Sugiyama H

更新日期：2002-09-01 00:00:00

abstract：:Highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5S rRNAs are complementary to the 5' strand of the m2(6)A hairpin structure near the 3' ends of 18S rRNA and 16S rRNA, respectively. The extent of base-pairing and the calculated stabilities of the hybrids that c...

journal_title：Nucleic acids research

authors： Azad AA

更新日期：1979-12-11 00:00:00

abstract：:High-throughput sequencing technologies enable direct approaches to catalog and analyze snapshots of the total small RNA content of living cells. Characterization of high-throughput sequencing data requires bioinformatic tools offering a wide perspective of the small RNA transcriptome. Here we present SeqBuster, a hig...

journal_title：Nucleic acids research

authors： Pantano L,Estivill X,Martí E

更新日期：2010-03-01 00:00:00

abstract：:Bacterial Rho-independent terminators (RITs) are important genomic landmarks involved in gene regulation and terminating gene expression. In this investigation we present RNIE, a probabilistic approach for predicting RITs. The method is based upon covariance models which have been known for many years to be the most a...

journal_title：Nucleic acids research

authors： Gardner PP,Barquist L,Bateman A,Nawrocki EP,Weinberg Z

更新日期：2011-08-01 00:00:00

abstract：:Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....

journal_title：Nucleic acids research

authors： Hinton DM,Musso RE

更新日期：1982-08-25 00:00:00

abstract：:Sequence heterogeneity is a common characteristic of RNA viruses that is often referred to as sub-populations or quasispecies. Traditional techniques used for assembly of short sequence reads produced by deep sequencing, such as de-novo assemblers, ignore the underlying diversity. Here, we introduce a novel algorithm ...

journal_title：Nucleic acids research

authors： Karagiannis K,Simonyan V,Chumakov K,Mazumder R

更新日期：2017-11-02 00:00:00

abstract：:Use of antisense oligonucleotides is a versatile strategy for achieving control of gene expression. Unfortunately, the interpretation of antisense-induced phenotypes is sometimes difficult, and chemical modifications that improve the potency and specificity of antisense action would be useful. The introduction of lock...

journal_title：Nucleic acids research

authors： Braasch DA,Liu Y,Corey DR

更新日期：2002-12-01 00:00:00

abstract：:The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity elect...

journal_title：Nucleic acids research

authors： Dubreuil YL,Expert-Bezançon A,Favre A

更新日期：1991-07-11 00:00:00

abstract：:Histone proteins are synthesized in large amounts during S-phase to package the newly replicated DNA, and are among the most stable proteins in the cell. The replication-dependent (RD)-histone mRNAs expressed during S-phase end in a conserved stem-loop rather than a polyA tail. In addition, there are replication-indep...

journal_title：Nucleic acids research

authors： Lyons SM,Cunningham CH,Welch JD,Groh B,Guo AY,Wei B,Whitfield ML,Xiong Y,Marzluff WF

更新日期：2016-11-02 00:00:00

abstract：:We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...

journal_title：Nucleic acids research

authors： De BK,Perrault J

更新日期：1982-11-11 00:00:00

abstract：:We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reprod...

journal_title：Nucleic acids research

authors： Saris CJ,Franssen HJ,Heuyerjans JH,van Eenbergen J,Bloemers HP

更新日期：1982-08-25 00:00:00

abstract：:We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by the restriction enzym...

journal_title：Nucleic acids research

authors： Germann MW,Kalisch BW,Lundberg P,Vogel HJ,van de Sande JH

更新日期：1990-03-25 00:00:00